Thean Chor Leow
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View article: Methanol response of V171S lipase from Geobacillus sp. 42 explored via experimental and in silico approaches
Methanol response of V171S lipase from Geobacillus sp. 42 explored via experimental and in silico approaches Open
The stability of lipase in organic solvents is crucial for biocatalytic processes in industrial biotechnology. Previously, we described a thermostable and solvent-tolerant Lip 42 from Geobacillus sp. Despite these features, the wild-type L…
View article: Exploring the impacts of terminal mutations on the thermostability of Bacillus sp. L2 lipase
Exploring the impacts of terminal mutations on the thermostability of Bacillus sp. L2 lipase Open
Protein engineering has been widely used to improve enzyme properties and make them appropriate for use as industrial biocatalysts. To study the effect of mutation at the N- and C-terminal of lipase, two double mutants (A8V/S385E and A8P/S…
View article: 16S rRNA metagenomics data on the bacterial communities in Poring Hot Spring, Sabah, Malaysia
16S rRNA metagenomics data on the bacterial communities in Poring Hot Spring, Sabah, Malaysia Open
Hot springs are known to harbor potentially unique microorganisms due to the extreme temperatures in which they thrive and their biotechnologically important enzymes that are active at high temperature, which are beneficial for various ind…
View article: Plasmid-based and genome-based expression of recombinant T1 lipase in sucrose-utilizing E. coli strain W
Plasmid-based and genome-based expression of recombinant T1 lipase in sucrose-utilizing E. coli strain W Open
Given its thermoalkaliphilic properties, T1 lipase holds significant potential for diverse industrial applications. However, traditional expression methods in Escherichia coli, specifically the plasmid-based system, present challenges of e…
View article: Sequence, structure and biophysical characterization of CsoR-like hypothetical protein from Geobacillus zalihae strain T1
Sequence, structure and biophysical characterization of CsoR-like hypothetical protein from Geobacillus zalihae strain T1 Open
Aims:To date, nine classes of copper regulation mechanisms have been discovered in bacteria and CsoR regulator protein is the most recent among them.Only a few have been structurally and functionally characterized.The present study was aim…
View article: WITHDRAWN: Structural analysis, molecular dynamics simulation and thermodynamic modification of the antifreeze protein type IV mutant under subfreezing temperatures
WITHDRAWN: Structural analysis, molecular dynamics simulation and thermodynamic modification of the antifreeze protein type IV mutant under subfreezing temperatures Open
Antifreeze proteins (AFPs) are expressed by numerous organisms for their survivability in polar regions due to their special functions; ice recrystallization inhibition (IRI) and thermal hysteresis (TH). Nevertheless, the inherent employme…
View article: Heterologous expression and purification of calmodulin from Plasmodium knowlesi using codon-optimized synthetic gene
Heterologous expression and purification of calmodulin from Plasmodium knowlesi using codon-optimized synthetic gene Open
Aims: Calmodulin (CaM) is vital for the survival of Plasmodium knowlesi, a simian malaria parasite that infects both macaques and humans in Southeast Asia.To advance antimalarial drugs development targeting this protein, it is imperative t…
View article: Inhibition of SARS-CoV-2 3CL protease by the anti-viral chimeric protein RetroMAD1
Inhibition of SARS-CoV-2 3CL protease by the anti-viral chimeric protein RetroMAD1 Open
COVID-19 results from SARS-CoV-2, which mutates frequently, challenging current treatments. Therefore, it is critical to develop new therapeutic drugs against this disease. This study explores the interaction between SARS-CoV-2 3CL pro and…
View article: Anti-viral chimeric protein RetroMAD1™ potently block SARS-CoV-2 viral entry and propagation
Anti-viral chimeric protein RetroMAD1™ potently block SARS-CoV-2 viral entry and propagation Open
COVID-19 is a disease caused by the highly transmissible and pathogenic SARS-CoV-2 virus. Since its first case was documented in 2019, it has rapidly widespread and has caused millions of deaths worldwide. Many intervention strategies targ…
View article: Altering the Regioselectivity of T1 Lipase from Geobacillus zalihae toward sn-3 Acylglycerol Using a Rational Design Approach
Altering the Regioselectivity of T1 Lipase from Geobacillus zalihae toward sn-3 Acylglycerol Using a Rational Design Approach Open
The regioselectivity characteristic of lipases facilitate a wide range of novel molecule unit constructions and fat modifications. Lipases can be categorized as sn-1,3, sn-2, and random regiospecific. Geobacillus zalihae T1 lipase catalyze…
View article: Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues
Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues Open
Cold environments characterised by diverse temperatures close to or below the water freezing point dominate about 80% of the Earth’s biosphere. One of the survival strategies adopted by microorganisms living in cold environments is their e…
View article: Crosstalk between Fatty Acid Metabolism and Tumour-Associated Macrophages in Cancer Progression
Crosstalk between Fatty Acid Metabolism and Tumour-Associated Macrophages in Cancer Progression Open
Over the last few decades, cancer has been regarded as an independent and self sustaining progression. The earliest hallmarks of cancer comprise of sustaining proliferative signalling, avoiding growth suppressors, resisting cell death, ena…
View article: CD36-Fatty Acid-Mediated Metastasis via the Bidirectional Interactions of Cancer Cells and Macrophages
CD36-Fatty Acid-Mediated Metastasis via the Bidirectional Interactions of Cancer Cells and Macrophages Open
Tumour heterogeneity refers to the complexity of cell subpopulations coexisting within the tumour microenvironment (TME), such as proliferating tumour cells, tumour stromal cells and infiltrating immune cells. The bidirectional interaction…
View article: Clinical and Preclinical Studies of Fermented Foods and Their Effects on Alzheimer’s Disease
Clinical and Preclinical Studies of Fermented Foods and Their Effects on Alzheimer’s Disease Open
The focus on managing Alzheimer’s disease (AD) is shifting towards prevention through lifestyle modification instead of treatments since the currently available treatment options are only capable of providing symptomatic relief marginally …
View article: <i>In silico</i> identification and characterization of potential druggable targets among hypothetical proteins of <i>Leptospira interrogans</i> serovar Copenhageni: a comprehensive bioinformatics approach
<i>In silico</i> identification and characterization of potential druggable targets among hypothetical proteins of <i>Leptospira interrogans</i> serovar Copenhageni: a comprehensive bioinformatics approach Open
Leptospirosis is one of the neglected zoonosis, affecting human and animal populations worldwide. Reliable effective therapeutics and concerns to look for more research into the molecular analysis of its genome is therefore needed. In the …
View article: Selected Kefir Water from Malaysia Attenuates Hydrogen Peroxide-Induced Oxidative Stress by Upregulating Endogenous Antioxidant Levels in SH-SY5Y Neuroblastoma Cells
Selected Kefir Water from Malaysia Attenuates Hydrogen Peroxide-Induced Oxidative Stress by Upregulating Endogenous Antioxidant Levels in SH-SY5Y Neuroblastoma Cells Open
Kefir, a fermented probiotic drink was tested for its potential anti-oxidative, anti-apoptotic, and neuroprotective effects to attenuate cellular oxidative stress on human SH-SY5Y neuroblastoma cells. Here, the antioxidant potentials of th…
View article: Structure elucidation and docking analysis of 5M mutant of T1 lipase Geobacillus zalihae
Structure elucidation and docking analysis of 5M mutant of T1 lipase Geobacillus zalihae Open
5M mutant lipase was derived through cumulative mutagenesis of amino acid residues (D43E/T118N/E226D/E250L/N304E) of T1 lipase from Geobacillus zalihae . A previous study revealed that cumulative mutations in 5M mutant lipase resulted in d…
View article: REASSESSMENT OF THE CATALYTIC ACTIVITY AND SUBSTRATE SPECIFICITY OF FKBP35 FROM Plasmodium knowlesi USING PROTEASE-FREE ASSAY
REASSESSMENT OF THE CATALYTIC ACTIVITY AND SUBSTRATE SPECIFICITY OF FKBP35 FROM Plasmodium knowlesi USING PROTEASE-FREE ASSAY Open
FK506-binding protein35 of Plasmodium knowlesi (Pk-FKBP35) is a member of peptidyl prolyl cis-trans isomerase (PPIase) and is considered as a promising avenue of antimalarial drug target development. This protein is organized into the N-te…
View article: Design and Characterisation of Inhibitory Peptides against Bleg1_2478, an Evolutionary Divergent B3 Metallo-β-lactamase
Design and Characterisation of Inhibitory Peptides against Bleg1_2478, an Evolutionary Divergent B3 Metallo-β-lactamase Open
Previously, a hypothetical protein (HP) termed Bleg1_2437 (currently named Bleg1_2478) from Bacillus lehensis G1 was discovered to be an evolutionary divergent B3 subclass metallo-β-lactamase (MBL). Due to the scarcity of clinical inhibito…
View article: Antifreeze Proteins and Their Practical Utilization in Industry, Medicine, and Agriculture
Antifreeze Proteins and Their Practical Utilization in Industry, Medicine, and Agriculture Open
Antifreeze proteins (AFPs) are specific proteins, glycopeptides, and peptides made by different organisms to allow cells to survive in sub-zero conditions. AFPs function by reducing the water’s freezing point and avoiding ice crystals’ gro…
View article: Identification of potential riboflavin synthase inhibitors by virtual screening and molecular dynamics simulation studies
Identification of potential riboflavin synthase inhibitors by virtual screening and molecular dynamics simulation studies Open
Riboflavin synthase is an important enzyme catalyzing the last step of riboflavin biosynthesis in microorganisms. Due to the absolute dependency of the microbes to this biosynthetic pathway coupled with its nonexistence in humans, riboflav…
View article: A Host-Vector System for the Expression of a Thermostable Bacterial Lipase in a Locally Isolated Meyerozyma guilliermondii SMB
A Host-Vector System for the Expression of a Thermostable Bacterial Lipase in a Locally Isolated Meyerozyma guilliermondii SMB Open
Screening for a new yeast as an alternative host is expected to solve the limitations in the present yeast expression system. A yeast sample which was isolated from the traditional food starter ‘ragi’ from Malaysia was identified to contai…
View article: Effective refolding of a cysteine rich glycoside hydrolase family 19 recombinant chitinase from Streptomyces griseus by reverse dilution and affinity chromatography
Effective refolding of a cysteine rich glycoside hydrolase family 19 recombinant chitinase from Streptomyces griseus by reverse dilution and affinity chromatography Open
Conventional refolding methods are associated with low yields due to misfolding and high aggregation rates or very dilute proteins. In this study, we describe the optimization of the conventional methods of reverse dilution and affinity ch…
View article: The Influence of Calcium toward Order/Disorder Conformation of Repeat-in-Toxin (RTX) Structure of Family I.3 Lipase from Pseudomonas fluorescens AMS8
The Influence of Calcium toward Order/Disorder Conformation of Repeat-in-Toxin (RTX) Structure of Family I.3 Lipase from Pseudomonas fluorescens AMS8 Open
Calcium-binding plays a decisive role in the folding and stabilization of many RTX proteins, especially for the RTX domain. Although many studies have been conducted to prove the contribution of Ca2+ ion toward the folding and stabilizatio…
View article: The Role of Surface Exposed Lysine in Conformational Stability and Functional Properties of Lipase from Staphylococcus Family
The Role of Surface Exposed Lysine in Conformational Stability and Functional Properties of Lipase from Staphylococcus Family Open
Surface charge residues have been recognized as one of the stability determinants in protein. In this study, we sought to compare and analyse the stability and conformational dynamics of staphylococcal lipase mutants with surface lysine mu…