Takuma Kishimoto
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View article: Double prenylation of budding yeast Ykt6 regulates cell wall integrity and autophagy
Double prenylation of budding yeast Ykt6 regulates cell wall integrity and autophagy Open
View article: A non-toxic equinatoxin-II reveals the dynamics and distribution of sphingomyelin in the cytosolic leaflet of the plasma membrane
A non-toxic equinatoxin-II reveals the dynamics and distribution of sphingomyelin in the cytosolic leaflet of the plasma membrane Open
Sphingomyelin (SM) is a major sphingolipid in mammalian cells. SM is enriched in the extracellular leaflet of the plasma membrane (PM). Besides this localization, recent electron microscopic and biochemical studies suggest the presence of …
View article: Inhibition Mechanism of SARS-CoV-2 Infection by a Cholesterol Derivative, Nat-20(S)-yne
Inhibition Mechanism of SARS-CoV-2 Infection by a Cholesterol Derivative, Nat-20(S)-yne Open
The coronavirus disease 2019 (COVID-19) is caused by the etiological agent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). COVID-19, with the recurrent epidemics of new variants of SARS-CoV-2, remains a global public health p…
View article: Single-molecule localization microscopy reveals STING clustering at the trans-Golgi network through palmitoylation-dependent accumulation of cholesterol
Single-molecule localization microscopy reveals STING clustering at the trans-Golgi network through palmitoylation-dependent accumulation of cholesterol Open
Stimulator of interferon genes (STING) is critical for the type I interferon response to pathogen- or self-derived DNA in the cytosol. STING may function as a scaffold to activate TANK-binding kinase 1 (TBK1), but direct cellular evidence …
View article: A non-toxic equinatoxin-II reveals the dynamics of sphingomyelin in the cytosolic leaflet of the plasma membrane
A non-toxic equinatoxin-II reveals the dynamics of sphingomyelin in the cytosolic leaflet of the plasma membrane Open
Summary Super-resolution microscopic observation of a novel non-toxic sphingomyelin probe revealed the formation of dynamic small domains including sphingomyelin and cholesterol in the cytosolic leaflet of living cell plasma membranes. Abs…
View article: Plasma membrane phosphatidylinositol (4,5)-bisphosphate is critical for determination of epithelial characteristics
Plasma membrane phosphatidylinositol (4,5)-bisphosphate is critical for determination of epithelial characteristics Open
View article: Phospholipid flippases and Sfk1 are essential for the retention of ergosterol in the plasma membrane
Phospholipid flippases and Sfk1 are essential for the retention of ergosterol in the plasma membrane Open
Phospholipids are asymmetrically distributed in the plasma membrane (PM). However, our understanding of their physiological significance is still limited. Here we show that phospholipid flippases and a PM protein, Sfk1, cooperatively play …
View article: Phospholipid flippases and Sfk1 are essential for the retention of ergosterol in the plasma membrane
Phospholipid flippases and Sfk1 are essential for the retention of ergosterol in the plasma membrane Open
Sterols are important lipid components of the plasma membrane (PM) in eukaryotic cells, but it is unknown how the PM retains sterols at a high concentration. Phospholipids are asymmetrically distributed in the PM, and phospholipid flippase…
View article: Phosphatidylserine prevents the generation of a protein-free giant plasma membrane domain in yeast
Phosphatidylserine prevents the generation of a protein-free giant plasma membrane domain in yeast Open
Membrane phase separation accompanied with micron-scale domains of lipids and proteins occurs in artificial membranes; however, a similar large phase separation has not been reported in the plasma membrane of the living cells. We demonstra…
View article: A complex genetic interaction implicates that phospholipid asymmetry and phosphate homeostasis regulate Golgi functions
A complex genetic interaction implicates that phospholipid asymmetry and phosphate homeostasis regulate Golgi functions Open
In eukaryotic cells, phospholipid flippases translocate phospholipids from the exoplasmic to the cytoplasmic leaflet of the lipid bilayer. Budding yeast contains five flippases, of which Cdc50p-Drs2p and Neo1p are primarily involved in mem…
View article: Issue Information
Issue Information Open
Long non-coding RNA LINC00337 induces autophagy and chemoresistance to cisplatin
View article: Cholesterol asymmetry at the tip of filopodia during cell adhesion
Cholesterol asymmetry at the tip of filopodia during cell adhesion Open
During adhesion, cells develop filopodia to facilitate the attachment to the extracellular matrix. The small guanosine triphosphate (GTP)‐binding protein, Cdc42, plays a central role in the formation of filopodia. It has been reported that…
View article: Phospholipid flippases and Sfk1p, a novel regulator of phospholipid asymmetry, contribute to low permeability of the plasma membrane
Phospholipid flippases and Sfk1p, a novel regulator of phospholipid asymmetry, contribute to low permeability of the plasma membrane Open
Phospholipid flippase (type 4 P-type ATPase) plays a major role in the generation of phospholipid asymmetry in eukaryotic cell membranes. Loss of Lem3p-Dnf1/2p flippases leads to the exposure of phosphatidylserine (PS) and phosphatidyletha…
View article: A novel sphingomyelin/cholesterol domain‐specific probe reveals the dynamics of the membrane domains during virus release and in Niemann‐Pick type C
A novel sphingomyelin/cholesterol domain‐specific probe reveals the dynamics of the membrane domains during virus release and in Niemann‐Pick type C Open
We identified a novel, nontoxic mushroom protein that specifically binds to a complex of sphingomyelin (SM), a major sphingolipid in mammalian cells, and cholesterol (Chol). The purified protein, termed nakanori, labeled cell surface domai…
View article: Phospholipase Cβ1 induces membrane tubulation and is involved in caveolae formation
Phospholipase Cβ1 induces membrane tubulation and is involved in caveolae formation Open
Significance Lipid membrane curvature plays important roles in various physiological phenomena. Using darkfield microscopy, we performed nonbiased screening of a protein that induces deformations of nonlabeled liposomes. We identified phos…
View article: Deficiency in the Lipid Exporter ABCA1 Impairs Retrograde Sterol Movement and Disrupts Sterol Sensing at the Endoplasmic Reticulum
Deficiency in the Lipid Exporter ABCA1 Impairs Retrograde Sterol Movement and Disrupts Sterol Sensing at the Endoplasmic Reticulum Open
Cellular cholesterol homeostasis involves sterol sensing at the endoplasmic reticulum (ER) and sterol export from the plasma membrane (PM). Sterol sensing at the ER requires efficient sterol delivery from the PM; however, the macromolecule…
View article: Transport through recycling endosomes requires <scp>EHD</scp> 1 recruitment by a phosphatidylserine translocase
Transport through recycling endosomes requires <span>EHD</span> 1 recruitment by a phosphatidylserine translocase Open