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View article: Parp7 generates an ADP-ribosyl degron that controls negative feedback of androgen signaling
Parp7 generates an ADP-ribosyl degron that controls negative feedback of androgen signaling Open
The androgen receptor (AR) transduces the effects of circulating and tumor-derived androgens to the nucleus through ligand-induced changes in protein conformation, localization, and chromatin engagement. Defining how these events are integ…
View article: Convergent Approach Toward ADP‐Ribosylated Peptides via a Chemoselective Phosphate Condensation
Convergent Approach Toward ADP‐Ribosylated Peptides via a Chemoselective Phosphate Condensation Open
The preparation of well‐defined ADP‐ribosylated peptides is essential for studying the functional implications of this post‐translational modification. While methodologies exist for the chemical synthesis of short oligo‐ADPr fragments and …
View article: Family-wide analysis of human macrodomains reveals novel activities and identifies PARG as most efficient ADPr-RNA hydrolase
Family-wide analysis of human macrodomains reveals novel activities and identifies PARG as most efficient ADPr-RNA hydrolase Open
View article: Parp7 generates an ADP-ribosyl degron that controls negative feedback of androgen signaling
Parp7 generates an ADP-ribosyl degron that controls negative feedback of androgen signaling Open
The androgen receptor (AR) tranduces the effects of circulating and tumor-derived androgens to the nucleus through ligand-induced changes in protein conformation, localization, and engagement with chromatin binding sites. Understanding the…
View article: Reversal of tyrosine-linked ADP-ribosylation by ARH3 and PARG
Reversal of tyrosine-linked ADP-ribosylation by ARH3 and PARG Open
ADP-ribosylation is an ancient posttranslational modification with exceptional versatility in terms of breadth of modification targets including at least seven different amino acid side chains, various moieties on nucleic acids, and a vari…
View article: Family-wide analysis of human macrodomains reveals novel activities
Family-wide analysis of human macrodomains reveals novel activities Open
ADP-ribosylation is well-known as protein posttranslational modification and was recently also identified as RNA posttranscriptional modification. ADP-ribose is added onto substrates by PARP enzymes and removed by the structurally distinct…
View article: Synthesis of Structural ADP-Ribose Analogues as Inhibitors for SARS-CoV-2 Macrodomain 1
Synthesis of Structural ADP-Ribose Analogues as Inhibitors for SARS-CoV-2 Macrodomain 1 Open
Protein adenosine diphosphate (ADP)-ribosylation is crucial for a proper immune response. Accordingly, viruses have evolved ADP-ribosyl hydrolases to remove these modifications, a prominent example being the SARS-CoV-2 NSP3 macrodomain, "M…
View article: Synthetic Dual Cysteine-ADP Ribosylated Peptides from the Androgen Receptor are Recognized by the DTX3L/PARP9 Complex
Synthetic Dual Cysteine-ADP Ribosylated Peptides from the Androgen Receptor are Recognized by the DTX3L/PARP9 Complex Open
Androgen signaling in prostate cancer cells involves multisite cysteine ADP-ribosylation of the androgen receptor (AR) by PARP7. The AR modification is read by ADP-ribosyl binding macrodomains in PARP9, but the reason that multiple cystein…
View article: PARP14 is a PARP with both ADP-ribosyl transferase and hydrolase activities
PARP14 is a PARP with both ADP-ribosyl transferase and hydrolase activities Open
PARP14 is a mono–ADP-ribosyl transferase involved in the control of immunity, transcription, and DNA replication stress management. However, little is known about the ADP-ribosylation activity of PARP14, including its substrate specificity…
View article: PARP14 is a PARP with both ADP-ribosyl transferase and hydrolase activities
PARP14 is a PARP with both ADP-ribosyl transferase and hydrolase activities Open
PARP14 is a mono-ADP-ribosyl transferase involved in the control of immunity, transcription and DNA replication stress management. However, little is known about the ADP-ribosylation activity of PARP14, including its substrate specificity …
View article: Chemoenzymatic and Synthetic Approaches To Investigate Aspartate- and Glutamate-ADP-Ribosylation
Chemoenzymatic and Synthetic Approaches To Investigate Aspartate- and Glutamate-ADP-Ribosylation Open
We report here chemoenzymatic and fully synthetic methodologies to modify aspartate and glutamate side chains with ADP-ribose at specific sites on peptides. Structural analysis of aspartate and glutamate ADP-ribosylated peptides reveals ne…
View article: Arginine ADP-Ribosylation: Chemical Synthesis of Post-Translationally Modified Ubiquitin Proteins
Arginine ADP-Ribosylation: Chemical Synthesis of Post-Translationally Modified Ubiquitin Proteins Open
We describe the development and optimization of a methodology to prepare peptides and proteins modified on the arginine residue with an adenosine-di-phosphate-ribosyl (ADPr) group. Our method comprises reacting an ornithine containing poly…
View article: Heterogeneous Catalytic Oxidation of Simple Alcohols by Transition Metals
Heterogeneous Catalytic Oxidation of Simple Alcohols by Transition Metals Open
The "exploding" flask demonstration presents a well-known illustration of heterogeneous catalyzed methanol oxidation. We find that for the same vapor pressure, the demonstration also works for all primary and secondary alcohols up to butan…