Tegan A. Otto
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View article: Spatial control of karyopherin binding avidity within NPC mimics revealed by designer FG- Nucleoporins
Spatial control of karyopherin binding avidity within NPC mimics revealed by designer FG- Nucleoporins Open
Nucleocytoplasmic transport occurs via nuclear pore complexes (NPCs), ∼40-60 nm wide pores lined with intrinsically disordered proteins that are rich in Phe-Gly motifs (FG-Nups) that form a selective barrier. Molecules larger than ∼50 kDa …
View article: Imaging-based quantitative assessment of biomolecular condensates in vitro and in cells
Imaging-based quantitative assessment of biomolecular condensates in vitro and in cells Open
The formation of biomolecular condensates contributes to intracellular compartmentalization and plays an important role in many cellular processes. The characterization of condensates is however challenging, requiring advanced biophysical …
View article: PunctaFinder: An algorithm for automated spot detection in fluorescence microscopy images
PunctaFinder: An algorithm for automated spot detection in fluorescence microscopy images Open
Fluorescence microscopy has revolutionized biological research by enabling the visualization of subcellular structures at high resolution. With the increasing complexity and volume of microscopy data, there is a growing need for automated …
View article: A survey of the specificity and mechanism of 1,6 hexanediol-induced disruption of nuclear transport
A survey of the specificity and mechanism of 1,6 hexanediol-induced disruption of nuclear transport Open
Selective transport through the nuclear pore complex (NPC) depends on the dynamic binding of FG-repeat containing nucleoporins, the FG-nups, with each other and with Karyopherins (Kaps). Here, we assessed the specificity and mechanism by w…
View article: Nucleoporin Nsp1 surveils the phase state of FG-Nups
Nucleoporin Nsp1 surveils the phase state of FG-Nups Open
Transport through the NPC relies on intrinsically disordered FG-Nups forming a selective barrier. Away from the NPC, FG-Nups readily form condensates and aggregates, and we address how this behavior is surveilled in cells. FG-Nups, includi…
View article: Specificity and mechanism of 1,6 hexanediol-induced disruption of nuclear transport
Specificity and mechanism of 1,6 hexanediol-induced disruption of nuclear transport Open
Selective transport through the nuclear pore complex (NPC) depends on the dynamic binding of the intrinsically disordered components of the NPC, the FG-nups, with each other and with nuclear transport receptors (NTRs). Hydrophobic interact…
View article: Additional file 1 of VISTA regulates microglia homeostasis and myelin phagocytosis, and is associated with MS lesion pathology
Additional file 1 of VISTA regulates microglia homeostasis and myelin phagocytosis, and is associated with MS lesion pathology Open
Additional file 1. Extended data containing Tables S1-S5. Table S1. Information on MS patients. Table S2. Morphometric features of individual cells. Table S3. Genes in published microglia gene sets. Table S4. Normcounts and differential ge…