Tadayuki Imanaka
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View article: Direct Fixation of Atmospheric CO2 towards Chemical Synthesis of Fuel Hydrocarbon
Direct Fixation of Atmospheric CO2 towards Chemical Synthesis of Fuel Hydrocarbon Open
We have reported that fuel hydrocarbon was chemically synthesized from CO2 and activated water. In this report, we show the direct evidence of CO2 fixation from air. CO2 in the air is composed of about 99 % 12C, about 1% 13C, and about one…
View article: Chemical Synthesis of Fuel Hydrocarbon from CO2 and Activated Water, and Purification of Commercial Light Oil for Dream Oil
Chemical Synthesis of Fuel Hydrocarbon from CO2 and Activated Water, and Purification of Commercial Light Oil for Dream Oil Open
Here we show that petroleum can be formed efficiently at normal temperatures and pressures from carbon dioxide and activated water. The CO2 nano-bubble containing water was treated with photocatalyst in the presence of oxygen under UV irra…
View article: The C‐terminal flexible region of branched‐chain polyamine synthase facilitates substrate specificity and catalysis
The C‐terminal flexible region of branched‐chain polyamine synthase facilitates substrate specificity and catalysis Open
Branched‐chain polyamine synthase (BpsA) catalyzes sequential aminopropyl transfer from the donor, decarboxylated S ‐adenosylmethionine (dc SAM ), to the acceptor, linear‐chain polyamine, resulting in the production of a quaternary‐branche…
View article: Pol B, a Family B DNA Polymerase, in <i>Thermococcus kodakarensis</i> is Important for DNA Repair, but not DNA Replication
Pol B, a Family B DNA Polymerase, in <i>Thermococcus kodakarensis</i> is Important for DNA Repair, but not DNA Replication Open
Thermococcus kodakarensis possesses two DNA polymerases, Pol B and Pol D. We generated a T. kodakarensis strain (DPB1) in which polB was completely deleted and a derivative of DPB1 in which polB was overexpressed; neither of the generated …
View article: Random mutagenesis of a hyperthermophilic archaeon identified tRNA modifications associated with cellular hyperthermotolerance
Random mutagenesis of a hyperthermophilic archaeon identified tRNA modifications associated with cellular hyperthermotolerance Open
Random mutagenesis for the hyperthermophilic archaeon Thermococcus kodakarensis was established by the insertion of an artificial transposon designed to allow easy identification of the transposon-inserted locus. The phenotypic screening w…
View article: Front Cover: Branched‐Chain Polyamine Found in Hyperthermophiles Induces Unique Temperature‐Dependent Structural Changes in Genome‐Size DNA (ChemPhysChem 18/2018)
Front Cover: Branched‐Chain Polyamine Found in Hyperthermophiles Induces Unique Temperature‐Dependent Structural Changes in Genome‐Size DNA (ChemPhysChem 18/2018) Open
The Front Cover shows generation of multiple nano-loop structures along a strand of large DNA at 80°C in the presence of a unique branched-chain polyamine found in a hyperthermophilic archaeon. More information can be found in the Article …
View article: Branched‐Chain Polyamine Found in Hyperthermophiles Induces Unique Temperature‐Dependent Structural Changes in Genome‐Size DNA
Branched‐Chain Polyamine Found in Hyperthermophiles Induces Unique Temperature‐Dependent Structural Changes in Genome‐Size DNA Open
A pentavalent branched‐chain polyamine, N 4 ‐bis(aminopropyl)spermidine 3(3)(3)4, is a unique polycation found in the hyperthermophilic archaeon Thermococcus kodakarensis , which grows at temperatures between 60 and 100 °C. We studied the …
View article: An ornithine ω-aminotransferase required for growth in the absence of exogenous proline in the archaeon Thermococcus kodakarensis
An ornithine ω-aminotransferase required for growth in the absence of exogenous proline in the archaeon Thermococcus kodakarensis Open
View article: Active site geometry of a novel aminopropyltransferase for biosynthesis of hyperthermophile‐specific branched‐chain polyamine
Active site geometry of a novel aminopropyltransferase for biosynthesis of hyperthermophile‐specific branched‐chain polyamine Open
Branched‐chain polyamines are found exclusively in thermophilic bacteria and Euryarchaeota and play essential roles in survival at high temperatures. In the present study, kinetic analyses of a branched‐chain polyamine synthase from the hy…
View article: A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and γ-ray-irradiation, and freezing
A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and γ-ray-irradiation, and freezing Open
Dimethyl sulfoxide (DMSO) is widely used as a cryoprotectant for organs, tissues, and cell suspension in storage. In addition, DMSO is known to be a useful free radical scavenger and a radio-protectant. To date, many in vitro assays using …
View article: Anthranilate phosphoribosyltransferase from the hyperthermophilic archaeon <i>Thermococcus kodakarensis</i> shows maximum activity with zinc and forms a unique dimeric structure
Anthranilate phosphoribosyltransferase from the hyperthermophilic archaeon <i>Thermococcus kodakarensis</i> shows maximum activity with zinc and forms a unique dimeric structure Open
Anthranilate phosphoribosyltransferase (TrpD) is involved in tryptophan biosynthesis, catalyzing the transfer of a phosphoribosyl group to anthranilate, leading to the generation of phosphoribosyl anthranilate. TrpD belongs to the phosphor…
View article: Engineering of the Hyperthermophilic Archaeon Thermococcus kodakarensis for Chitin-Dependent Hydrogen Production
Engineering of the Hyperthermophilic Archaeon Thermococcus kodakarensis for Chitin-Dependent Hydrogen Production Open
Thermococcus kodakarensis is a hyperthermophilic archaeon that harbors a complete set of genes for chitin degradation to fructose 6-phosphate. However, wild-type T. kodakarensis KOD1 does not display growth on chitin. In this study, we dev…
View article: Double‐Strand Breaks in Genome‐Sized DNA Caused by Ultrasound
Double‐Strand Breaks in Genome‐Sized DNA Caused by Ultrasound Open
DNA double‐strand breaks (DSBs) caused by ultrasound were evaluated in a quantitative manner by single‐molecule fluorescence microscopy. We compared the effect of time‐interval (or pulse) sonication to that of continuous wave (CW) sonicati…
View article: Naturally occurring branched-chain polyamines induce a crosslinked meshwork structure in a giant DNA
Naturally occurring branched-chain polyamines induce a crosslinked meshwork structure in a giant DNA Open
We studied the effect of branched-chain polyamines on the folding transition of genome-sized DNA molecules in aqueous solution by the use of single-molecule observation with fluorescence microcopy. Detailed morphological features of polyam…
View article: An archaeal ADP-dependent serine kinase involved in cysteine biosynthesis and serine metabolism
An archaeal ADP-dependent serine kinase involved in cysteine biosynthesis and serine metabolism Open
View article: Metabolic engineering of oleaginous yeast Yarrowia lipolytica for limonene overproduction
Metabolic engineering of oleaginous yeast Yarrowia lipolytica for limonene overproduction Open
This is the first report on limonene biosynthesis in oleaginous yeast Y. lipolytica by heterologous expression of codon-optimized tLS and tNDPS1 genes. To our knowledge, the limonene production 23.56 mg/L, is the highe…
View article: Erratum to: Pcal_0768, a hyperactive 4-α-glucanotransferase from Pyrobaculum calidifontis
Erratum to: Pcal_0768, a hyperactive 4-α-glucanotransferase from Pyrobaculum calidifontis Open
View article: MOESM1 of Metabolic engineering of oleaginous yeast Yarrowia lipolytica for limonene overproduction
MOESM1 of Metabolic engineering of oleaginous yeast Yarrowia lipolytica for limonene overproduction Open
Additional file 1. The optimized nucleotide sequences of truncated LS gene and truncated NDPS1 gene.
View article: Highly Concentrated Ethanol Solutions: Good Solvents for DNA as Revealed by Single‐Molecule Observation
Highly Concentrated Ethanol Solutions: Good Solvents for DNA as Revealed by Single‐Molecule Observation Open
We observed single DNA molecules at different ethanol concentrations by using fluorescence microscopy. Large single DNA molecules undergo reentrant conformational transitions from elongated coil into folded globule and then into elongated …
View article: [Retracted] An efficient way of producing fuel hydrocarbon from CO<sub>2</sub> and activated water
[Retracted] An efficient way of producing fuel hydrocarbon from CO<sub>2</sub> and activated water Open
View article: Protective effect of ascorbic acid against double-strand breaks in giant DNA: Marked differences among the damage induced by photo-irradiation, gamma-rays and ultrasound
Protective effect of ascorbic acid against double-strand breaks in giant DNA: Marked differences among the damage induced by photo-irradiation, gamma-rays and ultrasound Open
The protective effect of ascorbic acid against double-strand breaks in DNA was evaluated by single-molecule observation of giant DNA (T4 DNA; 166 kbp) through fluorescence microscopy. Samples were exposed to three different forms of radiat…
View article: Overproduction of the membrane-bound [NiFe]-hydrogenase in Thermococcus kodakarensis and its effect on hydrogen production
Overproduction of the membrane-bound [NiFe]-hydrogenase in Thermococcus kodakarensis and its effect on hydrogen production Open
The hyperthermophilic archaeon Thermococcus kodakarensis can utilize sugars or pyruvate for growth. In the absence of elemental sulfur, the electrons via oxidation of these substrates are accepted by protons, generating molecular hydrogen …
View article: Structural basis of a Ni acquisition cycle for [NiFe] hydrogenase by Ni-metallochaperone HypA and its enhancer
Structural basis of a Ni acquisition cycle for [NiFe] hydrogenase by Ni-metallochaperone HypA and its enhancer Open
Significance The metal ions in proteins are correctly incorporated by specific metallochaperones. However, it remains unclear how metallochaperones regulate their metal binding affinity during acquisition of correct metal ions and deliver …
View article: A Mutant Chaperonin That Is Functional at Lower Temperatures Enables Hyperthermophilic Archaea To Grow under Cold-Stress Conditions
A Mutant Chaperonin That Is Functional at Lower Temperatures Enables Hyperthermophilic Archaea To Grow under Cold-Stress Conditions Open
Thermococcus kodakarensis grows optimally at 85°C and possesses two chaperonins, cold-inducible CpkA and heat-inducible CpkB, which are involved in adaptation to low and high temperatures, respectively. The two chaperonins share a high seq…
View article: Characterization of a thermostable glucose dehydrogenase with strict substrate specificity from a hyperthermophilic archaeon <i>Thermoproteus</i> sp. GDH-1
Characterization of a thermostable glucose dehydrogenase with strict substrate specificity from a hyperthermophilic archaeon <i>Thermoproteus</i> sp. GDH-1 Open
A hyperthermophilic archaeon was isolated from a terrestrial hot spring on Kodakara Island, Japan and designated as Thermoproteus sp. glucose dehydrogenase (GDH-1). Cell extracts from cells grown in medium supplemented with glucose exhibit…