Tessa Van de Steene
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View article: Rapid assay optimization by plug-and-play MAPPIT
Rapid assay optimization by plug-and-play MAPPIT Open
Studying protein-protein interactions, which are crucial for all cellular processes in both health and disease, offers valuable insights into underlying mechanisms and reveals promising therapeutic targets. While efforts have been made to …
View article: Virotrap Reveals <i>Salmonella</i> SopB as A Ubiquitinated Cargo for Host ESCRT-0
Virotrap Reveals <i>Salmonella</i> SopB as A Ubiquitinated Cargo for Host ESCRT-0 Open
The pathogenic bacterium Salmonella survives and replicates in host cells within a Salmonella -containing vacuole (SCV). To build this niche, Salmonella uses secreted effectors, such as SopB, a phosphoinositide phosphotransferase that modi…
View article: A 96‐Well Ultrafiltration Approach for the High‐Throughput Proteome Analysis of Extracellular Vesicles Isolated From Conditioned Medium
A 96‐Well Ultrafiltration Approach for the High‐Throughput Proteome Analysis of Extracellular Vesicles Isolated From Conditioned Medium Open
Extracellular vesicles (EVs), nanoscale vesicles that are secreted by cells, are critical mediators of intercellular communication and play a crucial role in diverse pathologies such as cancer development. Therefore, EVs are regarded as ha…
View article: Filter-Aided Extracellular Vesicle Enrichment (FAEVEr) for Proteomics
Filter-Aided Extracellular Vesicle Enrichment (FAEVEr) for Proteomics Open
Extracellular vesicles (EVs), membrane-delimited nanovesicles that are secreted by cells into the extracellular environment, are gaining substantial interest due to their involvement in cellular homeostasis and their contribution to diseas…
View article: Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics
Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics Open
Protein-protein interactions play an important biological role in every aspect of cellular homeostasis and functioning. Proximity labeling mass spectrometry-based proteomics overcomes challenges typically associated with other methods and …
View article: Cornification of keratinocytes is associated with differential changes in the catalytic activity and the immunoreactivity of transglutaminase-1
Cornification of keratinocytes is associated with differential changes in the catalytic activity and the immunoreactivity of transglutaminase-1 Open
View article: Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics
Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics Open
Summary Protein-protein interactions play an important biological role in every aspect of cellular homeostasis and functioning. Proximity labeling mass spectrometry-based proteomics overcomes challenges typically associated with other meth…
View article: Filter-aided extracellular vesicle enrichment (FAEVEr) for proteomics
Filter-aided extracellular vesicle enrichment (FAEVEr) for proteomics Open
Extracellular vesicles (EVs), membrane-delimited nanovesicles that are secreted by cells into the extracellular environment, are gaining substantial interest due to their involvement in cellular homeostasis and their contribution to diseas…
View article: N-terminal proteoforms may engage in different protein complexes
N-terminal proteoforms may engage in different protein complexes Open
Alternative translation initiation and alternative splicing may give rise to N-terminal proteoforms, proteins that differ at their N-terminus compared with their canonical counterparts. Such proteoforms can have altered localizations, stab…
View article: N-terminal proteoforms may engage in different protein complexes
N-terminal proteoforms may engage in different protein complexes Open
Alternative translation initiation and alternative splicing may give rise to N-terminal proteoforms, proteins that differ at their N-terminus compared to their canonical counterparts. Such proteoforms can have altered localizations, stabil…
View article: Limited Evidence for Protein Products of Noncoding Transcripts in the HEK293T Cellular Cytosol
Limited Evidence for Protein Products of Noncoding Transcripts in the HEK293T Cellular Cytosol Open
Ribosome profiling has revealed translation outside canonical coding sequences, including translation of short upstream ORFs, long noncoding RNAs, overlapping ORFs, ORFs in UTRs, or ORFs in alternative reading frames. Studies combining mas…
View article: Limited, but potentially functional translation of non-coding transcripts in the HEK293T cellular cytosol
Limited, but potentially functional translation of non-coding transcripts in the HEK293T cellular cytosol Open
Ribosome profiling has revealed translation outside of canonical coding sequences (CDSs) including translation of short upstream ORFs, long non-coding RNAs, overlapping ORFs, ORFs in UTRs or ORFs in alternative reading frames. Studies comb…