Ted Hackstadt
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View article: <i>Rickettsia rickettsii</i> RoaM negatively regulates expression of a limited number of rickettsial genes
<i>Rickettsia rickettsii</i> RoaM negatively regulates expression of a limited number of rickettsial genes Open
The recently described rickettsial protein RoaM (regulator of actin-based motility) negatively regulates the production of actin tails, and its abrogation induces hyper-spreading behavior in many laboratory-adapted strains of Rickettsia ri…
View article: Development of inducible promoter and CRISPRi plasmids functional in <i>Rickettsia rickettsii</i>
Development of inducible promoter and CRISPRi plasmids functional in <i>Rickettsia rickettsii</i> Open
Rickettsia rickettsii is an obligate intracellular, tick-borne bacterium that causes Rocky Mountain spotted fever. The demanding nature of cultivating these bacteria within host cells and the labor involved in obtaining clonal isolates hav…
View article: Development of inducible promoter and CRISPRi plasmids functional in<i>Rickettsia rickettsii</i>
Development of inducible promoter and CRISPRi plasmids functional in<i>Rickettsia rickettsii</i> Open
Rickettsia rickettsii is an obligate intracellular, tick-borne bacterium that causes Rocky Mountain spotted fever. The demanding nature of cultivating these bacteria within host cells and the labor involved in obtaining clonal isolates has…
View article: <i>Rickettsia rickettsii</i> virulence determinants RARP2 and RapL mitigate IFN- <b>β</b> signaling in primary human dermal microvascular endothelial cells
<i>Rickettsia rickettsii</i> virulence determinants RARP2 and RapL mitigate IFN- <b>β</b> signaling in primary human dermal microvascular endothelial cells Open
We compared the growth characteristics of a virulent Rickettsia rickettsii strain (Sheila Smith) to an attenuated R. rickettsii stain (Iowa) and a non-pathogenic species ( R. montanensis ) in primary human dermal microvascular endothelial …
View article: Completed genomes for <i>Rickettsia rickettsii</i> isolated from ticks and quality controlled for motility phenotype
Completed genomes for <i>Rickettsia rickettsii</i> isolated from ticks and quality controlled for motility phenotype Open
Complete genomes of Rickettsia rickettsii were sequenced with Illumina and PacBio technologies from low-passage isolates from ticks. These isolates were quality controlled for intact roaM , a regulator of actin-based motility that is negat…
View article: Identification of an autotransporter peptidase of Rickettsia rickettsii responsible for maturation of surface exposed autotransporters
Identification of an autotransporter peptidase of Rickettsia rickettsii responsible for maturation of surface exposed autotransporters Open
Members of the spotted fever group rickettsia express four large, surface-exposed autotransporters, at least one of which is a known virulence determinant. Autotransporter translocation to the bacterial outer surface, also known as type V …
View article: Chlamydia trachomatis Alters Mitochondrial Protein Composition and Secretes Effector Proteins That Target Mitochondria
Chlamydia trachomatis Alters Mitochondrial Protein Composition and Secretes Effector Proteins That Target Mitochondria Open
Obligate intracellular bacteria have evolved multiple means to promote their intracellular survival and replication within the otherwise harsh environment of the eukaryotic cell. Nutrient acquisition and avoidance of cellular defense mecha…
View article: <i>Chlamydia trachomatis</i> suppresses host cell store-operated Ca<sup>2+</sup> entry and inhibits NFAT/calcineurin signaling
<i>Chlamydia trachomatis</i> suppresses host cell store-operated Ca<sup>2+</sup> entry and inhibits NFAT/calcineurin signaling Open
The obligate intracellular bacterium, Chlamydia trachomatis , replicates within a parasitophorous vacuole termed an inclusion. During development, host proteins critical for regulating intracellular calcium (Ca 2+ ) homeostasis interact wi…
View article: Regulator of Actin-Based Motility (RoaM) Downregulates Actin Tail Formation by Rickettsia rickettsii and Is Negatively Selected in Mammalian Cell Culture
Regulator of Actin-Based Motility (RoaM) Downregulates Actin Tail Formation by Rickettsia rickettsii and Is Negatively Selected in Mammalian Cell Culture Open
The mechanism of actin-based motility of spotted fever group Rickettsia has been studied extensively, but here, we provide genetic evidence that motility is a regulated process in R. rickettsii . The findings also suggest that serial passa…
View article: Disruption of the Golgi Apparatus and Contribution of the Endoplasmic Reticulum to the SARS-CoV-2 Replication Complex
Disruption of the Golgi Apparatus and Contribution of the Endoplasmic Reticulum to the SARS-CoV-2 Replication Complex Open
A variety of immunolabeling procedures for both light and electron microscopy were used to examine the cellular origins of the host membranes supporting the SARS-CoV-2 replication complex. The endoplasmic reticulum has long been implicated…
View article: Selective fragmentation of the trans-Golgi apparatus by Rickettsia rickettsii
Selective fragmentation of the trans-Golgi apparatus by Rickettsia rickettsii Open
Fragmentation of the Golgi apparatus is observed during a number of physiological processes including mitosis and apoptosis, but also occurs in pathological states such as neurodegenerative diseases and some infectious diseases. Here we sh…
View article: Chlamydia trachomatis CT229 Subverts Rab GTPase-Dependent CCV Trafficking Pathways to Promote Chlamydial Infection
Chlamydia trachomatis CT229 Subverts Rab GTPase-Dependent CCV Trafficking Pathways to Promote Chlamydial Infection Open
Chlamydial infection requires the formation of a membrane-bound vacuole, termed the inclusion, that undergoes extensive interactions with select host organelles. The importance of the Inc protein CT229 in the formation and maintenance of t…
View article: The Rickettsial Ankyrin Repeat Protein 2 Is a Type IV Secreted Effector That Associates with the Endoplasmic Reticulum
The Rickettsial Ankyrin Repeat Protein 2 Is a Type IV Secreted Effector That Associates with the Endoplasmic Reticulum Open
Strains of Rickettsia rickettsii , the tick-borne agent of Rocky Mountain spotted fever, vary considerably in virulence. Genomic comparisons of R. rickettsii strains have identified a relatively small number of genes divergent in an avirul…
View article: Impact of Active Metabolism on Chlamydia trachomatis Elementary Body Transcript Profile and Infectivity
Impact of Active Metabolism on Chlamydia trachomatis Elementary Body Transcript Profile and Infectivity Open
Bacteria of the genus Chlamydia include the significant human pathogens Chlamydia trachomatis and C. pneumoniae . All chlamydiae are obligate intracellular parasites that depend on infection of a host cell and transition through a biphasic…
View article: Chlamydia trachomatis inclusion membrane protein MrcA interacts with the inositol 1,4,5-trisphosphate receptor type 3 (ITPR3) to regulate extrusion formation
Chlamydia trachomatis inclusion membrane protein MrcA interacts with the inositol 1,4,5-trisphosphate receptor type 3 (ITPR3) to regulate extrusion formation Open
Chlamydia trachomatis is an obligate intracellular bacterium that replicates within a vacuole termed an inclusion. At the end of their intracellular developmental cycle, chlamydiae are released either by lysis of the host cell or extrusion…
View article: <i>Chlamydia</i> Hijacks ARF GTPases To Coordinate Microtubule Posttranslational Modifications and Golgi Complex Positioning
<i>Chlamydia</i> Hijacks ARF GTPases To Coordinate Microtubule Posttranslational Modifications and Golgi Complex Positioning Open
The intracellular bacterium Chlamydia trachomatis develops in a parasitic compartment called the inclusion. Posttranslationally modified microtubules encase the inclusion, controlling the positioning of Golgi complex fragments around the i…
View article: Absence of Specific Chlamydia trachomatis Inclusion Membrane Proteins Triggers Premature Inclusion Membrane Lysis and Host Cell Death
Absence of Specific Chlamydia trachomatis Inclusion Membrane Proteins Triggers Premature Inclusion Membrane Lysis and Host Cell Death Open
Chlamydia trachomatis is a human pathogen associated with significant morbidity worldwide. As obligate intracellular parasites, chlamydiae must survive within eukaryotic cells for sufficient time to complete their developmental cycle. To p…
View article: Proteolytic Cleavage of the Immunodominant Outer Membrane Protein rOmpA in Rickettsia rickettsii
Proteolytic Cleavage of the Immunodominant Outer Membrane Protein rOmpA in Rickettsia rickettsii Open
Rickettsia rickettsii , the causative agent of Rocky Mountain spotted fever, contains two immunodominant proteins, rOmpA and rOmpB, in the outer membrane. Both rOmpA and rOmpB are conserved throughout spotted fever group rickettsiae as mem…
View article: A Functional Core of IncA Is Required for Chlamydia trachomatis Inclusion Fusion
A Functional Core of IncA Is Required for Chlamydia trachomatis Inclusion Fusion Open
Chlamydia trachomatis is an obligate intracellular pathogen that is the etiological agent of a variety of human diseases, including blinding trachoma and sexually transmitted infections. Chlamydiae replicate within a membrane-bound compart…
View article: Expression and Localization of Predicted Inclusion Membrane Proteins in Chlamydia trachomatis
Expression and Localization of Predicted Inclusion Membrane Proteins in Chlamydia trachomatis Open
Chlamydia trachomatis is an obligate intracellular pathogen that replicates in a membrane-bound vacuole termed the inclusion. Early in the infection cycle, the pathogen extensively modifies the inclusion membrane through incorporation of n…
View article: Targeted Knockout of the Rickettsia rickettsii OmpA Surface Antigen Does Not Diminish Virulence in a Mammalian Model System
Targeted Knockout of the Rickettsia rickettsii OmpA Surface Antigen Does Not Diminish Virulence in a Mammalian Model System Open
Strains of Rickettsia rickettsii , the causative agent of Rocky Mountain spotted fever (RMSF), differ dramatically in virulence despite >99% genetic homology. Spotted fever group (SFG) rickettsiae produce two immunodominant outer membrane …
View article: Comparative Genome Sequencing of Rickettsia rickettsii Strains That Differ in Virulence
Comparative Genome Sequencing of Rickettsia rickettsii Strains That Differ in Virulence Open
Rickettsia rickettsii is an obligate intracellular pathogen that is the causative agent of Rocky Mountain spotted fever. Strains of R. rickettsii differ dramatically in virulence. In a guinea pig model of infection, the severity of disease…