Jibin Sun
YOU?
Author Swipe
View article: Putative membrane protein Cgl2168 and its variant enhance thermotolerance in Corynebacterium glutamicum
Putative membrane protein Cgl2168 and its variant enhance thermotolerance in Corynebacterium glutamicum Open
Corynebacterium glutamicum ATCC 13032 is widely used as the chassis strain for industrial production of various amino acids, but its thermotolerance limits productivity under high temperatures. Comparative genomic analysis in this s…
View article: Analysis and Prediction of Building Deformation Characteristics Induced by Geological Hazards
Analysis and Prediction of Building Deformation Characteristics Induced by Geological Hazards Open
To address the building settlement issues induced by an urban geological hazard in a northern city, this study utilizes settlement monitoring data from 16 high-rise buildings. The non-uniform temporal data were processed using the Akima in…
View article: Elucidating the Mechanism of Temporal Adaptation to Hydrogen Peroxide‐Induced Oxidative Stress in <i>Corynebacterium glutamicum</i>
Elucidating the Mechanism of Temporal Adaptation to Hydrogen Peroxide‐Induced Oxidative Stress in <i>Corynebacterium glutamicum</i> Open
Corynebacterium glutamicum serves as a pivotal industrial chassis for biomanufacturing and an ideal model for studying the phylogenetically related pathogen Mycobacterium tuberculosis . Oxidative stress poses a critical challenge to microo…
View article: Engineering of l-threonine and l-proline biosensors by directed evolution of transcriptional regulator SerR and application for high-throughput screening
Engineering of l-threonine and l-proline biosensors by directed evolution of transcriptional regulator SerR and application for high-throughput screening Open
Amino acids are important bio-based products with a multi-billion-dollar market. The development of efficient high-throughput screening technologies utilizing biosensors is essential for the rapid identification of high-performance amino a…
View article: Promoter engineering with programmable upstream activating sequences in Aspergillus Niger cell factory
Promoter engineering with programmable upstream activating sequences in Aspergillus Niger cell factory Open
View article: Using a Novel Small-scale Model to Investigate Mechanical Behaviours of Full-scale Shield Tunnels
Using a Novel Small-scale Model to Investigate Mechanical Behaviours of Full-scale Shield Tunnels Open
A novel small-scale model was proposed to investigate the mechanical behaviour of the full-scale shield tunnels. Because it used a fine-aggregate grout material, the dimensions of the model can be significantly scaled down, therefore, show…
View article: Reconstruction the feedback regulation of amino acid metabolism to develop a non-auxotrophic l-threonine producing Corynebacterium glutamicum
Reconstruction the feedback regulation of amino acid metabolism to develop a non-auxotrophic l-threonine producing Corynebacterium glutamicum Open
l -Threonine is an important feed additive with the third largest market size among the amino acids produced by microbial fermentation. The GRAS (generally regarded as safe) industrial workhorse Corynebacterium glutamicum is an attractive …
View article: Engineering of cofactor preference and catalytic activity of methanol dehydrogenase by growth-coupled directed evolution
Engineering of cofactor preference and catalytic activity of methanol dehydrogenase by growth-coupled directed evolution Open
Methanol, produced from carbon dioxide, natural gas, and biomass, has drawn increasing attention as a promising green carbon feedstock for biomanufacturing due to its sustainable and energy-rich properties. Nicotinamide adenine dinucleotid…
View article: Automated and integrated ultrahigh throughput screening for industrial strain enabled by acoustic-droplet-ejection mass spectrometry
Automated and integrated ultrahigh throughput screening for industrial strain enabled by acoustic-droplet-ejection mass spectrometry Open
Image 1.
View article: Engineering allosteric inhibition of homoserine dehydrogenase by semi-rational saturation mutagenesis screening
Engineering allosteric inhibition of homoserine dehydrogenase by semi-rational saturation mutagenesis screening Open
Allosteric regulation by pathway products plays a vital role in amino acid metabolism. Homoserine dehydrogenase (HSD), the key enzyme for the biosynthesis of various aspartate family amino acids, is subject to feedback inhibition by l -thr…
View article: Elucidating the Mechanism of Temporal Adaptation to Hydrogen Peroxide-Induced Oxidative Stress in Corynebacterium Glutamicum
Elucidating the Mechanism of Temporal Adaptation to Hydrogen Peroxide-Induced Oxidative Stress in Corynebacterium Glutamicum Open
View article: Genome-wide transcription landscape of citric acid producing Aspergillus niger in response to glucose gradient
Genome-wide transcription landscape of citric acid producing Aspergillus niger in response to glucose gradient Open
Aspergillus niger is the main industrial workhorse for global citric acid production. This fungus has complex sensing and signaling pathways to respond to environmental nutrient fluctuations. As the preferred primary carbon source, glucose…
View article: Development of a growth-coupled selection platform for directed evolution of heme biosynthetic enzymes in Corynebacterium glutamicum
Development of a growth-coupled selection platform for directed evolution of heme biosynthetic enzymes in Corynebacterium glutamicum Open
Heme is an important tetrapyrrole compound, and has been widely applied in food and medicine industries. Although microbial production of heme has been developed with metabolic engineering strategies during the past 20 years, the productio…
View article: Engineering of the DNA replication and repair machinery to develop binary mutators for rapid genome evolution of <i>Corynebacterium glutamicum</i>
Engineering of the DNA replication and repair machinery to develop binary mutators for rapid genome evolution of <i>Corynebacterium glutamicum</i> Open
Corynebacterium glutamicum is an important industrial workhorse for production of amino acids and chemicals. Although recently developed genome editing technologies have advanced the rational genetic engineering of C. glutamicum, continuou…
View article: Correction: Systems metabolic engineering of Escherichia coli for hyper-production of 5-aminolevulinic acid
Correction: Systems metabolic engineering of Escherichia coli for hyper-production of 5-aminolevulinic acid Open
View article: Directed evolution of a neutrophilic and mesophilic methanol dehydrogenase based on high-throughput and accurate measurement of formaldehyde
Directed evolution of a neutrophilic and mesophilic methanol dehydrogenase based on high-throughput and accurate measurement of formaldehyde Open
Methanol is a promising one-carbon feedstock for biomanufacturing, which can be sustainably produced from carbon dioxide and natural gas. However, the efficiency of methanol bioconversion is limited by the poor catalytic properties of nico…
View article: Regression modelling of conditional morphogene expression links and quantifies the impact of growth rate, fitness and macromorphology with protein secretion in Aspergillus niger
Regression modelling of conditional morphogene expression links and quantifies the impact of growth rate, fitness and macromorphology with protein secretion in Aspergillus niger Open
View article: Sustainable and high-level microbial production of plant hemoglobin in Corynebacterium glutamicum
Sustainable and high-level microbial production of plant hemoglobin in Corynebacterium glutamicum Open
Background Plant hemoglobin shows great potential as a food additive to circumvent the controversy of using animal materials. Microbial fermentation with engineered microorganisms is considered as a promising strategy for sustainable produ…
View article: Genome-scale CRISPRi screening: A powerful tool in engineering microbiology
Genome-scale CRISPRi screening: A powerful tool in engineering microbiology Open
Deciphering gene function is fundamental to engineering of microbiology. The clustered regularly interspaced short palindromic repeats (CRISPR) system has been adapted for gene repression across a range of hosts, creating a versatile tool …
View article: Systems metabolic engineering of Escherichia coli for hyper-production of 5‑aminolevulinic acid
Systems metabolic engineering of Escherichia coli for hyper-production of 5‑aminolevulinic acid Open
View article: Additional file 2 of Sustainable and high-level microbial production of plant hemoglobin in Corynebacterium glutamicum
Additional file 2 of Sustainable and high-level microbial production of plant hemoglobin in Corynebacterium glutamicum Open
Additional file 2: Table S4. Differentially expressed genes caused by overproduction of hemoglobin Lba-Ec.
View article: Quantitative phenotypic screens of Aspergillus niger mutants in solid and liquid culture
Quantitative phenotypic screens of Aspergillus niger mutants in solid and liquid culture Open
This protocol describes procedures for quantifying Aspergillus niger growth in both solid and liquid culture. Firstly, by comparing radial growth between mutant and progenitor isolates on solid agar supplemented with sublethal stressors, s…
View article: Protocol for gene characterization in Aspergillus niger using 5S rRNA-CRISPR-Cas9-mediated Tet-on inducible promoter exchange
Protocol for gene characterization in Aspergillus niger using 5S rRNA-CRISPR-Cas9-mediated Tet-on inducible promoter exchange Open
View article: Evaluation of Aspergillus niger Six Constitutive Strong Promoters by Fluorescent-Auxotrophic Selection Coupled with Flow Cytometry: A Case for Citric Acid Production
Evaluation of Aspergillus niger Six Constitutive Strong Promoters by Fluorescent-Auxotrophic Selection Coupled with Flow Cytometry: A Case for Citric Acid Production Open
Aspergillus niger is an important industrial workhorse for the biomanufacturing of organic acids, proteins, etc. Well-controlled genetic regulatory elements, including promoters, are vital for strain engineering, but available strong promo…
View article: Comprehensively dissecting the hub regulation of PkaC on high‐productivity and pellet macromorphology in citric acid producing <i>Aspergillus niger</i>
Comprehensively dissecting the hub regulation of PkaC on high‐productivity and pellet macromorphology in citric acid producing <i>Aspergillus niger</i> Open
Summary Aspergillus niger , an important industrial workhorse for citric acid production, is characterized by polar hyphal growth with complex pelleted, clumped or dispersed macromorphologies in submerged culture. Although organic acid tit…
View article: CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an l-proline exporter for l-proline hyperproduction
CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an l-proline exporter for l-proline hyperproduction Open
View article: A Library of Aspergillus niger Chassis Strains for Morphology Engineering Connects Strain Fitness and Filamentous Growth With Submerged Macromorphology
A Library of Aspergillus niger Chassis Strains for Morphology Engineering Connects Strain Fitness and Filamentous Growth With Submerged Macromorphology Open
Submerged fermentation using filamentous fungal cell factories is used to produce a diverse portfolio of useful molecules, including food, medicines, enzymes, and platform chemicals. Depending on strain background and abiotic culture condi…
View article: Data-Driven Synthetic Cell Factories Development for Industrial Biomanufacturing
Data-Driven Synthetic Cell Factories Development for Industrial Biomanufacturing Open
Revolutionary breakthroughs in artificial intelligence (AI) and machine learning (ML) have had a profound impact on a wide range of scientific disciplines, including the development of artificial cell factories for biomanufacturing. In thi…
View article: Impact of Contact Loss On Deformation and Failure of Shield Tunnels
Impact of Contact Loss On Deformation and Failure of Shield Tunnels Open
View article: Development of a Hyperosmotic Stress Inducible Gene Expression System by Engineering the MtrA/MtrB-Dependent NCgl1418 Promoter in Corynebacterium glutamicum
Development of a Hyperosmotic Stress Inducible Gene Expression System by Engineering the MtrA/MtrB-Dependent NCgl1418 Promoter in Corynebacterium glutamicum Open
Corynebacterium glutamicum is an important workhorse for industrial production of diversiform bioproducts. Precise regulation of gene expression is crucial for metabolic balance and enhancing production of target molecules. Auto-inducible …