John T. Groves
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View article: Engineering auxin degradation into root-associated bacteria promotes plant growth
Engineering auxin degradation into root-associated bacteria promotes plant growth Open
Overproduction of indole-3-acetic acid (IAA) by rhizosphere bacteria disrupts auxin homeostasis and induces root growth inhibition (RGI) in plants. Bacteria from the genus Variovorax mitigate this effect by degrading IAA, and in our prior …
View article: Unspecific Peroxygenase Catalyzes Selective Remote‐Site Functionalizations
Unspecific Peroxygenase Catalyzes Selective Remote‐Site Functionalizations Open
We describe the discovery of an unspecific peroxygenase (UPO) variant that catalyzes the remote‐site functionalization of halogenated and unsaturated hydrocarbons with high catalytic site‐specificity. UPOs are fungal heme‐thiolate biocatal…
View article: Two bovine hepacivirus genome sequences from U.S. cattle
Two bovine hepacivirus genome sequences from U.S. cattle Open
Bovine hepacivirus (BoHV) is closely related to the hepatitis C virus (HCV) in humans and can cause both acute and chronic liver infections in cattle. BoHV was first identified in Ghana and Germany in 2015 and since then it has been detect…
View article: Compositions and methods for hydrocarbon functionalization
Compositions and methods for hydrocarbon functionalization Open
Embodiments of the present disclosure provide for methods of hydrocarbon functionalization, methods and systems for converting a hydrocarbon into a compound including at least one group ((e.g., hydroxyl group) (e.g., methane to methanol)),…
View article: Structure and Function of Alkane Monooxygenase (AlkB)
Structure and Function of Alkane Monooxygenase (AlkB) Open
Every year, perhaps as much as 800 million tons of hydrocarbons enters the environment; alkanes make up a large percentage of it. Most are transformed by organisms that utilize these molecules as sources of energy and carbon. Both aerobic …
View article: Spatial biomarker of disease and detection of spatial organization of cellular receptors
Spatial biomarker of disease and detection of spatial organization of cellular receptors Open
A signature of a condition of a live cell is established in an assay that allows distribution of the receptors on the cell surface in response to binding a ligand. The receptors can be optically detected and quantified to provide a value f…
View article: Detection of molecular interactions
Detection of molecular interactions Open
A method and assay are described for measuring the interaction between a ligand and an analyte. The assay can include a suspension of colloidal particles that are associated with a ligand of interest. The colloidal particles are maintained…
View article: C-halogen bond formation
C-halogen bond formation Open
Methods of halogenating a carbon containing compound having an sp3 C--H bond are provided. Methods of fluorinating a carbon containing compound comprising halogenation with Cl or Br followed by nucleophilic substitution with F are provided…
View article: Assessing the Role of Systems Thinking for Stocker Cattle Operations
Assessing the Role of Systems Thinking for Stocker Cattle Operations Open
Bovine respiratory disease (BRD) is recognized as a complex multifactorial disease often resulting in significant economic losses for the stocker industry through reduced health and performance of feeder calves. Conventional approaches to …
View article: Clinical and microbiological effects in high-risk beef calves administered intranasal or parenteral modified-live virus vaccines
Clinical and microbiological effects in high-risk beef calves administered intranasal or parenteral modified-live virus vaccines Open
Experimental bovine respiratory syncytial virus (BRSV) infection can enhance Histophilus somni (Hs) disease in calves; we thus hypothesized that modified-live virus (MLV) vaccines containing BRSV may alter Hs carriage. Our objective was to…
View article: CCDC 2131612: Experimental Crystal Structure Determination
CCDC 2131612: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: Manganese Catalyzed Partial Oxidation of Light Alkanes
Manganese Catalyzed Partial Oxidation of Light Alkanes Open
The catalytic partial oxidation of methane is achieved at low temperatures (90% at ∼35% methane conversion at approximately 6 turnovers. Under our catalytic methane oxidation reaction conditions, MeTFA is stable against overoxidation, whic…
View article: Aerobic Partial Oxidation of Alkanes Using Photodriven Iron Catalysis
Aerobic Partial Oxidation of Alkanes Using Photodriven Iron Catalysis Open
Photodriven oxidations of alkanes in trifluoroacetic acid using commercial and synthesized Fe(III) sources as catalyst precursors and dioxygen (O2) as the terminal oxidant are reported. The reactions produce alkyl esters and occur at ambie…
View article: Selective Photo‐Oxygenation of Light Alkanes Using Iodine Oxides and Chloride
Selective Photo‐Oxygenation of Light Alkanes Using Iodine Oxides and Chloride Open
Partial oxidation of light alkanes to generate alkyl esters has been achieved under photochemical conditions using mixtures of iodine oxides and chloride salts in trifluoroacetic acid (HTFA). The reactions are catalytic in chloride and are…
View article: Photoinduced charge flow inside an iron porphyrazine complex
Photoinduced charge flow inside an iron porphyrazine complex Open
Sub-picosecond electronic and structural motions and MLCT/d–d intersystem crossing in an iron-porphyrazine are revealed using transient absorption and femtosecond stimulated-Raman spectroscopy.
View article: Becoming indispensable by using "Systems Thinking" to tackle challenging and complex problems in practice
Becoming indispensable by using "Systems Thinking" to tackle challenging and complex problems in practice Open
Many veterinary practitioners and consultants play critical and indispensable roles in the management and decision-making processes of large, complex production structures. The discipline of Systems Thinking offers an opportunity and metho…
View article: Mechanism of Hydrocarbon Functionalization by an Iodate/Chloride System: The Role of Ester Protection
Mechanism of Hydrocarbon Functionalization by an Iodate/Chloride System: The Role of Ester Protection Open
Mixtures of chloride and iodate salts for light alkane oxidation achieve > 20% yield of methyl trifluoroacetate (TFA) from methane with > 85% selectivity (J. Am. Chem. Soc. 2014, 136, 8393-8401). The mechanism of this C–H oxygenation has b…
View article: Immune-modulating enzyme indoleamine 2,3-dioxygenase is effectively inhibited by targeting its apo-form
Immune-modulating enzyme indoleamine 2,3-dioxygenase is effectively inhibited by targeting its apo-form Open
Significance Indoleamine 2,3-dioxygenase (IDO1) is a heme protein that catalyzes the dioxygenation of tryptophan. Cells expressing this activity are able to profoundly alter their surrounding environment to suppress the immune response. Ca…
View article: Oxygen Activation and Radical Transformations in Heme Proteins and Metalloporphyrins
Oxygen Activation and Radical Transformations in Heme Proteins and Metalloporphyrins Open
As a result of the adaptation of life to an aerobic environment, nature has evolved a panoply of metalloproteins for oxidative metabolism and protection against reactive oxygen species. Despite the diverse structures and functions of these…
View article: Site-selective <sup>18</sup>F fluorination of unactivated C–H bonds mediated by a manganese porphyrin
Site-selective <sup>18</sup>F fluorination of unactivated C–H bonds mediated by a manganese porphyrin Open
A direct aliphatic C–H 18F labeling method using [18F]fluoride ion at inaccessible and unreactive sites is reported.
View article: The Enigmatic P450 Decarboxylase OleT Is Capable of, but Evolved To Frustrate, Oxygen Rebound Chemistry
The Enigmatic P450 Decarboxylase OleT Is Capable of, but Evolved To Frustrate, Oxygen Rebound Chemistry Open
OleT is a cytochrome P450 enzyme that catalyzes the removal of carbon dioxide from variable chain length fatty acids to form 1-alkenes. In this work, we examine the binding and metabolic profile of OleT with shorter chain length (n ≤ 12) f…
View article: Fast Hydrogen Atom Abstraction by a Hydroxo Iron(III) Porphyrazine
Fast Hydrogen Atom Abstraction by a Hydroxo Iron(III) Porphyrazine Open
A reactive hydroxoferric porphyrazine complex, [(PyPz)FeIII(OH) (OH2)]4+ (1, PyPz = tetramethyl-2,3-pyridino porphyrazine), has been prepared via one-electron oxidation of the corresponding ferrous species [(PyPz)FeII(OH2)2]4+ (2). Electro…
View article: Cytochrome P450 enzymes: understanding the biochemical hieroglyphs
Cytochrome P450 enzymes: understanding the biochemical hieroglyphs Open
Cytochrome P450 (CYP) enzymes are the primary proteins of drug metabolism and steroid biosynthesis. These crucial proteins have long been known to harbor a cysteine thiolate bound to the heme iron. Recent advances in the field have illumin…
View article: Heme-thiolate ferryl of aromatic peroxygenase is basic and reactive
Heme-thiolate ferryl of aromatic peroxygenase is basic and reactive Open
Significance The heme-thiolate peroxygenase of Agrocybe aegerita is a remarkably capable biocatalyst and a mechanistic analog of cytochrome P450. The stability of this fungal protein has provided a rare opportunity to study P450-like C−H h…
View article: Ferryl Protonation in Oxoiron(IV) Porphyrins and Its Role in Oxygen Transfer
Ferryl Protonation in Oxoiron(IV) Porphyrins and Its Role in Oxygen Transfer Open
Ferryl porphyrins, P-Fe(IV)═O, are central reactive intermediates in the catalytic cycles of numerous heme proteins and a variety of model systems. There has been considerable interest in elucidating factors, such as terminal oxo basicity,…