Tatsuji Sakamoto
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View article: Genome editing-based mutagenesis stably modifies composition of wax esters synthesized by Euglena gracilis under anaerobic conditions
Genome editing-based mutagenesis stably modifies composition of wax esters synthesized by Euglena gracilis under anaerobic conditions Open
Microalgal oil production represents a promising renewable biofuel source. Metabolic engineering can enhance its utility, transforming it into an improved biofuel and expanding its applications as a feedstock for commodity chemicals, there…
View article: Prunin Laurate Derived from Natural Substances Shows Antibacterial Activity against the Periodontal Pathogen Porphyromonas gingivalis
Prunin Laurate Derived from Natural Substances Shows Antibacterial Activity against the Periodontal Pathogen Porphyromonas gingivalis Open
Periodontal disease is an inflammatory disease caused by infection with periodontopathogenic bacteria. Oral care is essential to prevent and control periodontal disease, which affects oral and systemic health. However, many oral hygiene pr…
View article: Charge neutralization and β-elimination cleavage mechanism of family 42 L-rhamnose-α-1,4-D-glucuronate lyase revealed using neutron crystallography
Charge neutralization and β-elimination cleavage mechanism of family 42 L-rhamnose-α-1,4-D-glucuronate lyase revealed using neutron crystallography Open
View article: Aronia juice improves working memory and suppresses δ-secretase activity in 5XFAD mice
Aronia juice improves working memory and suppresses δ-secretase activity in 5XFAD mice Open
Asparagine endopeptidase (AEP) cleaves amyloid precursor protein to facilitate amyloid β (Aβ) production in the brain. Aronia berry (Aronia melanocarpa) has various beneficial effects on health through inhibition of enzymes such as dipepti…
View article: 【日本応用糖質科学会創立70周年記念特集】2．日本応用糖質科学会70周年記念特別シンポジウム 「澱粉・糖質研究60年で学んだことと将来への期待」
【日本応用糖質科学会創立70周年記念特集】2．日本応用糖質科学会70周年記念特別シンポジウム 「澱粉・糖質研究60年で学んだことと将来への期待」 Open
地球上で最も豊富に存在するバイオマスである植物細胞壁は，脱炭素社会の構築に向けて重要な資源であることは言うまでもない．細胞壁多糖の一成分であるペクチンは，主に双子葉または非イネ科単子葉植物の一次細胞壁および中葉に局在し，ゲル化剤やタンパク質分散安定剤などとして利用されているものの，食品残渣中には多量に残存している．ペクチンは自然界で最も複雑な構造を有する多糖の一つであり，植物種などによりバリエーションはあるが，一般的には7種の異なる糖鎖領域が共有結合して巨大分子を形成してい…
View article: A single mutation Asp43Arg was increased 2.5-fold the catalytic activity and maintained the stability of cold-adapted endo-1,4-beta glucanase (Ef-EG2) from Eisenia fetida
A single mutation Asp43Arg was increased 2.5-fold the catalytic activity and maintained the stability of cold-adapted endo-1,4-beta glucanase (Ef-EG2) from Eisenia fetida Open
To improve the specific activity at low temperatures of Ef-EG2 and to maintain thermostability, five mutant enzymes (K273R, N372D, Q387E, N402D, D43R) were produced. The two mutant enzymes (K273R, N402D) lost the cellulase activity. The sp…
View article: A Single Mutation Glu43arg Was Increased 2.5-Fold the Catalytic Activity and Maintained the Stability of Cold-Adapted Endo-1,4-Beta Glucanase (Ef-Eg2) From Eisenia Fetida
A Single Mutation Glu43arg Was Increased 2.5-Fold the Catalytic Activity and Maintained the Stability of Cold-Adapted Endo-1,4-Beta Glucanase (Ef-Eg2) From Eisenia Fetida Open
View article: Novel acid trehalase belonging to glycoside hydrolase family 37 from <i>Pleurotus</i> sp.: cloning, expression and characterization
Novel acid trehalase belonging to glycoside hydrolase family 37 from <i>Pleurotus</i> sp.: cloning, expression and characterization Open
The N-terminal amino acid sequence of the Pleurotus sp. 90 kDa protein was in good agreement with the corresponding sequence of the glycoside hydrolase (GH) family 37 protein (trehalase) from P. ostreatus PC 15 v2.0. The leng…
View article: Charge neutralization and β-elimination cleavage mechanism of family 42 L-rhamnose-α-1,4-D-glucuronate lyase revealed using neutron crystallography
Charge neutralization and β-elimination cleavage mechanism of family 42 L-rhamnose-α-1,4-D-glucuronate lyase revealed using neutron crystallography Open
Gum arabic (GA) is widely used as an emulsion stabilizer and edible coating, and consists of a complex carbohydrate moiety with a rhamnosyl-glucuronate group capping the non-reducing ends. Enzymes that can specifically cleave the glycosidi…
View article: [Review] Structure-function Analysis of <i>Fusarium oxysporum</i> Enzymes that Degrade Terminals of Gum Arabic Side-chains
[Review] Structure-function Analysis of <i>Fusarium oxysporum</i> Enzymes that Degrade Terminals of Gum Arabic Side-chains Open
我々は,アラビアガムの糖鎖側鎖末端に位置するキャップ残基α-L-Rha-1,4-β-D-GlcA (Rha-GlcA) を分解する2種の新規酵素 (FoBGlcAおよびFoRham1) をFusarium oxysporum 12S株から単離した.FoBGlcAはRha-GlcAを遊離する新規な基質認識を持ち,糖質加水分解酵素ファミリー79 (GH79) に分類される4-O-α-L-ラムノシル-β-D-グルクロニダーゼであることが明らかになった.また構造解析の結果,サブサイ…
View article: NADPH‐to‐NADH conversion by mitochondrial transhydrogenase is indispensable for sustaining anaerobic metabolism in <i>Euglena gracilis</i>
NADPH‐to‐NADH conversion by mitochondrial transhydrogenase is indispensable for sustaining anaerobic metabolism in <i>Euglena gracilis</i> Open
Euglena gracilis produces ATP in the anaerobic mitochondria with concomitant wax ester formation, and NADH is essential for ATP formation and fatty acid synthesis in the mitochondria. This study demonstrated that mitochondrial cofactor con…
View article: Exopolysaccharides from a Scandinavian fermented milk viili increase butyric acid and Muribaculum members in the mouse gut
Exopolysaccharides from a Scandinavian fermented milk viili increase butyric acid and Muribaculum members in the mouse gut Open
Starter culture of viili contains lactic acid bacteria belonging to Lactococcus lactis. These bacteria secrete large polysaccharides (EPSs) into milk, resulting in a ropy texture of viili. In mouse experiments, a large dose of EPS (…
View article: Novel cold-adapted raw-starch digesting α-amylases from Eisenia fetida: Gene cloning, expression, and characterization
Novel cold-adapted raw-starch digesting α-amylases from Eisenia fetida: Gene cloning, expression, and characterization Open
View article: Homogalacturonan and xylogalacturonan region specificity of self-cloning vector-expressed pectin methylesterases (AoPME1–3) in Aspergillus oryzae
Homogalacturonan and xylogalacturonan region specificity of self-cloning vector-expressed pectin methylesterases (AoPME1–3) in Aspergillus oryzae Open
View article: Structural and functional analysis of gum arabic l-rhamnose-α-1,4-d-glucuronate lyase establishes a novel polysaccharide lyase family
Structural and functional analysis of gum arabic l-rhamnose-α-1,4-d-glucuronate lyase establishes a novel polysaccharide lyase family Open
Gum arabic (GA) is widely used as an emulsion stabilizer and coating in several industrial applications, such as foods and pharmaceuticals. GA contains a complex carbohydrate moiety, and the nonreducing ends of the side chains are often ca…
View article: Biochemical and structural characterization of a novel 4‐ <i>O</i> ‐α‐ <scp>l</scp> ‐rhamnosyl‐β‐ <scp>d</scp> ‐glucuronidase from <i>Fusarium oxysporum</i>
Biochemical and structural characterization of a novel 4‐ <i>O</i> ‐α‐ <span>l</span> ‐rhamnosyl‐β‐ <span>d</span> ‐glucuronidase from <i>Fusarium oxysporum</i> Open
In this study, we have isolated the novel enzyme 4‐ O ‐α‐ l ‐rhamnosyl‐β‐ d ‐glucuronidase (FoBGlcA), which releases α‐ l ‐rhamnosyl (1→4) glucuronic acid from gum arabic (GA), from Fusarium oxysporum 12S culture supernatant, and for the f…
View article: Author response for "Biochemical and structural characterization of a novel 4-O-alpha-l-rhamnosyl-beta-d-glucuronidase from Fusarium oxysporum"
Author response for "Biochemical and structural characterization of a novel 4-O-alpha-l-rhamnosyl-beta-d-glucuronidase from Fusarium oxysporum" Open
View article: Reduction of blood glucose and HbA1c levels by cyanidin 3,5-diglucoside in KKAy mice
Reduction of blood glucose and HbA1c levels by cyanidin 3,5-diglucoside in KKAy mice Open
View article: Anaerobic respiration coupled with mitochondrial fatty acid synthesis in wax ester fermentation by <i>Euglena gracilis</i>
Anaerobic respiration coupled with mitochondrial fatty acid synthesis in wax ester fermentation by <i>Euglena gracilis</i> Open
In Euglena gracilis , wax ester fermentation produces ATP during anaerobiosis. Here, we report that anaerobic wax ester production is suppressed when the mitochondrial electron transport chain complex I is inhibited by rotenone, whereas it…
View article: Gene cloning, expression, and X-ray crystallographic analysis of a β-mannanase from Eisenia fetida
Gene cloning, expression, and X-ray crystallographic analysis of a β-mannanase from Eisenia fetida Open
View article: Lactic Acid Bacteria from Kefir Increase Cytotoxicity of Natural Killer Cells to Tumor Cells
Lactic Acid Bacteria from Kefir Increase Cytotoxicity of Natural Killer Cells to Tumor Cells Open
The Japanese fermented beverage, homemade kefir, contains six lactic acid bacteria: Lactococcus. lactis subsp. Lactis, Lactococcus. lactis subsp. Cremoris, Lactococcus. Lactis subsp. Lactis biovar diacetylactis, Lactobacillus plantarum, Le…
View article: Crystal structure of exo‐rhamnogalacturonan lyase from <i>Penicillium chrysogenum</i> as a member of polysaccharide lyase family 26
Crystal structure of exo‐rhamnogalacturonan lyase from <i>Penicillium chrysogenum</i> as a member of polysaccharide lyase family 26 Open
Exo‐rhamnogalacturonan lyase from Penicillium chrysogenum 31B (Pc RGLX ) was recently classified as a member of polysaccharide lyase ( PL ) family 26 along with hypothetical proteins derived from various organisms. In this study, we determ…
View article: Secretion of Legumain Increases in Conditioned Medium from DJ-1-Knockout Cells and in Serum from DJ-1-Knockout Mice
Secretion of Legumain Increases in Conditioned Medium from DJ-1-Knockout Cells and in Serum from DJ-1-Knockout Mice Open
Asparaginyl endopeptidase, also known as legumain (EC 3.4.22.34) shows strong activity in the mouse kidney. Legumain is also highly expressed in tumors. DJ-1/PARK7 is a Parkinson's disease- and cancer-associated protein. DJ-1 is a coactiva…
View article: Ferulic acid and its water-soluble derivatives inhibit nitric oxide production and inducible nitric oxide synthase expression in rat primary astrocytes
Ferulic acid and its water-soluble derivatives inhibit nitric oxide production and inducible nitric oxide synthase expression in rat primary astrocytes Open
We recently reported that two water-soluble derivatives of ferulic acid (1-feruloyl glycerol, 1-feruloyl diglycerol) previously developed by our group exhibited protective effects against amyloid-β–induced neurodegeneration in vitro and in…
View article: Physiological functions of pyruvate:NADP+ oxidoreductase and 2-oxoglutarate decarboxylase in <i>Euglena gracilis</i> under aerobic and anaerobic conditions
Physiological functions of pyruvate:NADP+ oxidoreductase and 2-oxoglutarate decarboxylase in <i>Euglena gracilis</i> under aerobic and anaerobic conditions Open
In Euglena gracilis, pyruvate:NADP+ oxidoreductase, in addition to the pyruvate dehydrogenase complex, functions for the oxidative decarboxylation of pyruvate in the mitochondria. Furthermore, the 2-oxoglutarate dehydrogenase complex is ab…
View article: Reduction of blood pressure by aronia berries through inhibition of angiotensin-converting enzyme activity in the spontaneously hypertensive rat kidney
Reduction of blood pressure by aronia berries through inhibition of angiotensin-converting enzyme activity in the spontaneously hypertensive rat kidney Open
Background: Aronia berries contain many important effects on potential health, with previous studies suggesting that aronia juice is useful for the treatment of hypertension.Objective: To examine whether aronia berries have improvement eff…
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Correction Open
View article: Aronia juice suppresses the elevation of postprandial blood glucose levels in adult healthy Japanese
Aronia juice suppresses the elevation of postprandial blood glucose levels in adult healthy Japanese Open
Aronia has various functions toward human health, including the beneficial effect on hypertension, hyperglycemia and hyperlipidemia. Recently, we identified cyanidin-3,5-O-diglucoside as DPP IV inhibitor from Aronia juice. We also found it…
View article: Water-soluble ferulic acid derivatives improve amyloid-β-induced neuronal cell death and dysmnesia through inhibition of amyloid-β aggregation
Water-soluble ferulic acid derivatives improve amyloid-β-induced neuronal cell death and dysmnesia through inhibition of amyloid-β aggregation Open
Ferulic acid (FA) has been reported to exhibit protective effects against amyloid-β (Aβ)-induced neurodegeneration in vitro and in vivo. Recently, we developed two water-soluble FA derivatives: 1-feruloyl glycerol and 1-feruloyl diglycerol…