Sylvain Pastezeur
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Kinesin-12 KLP-18 contributes to the kinetochore-microtubule poleward flux during the metaphase of C. elegans one-cell embryo. Open
The mitotic spindle partitions chromosomes during cell division by connecting the poles to kinetochores through microtubules (MTs). Their plus-ends, facing the chromosomes, exhibit dynamic instability, which is critical for proper attachme…
Microtubule stiffening by the doublecortin-domain protein ZYG-8 contributes to mitotic spindle orientation during zygote division in <i>Caenorhabditis elegans</i> Open
In Caenorhabditis elegans , mutations in zyg-8 DCLK1 , the sole Doublecortin-family member, led to incorrect mitotic spindle positioning, as seen by immunofluorescence. Doublecortin proteins bind to microtubules and are thought to stabilis…
Unveiling inter-embryo variability in spindle length over time: Towards quantitative phenotype analysis Open
How can inter-individual variability be quantified? Measuring many features per experiment raises the question of choosing them to recapitulate high-dimensional data. Tackling this challenge on spindle elongation phenotypes, we showed that…
Only three principal components account for inter-embryo variability of the spindle length over time Open
How does inter-individual variability emerge? When measuring a large number of features per experiment/individual, this question becomes non-trivial. One challenge lies in choosing features to recapitulate high-dimension data. In this pape…
Kinesin-12 KLP-18 contributes to the kinetochore-microtubule poleward flux during the metaphase of <i>C. elegans</i> one-cell embryo Open
The mitotic spindle, a key structure to partition chromosomes during cell division, connects its poles to the chromosomes through microtubules. Their plus-ends, oriented towards the chromosomes, exhibit dynamic instability crucial for kine…
The coordination of spindle-positioning forces during the asymmetric division of the <i>C. elegans</i> zygote is revealed by distinct microtubule dynamics at the cortex Open
In the Caenorhabditis elegans zygote, astral microtubules generate forces, pushing against and pulling from the cell periphery. They are essential to position the mitotic spindle. By measuring the dynamics of astral microtubules at the cor…
The polarity-induced force imbalance in<i>Caenorhabditis elegans</i>embryos is caused by asymmetric binding rates of dynein to the cortex Open
During asymmetric cell division, the molecular motor dynein generates cortical pulling forces that position the spindle to reflect polarity and adequately distribute cell fate determinants. In Caenorhabditis elegans embryos, despite a meas…
Dynamics of dynein at microtubule plus-ends and the cortex during the division of the C. elegans zygote Open
During asymmetric cell divisions, cortical dyneins generate forces essential to position the spindle after polarity cues, prescribing daughter cells fate. In nematode zygote, cortical dynein pulls on microtubules transiently, raising the q…
Dynein dynamics at the microtubule plus-ends and cortex during division in the C. $\textit{elegans}$ zygote Open
During asymmetric cell division, dynein generates forces, which position the spindle to reflect polarity and ensure correct daughter cell fates. The transient cortical localization of dynein raises the question of its targeting. We found t…
Dynein dynamics at the microtubule plus-ends and cortex during division in the <i>C. elegans</i> zygote Open
During asymmetric cell division, dynein generates forces, which position the spindle to reflect polarity and ensure correct daughter cell fates. The transient cortical localization of dynein raises the question of its targeting. We found t…
LET-99-dependent spatial restriction of active force generators makes spindle's position robust Open
During the asymmetric division of the Caenorhabditis elegans nematode zygote, the polarity cues distribution and daughter cell fates depend on the correct positioning of the mitotic spindle, which results from both centering and cortical p…
The mitotic spindle in the one-cell <i>C. elegans</i> embryo is positioned with high precision and stability Open
Precise positioning of the mitotic spindle is important for specifying the plane of cell division, which in turn determines how the cytoplasmic contents of the mother cell are partitioned into the daughter cells, and how the daughters are …