Daniel R. Tershak
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View article: Guanidine-resistant mutants of poliovirus have distinct mutations in peptide 2C
Guanidine-resistant mutants of poliovirus have distinct mutations in peptide 2C Open
In previous work in our laboratory, 12 guanidine-resistant (gr) mutants of poliovirus were selected from 12 separate stocks of plaque-purified guanidine-sensitive (gs) viruses (K. Anderson-Sillman, S. Bartal, and D. R. Tershak, J. Virol. 5…
View article: Association of poliovirus proteins with the endoplasmic reticulum
Association of poliovirus proteins with the endoplasmic reticulum Open
Poliovirus proteins, except P3-7c, are associated with the endoplasmic reticulum after extraction of the cytoplasm and centrifugation of membranes to equilibrium in sucrose gradients. Proteins P3-2, P2-X, and P3-9 are found preferentially …
View article: Guanidine-resistant poliovirus mutants produce modified 37-kilodalton proteins
Guanidine-resistant poliovirus mutants produce modified 37-kilodalton proteins Open
Eighteen spontaneous, guanidine-resistant mutants of poliovirus were obtained by plaque selection. Isoelectric focusing demonstrated charge changes in a 37-kilodalton protein, pX, among three of the mutants. The precursor of pX, NCVP5b , a…
View article: Guanidine-resistant defective interfering particles of poliovirus
Guanidine-resistant defective interfering particles of poliovirus Open
A mixture containing standard poliovirus and D3 particles (mutants with deletions in the capsid locus) was serially passaged in the presence of guanidine. Within five growth cycles, the standard virus was guanidine resistant, but the D3 pa…
View article: Inhibition of poliovirus polymerase by guanidine in vitro
Inhibition of poliovirus polymerase by guanidine in vitro Open
Extracts from poliovirus-infected HeLa cells synthesized 35s viral RNA, replicative intermediate, and double-stranded RNA in vitro. Guanidine inhibited the synthesis of all three species of RNA; production of 35s RNA was most sensitive to …
View article: Peptide-chain initiation with Lsc poliovirus is intrinsically more resistant to hypertonic environment than is peptide-chain initiation with Mahoney virus and deletion mutants of Mahoney virus
Peptide-chain initiation with Lsc poliovirus is intrinsically more resistant to hypertonic environment than is peptide-chain initiation with Mahoney virus and deletion mutants of Mahoney virus Open
Peptide-chain initiation with LSc poliovirus was more resistant to hypertonic medium than peptide-chain initiation with Mahoney poliovirus. Protein synthesis of LSc virus retained its relative resistance to high osmolarity created by the a…
View article: Proteolysis of noncapsid protein 2 of type 3 poliovirus at the restrictive temperature: breakdown of noncapsid protein 2 correlates with loss of RNA synthesis
Proteolysis of noncapsid protein 2 of type 3 poliovirus at the restrictive temperature: breakdown of noncapsid protein 2 correlates with loss of RNA synthesis Open
Inhibition of RNA synthesis of the LH strain of type 3 poliovirus at the restrictive temperature occurs concurrently with the breakdown of noncapsid virus-specific peptide 2. This finding, along with other reported data (B.D. Korant, In Pr…
View article: Effect of hypertonic medium on protein synthesis of Mahoney and LSc poliovirus
Effect of hypertonic medium on protein synthesis of Mahoney and LSc poliovirus Open
The initiation of synthesis of Mahoney virus protein is more sensitive to high osmolarity than initiation of synthesis of LSc virus protein. Production of Mahoney virus protein appears to be only slightly more resistant to high osmolarity …
View article: Synthesis of Ribonucleic Acid in Cells Infected with LSc Poliovirus at Elevated Temperatures
Synthesis of Ribonucleic Acid in Cells Infected with LSc Poliovirus at Elevated Temperatures Open
The synthesis of viral ribonucleic acid (RNA) was detected within 2 hr after infection with LSc poliovirus at 35 C. This RNA eluted as a single peak with 0.9 m NaCl on methylated albumin celite columns, was sensitive to ribonuclease, preci…
View article: Rescue of Temperature-sensitive Poliovirus
Rescue of Temperature-sensitive Poliovirus Open
A temperature-sensitive strain of type 1 poliovirus, LSc, was functionally rescued when infected cells were incubated at 40 C in the presence of Mahoney, a temperature-resistant strain of type 1 poliovirus. The rescue value was 9% of the m…