Suzanne E. Wollen-Roberts

A Single-shot ChAd3 Vaccine Provides Protection from Intramuscular and Aerosol Sudan Virus Exposure Open

Anna N. Honko, Ruth Hunegnaw, Juan I. Moliva, Aurélie Ploquin, Caitlyn N M Dulan , et al. · 2024

Infection with Sudan virus (SUDV) is characterized by an aggressive disease course with case fatality rates between 40-100% and no approved vaccines or therapeutics. SUDV causes sporadic outbreaks in sub-Saharan Africa, including a recent …

Priming with Japanese encephalitis virus or yellow fever virus vaccination led to the recognition of multiple flaviviruses without boosting antibody responses induced by an inactivated Zika virus vaccine Open

Yifan Li, Mélanie Merbah, Suzanne E. Wollen-Roberts, Bradley Beckman, Thembi Mdluli , et al. · 2023

Clade-Specific MPXV PCR Assays Open

Daniel Negrón, Nicholas Tolli, Stephanie Guertin, Suzanne E. Wollen-Roberts, Shane Mitchell , et al. · 2023

In an evolving infectious disease outbreak, there are two priorities: rapid and accurate detection of the causative agent and characterization of its spread. The polymerase chain reaction (PCR) assay is an effective and portable diagnostic…

Coronavirus Antibody Responses before COVID-19 Pandemic, Africa and Thailand Open

Yifan Li, Mélanie Merbah, Suzanne E. Wollen-Roberts, Bradley Beckman, Thembi Mdluli , et al. · 2022

Prior immune responses to coronaviruses might affect human SARS-CoV-2 response. We screened 2,565 serum and plasma samples collected from 2013 through early 2020, before the COVID-19 pandemic began, from 2,250 persons in 4 countries in Afr…

Evaluation of Antibody-Dependent Fc-Mediated Viral Entry, as Compared With Neutralization, in SARS-CoV-2 Infection Open

Lindsay Wieczorek, Michelle Zemil, Mélanie Merbah, Vincent Dussupt, Erin Kavusak , et al. · 2022

Fc-mediated virus entry has been observed for many viruses, but the characterization of this activity in convalescent plasma against SARS-CoV-2 Variants of Concern (VOC) is undefined. In this study, we evaluated Fc-mediated viral entry (FV…

Delayed Disease in Cynomolgus Macaques Exposed to Ebola Virus by an Intranasal Route Open

Sara C. Johnston, Catherine L. Wilhelmsen, Joshua D. Shamblin, Adrienne E. Kimmel, Justine M. Zelko , et al. · 2021

Ebola virus remains a significant public health concern due to high morbidity and mortality rates during recurrent outbreaks in endemic areas. Therefore, the development of countermeasures against Ebola virus remains a high priority, and r…

The utilization of advance telemetry to investigate critical physiological parameters including electroencephalography in cynomolgus macaques following aerosol challenge with eastern equine encephalitis virus Open

John C. Trefry, Franco Rossi, Michael V. Accardi, Brandi Dorsey, Thomas R. Sprague , et al. · 2021

Most alphaviruses are mosquito-borne and can cause severe disease in humans and domesticated animals. In North America, eastern equine encephalitis virus (EEEV) is an important human pathogen with case fatality rates of 30–90%. Currently, …

Transcriptomic Analysis Reveals Host miRNAs Correlated with Immune Gene Dysregulation during Fatal Disease Progression in the Ebola Virus Cynomolgus Macaque Disease Model Open

Christopher P. Stefan, Catherine Arnold, Charles J. Shoemaker, Elizabeth E. Zumbrun, Louis A. Altamura , et al. · 2021

Ebola virus is a continuing threat to human populations, causing a virulent hemorrhagic fever disease characterized by dysregulation of both the innate and adaptive host immune responses. Severe cases are distinguished by an early, elevate…

The Utilization of Advance Telemetry to Investigate Important Physiological Parameters Including Electroencephalography in Cynomolgus Macaques Following Aerosol Challenge with Eastern Equine Encephalitis Virus Open

John C. Trefry, Franco Rossi, Michael V. Accardi, Brandi Dorsey, Thomas R. Sprague , et al. · 2020

Most alphaviruses are mosquito-borne and can cause severe disease in humans and domesticated animals. In North America, eastern equine encephalitis virus (EEEV) is an important human pathogen with case fatality rates of 30-90%. Currently, …

A high-throughput multiplex assay to characterize flavivirus-specific immunoglobulins Open

Mélanie Merbah, Suzanne E. Wollen-Roberts, Zhanna Shubin, Yifan Li, Hongjun Bai , et al. · 2020

Genus Flavivirus, which includes 53 virus species, is the leading cause of arthropod-borne diseases in humans. Diagnosis of these viral diseases is complicated by their overlapping epidemiology and clinical manifestations, and the fact tha…

Modeling mosquito-borne and sexual transmission of Zika virus in an enzootic host, the African green monkey Open

Andrew D. Haddow, Unai Pérez‐Sautu, Michael R. Wiley, Lynn J. Miller, Adrienne E. Kimmel , et al. · 2020

Mosquito-borne and sexual transmission of Zika virus (ZIKV), a TORCH pathogen, recently initiated a series of large epidemics throughout the Tropics. Animal models are necessary to determine transmission risk and study pathogenesis, as wel…

Intranasal monkeypox marmoset model: Prophylactic antibody treatment provides benefit against severe monkeypox virus disease Open

Eric M. Mucker, Suzanne E. Wollen-Roberts, Adrienne E. Kimmel, Josh Shamblin, Darryl Sampey , et al. · 2018

Concerns regarding outbreaks of human monkeypox or the potential reintroduction of smallpox into an immunological naïve population have prompted the development of animal models and countermeasures. Here we present a marmoset model of monk…

Development of Clinical-Stage Human Monoclonal Antibodies That Treat Advanced Ebola Virus Disease in Nonhuman Primates Open

Kristen E. Pascal, Drew Dudgeon, John C. Trefry, Manu Anantpadma, Yasuteru Sakurai , et al. · 2018

This antibody cocktail provides complementary mechanisms of actions, incorporates novel specificities, and demonstrates high-level postexposure protection from lethal EBOV disease in NHPs. It is now undergoing testing in normal healthy vol…

Virus-encoded miRNAs in Ebola virus disease Open

Janice Duy, Anna N. Honko, Louis A. Altamura, Sandra L. Bixler, Suzanne E. Wollen-Roberts , et al. · 2018

A conserved transcriptional response to intranasal Ebola virus exposure in nonhuman primates prior to onset of fever Open

Emily Speranza, Sandra L. Bixler, Louis A. Altamura, Catherine Arnold, William D. Pratt , et al. · 2018

An animal model of Ebola virus infection with variable disease onset identifies host markers of infection that precede symptoms by 4 days.

Zika Virus Infection in Syrian Golden Hamsters and Strain 13 Guinea Pigs Open

Lynn J. Miller, Farooq Nasar, Christopher W. Schellhase, Sarah L. Norris, Adrienne E. Kimmel , et al. · 2018

To evaluate potential immunocompetent small animal models of Zika virus (ZIKV) infection, we inoculated Syrian golden hamsters (subcutaneously or intraperitoneally) and strain 13 guinea pigs (intraperitoneally) with Senegalese ZIKV strain …

DNA vaccines elicit durable protective immunity against individual or simultaneous infections with Lassa and Ebola viruses in guinea pigs Open

Kathleen A. Cashman, Eric R. Wilkinson, Suzanne E. Wollen-Roberts, Joshua D. Shamblin, Justine M. Zelko , et al. · 2017

We previously developed optimized DNA vaccines against both Lassa fever and Ebola hemorrhagic fever viruses and demonstrated that they were protective individually in guinea pig and nonhuman primate models. In this study, we vaccinated gro…

A DNA vaccine delivered by dermal electroporation fully protects cynomolgus macaques against Lassa fever Open

Kathleen A. Cashman, Eric R. Wilkinson, Carl Shaia, Paul R. Facemire, Todd M. Bell , et al. · 2017

Lassa virus (LASV) is an ambisense RNA virus in the Arenaviridae family and is the etiological agent of Lassa fever, a severe hemorrhagic disease endemic to West and Central Africa. ^1,2 There are no US Food and Drug Administrati…

High Infection Rates for Adult Macaques after Intravaginal or Intrarectal Inoculation with Zika Virus Open

Andrew D. Haddow, Ayşegül Nalça, Franco Rossi, Lynn J. Miller, Michael R. Wiley , et al. · 2017

Unprotected sexual intercourse between persons residing in or traveling from regions with Zika virus transmission is a risk factor for infection. To model risk for infection after sexual intercourse, we inoculated rhesus and cynomolgus mac…

Low potential for mechanical transmission of Ebola virus via house flies (Musca domestica) Open

Andrew D. Haddow, Farooq Nasar, Christopher W. Schellhase, Roger D. Moon, Susana Padilla , et al. · 2017

Isolation of potent neutralizing antibodies from a survivor of the 2014 Ebola virus outbreak Open

Zachary A. Bornholdt, Hannah L. Turner, Charles D. Murin, Wen Li, Devin Sok , et al. · 2016

Profiling the antibody response to Ebola The recent Ebola virus outbreak in West Africa illustrates the need not only for a vaccine but for potential therapies, too. One promising therapy is monoclonal antibodies that target Ebola's membra…

Ebola Virus Infections in Nonhuman Primates Are Temporally Influenced by Glycoprotein Poly-U Editing Site Populations in the Exposure Material Open

John C. Trefry, Suzanne E. Wollen-Roberts, Farooq Nasar, Joshua D. Shamblin, Steven J. Kern , et al. · 2015

Recent experimentation with the variants of the Ebola virus that differ in the glycoprotein’s poly-uridine site, which dictates the form of glycoprotein produced through a transcriptional stutter, has resulted in questions regarding the pa…

Dose Response of MARV/Angola Infection in Cynomolgus Macaques following IM or Aerosol Exposure Open

Sara C. Johnston, Kenny Lin, Nancy Twenhafel, Jo Lynne Raymond, Joshua D. Shamblin , et al. · 2015

Marburg virus infection in humans causes a hemorrhagic disease with a high case fatality rate. Countermeasure development requires the use of well-characterized animal models that mimic human disease. To further characterize the cynomolgus…

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