Thomas A. Klar
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View article: Redox-Chemical Electron Storage on Sub-5 nm Gold Nanoparticles Compromised by Coulomb Repulsion
Redox-Chemical Electron Storage on Sub-5 nm Gold Nanoparticles Compromised by Coulomb Repulsion Open
?sterreichische Forschungsf?rderungsgesellschaft FO999888408
View article: Stimulated Emission Depletion Inspired Sub-100 nm Structuring of Epoxides Using 2-Chlorothioxanthone as Photosensitizer
Stimulated Emission Depletion Inspired Sub-100 nm Structuring of Epoxides Using 2-Chlorothioxanthone as Photosensitizer Open
Until very recently, the enhancement of multiphoton-based optical lithography by stimulated emission depletion (STED) inspired techniques was limited mostly to (meth)acrylates. Epoxides, which play an important role in semiconductor clean-…
View article: Multiphoton lithography with protein photoresists
Multiphoton lithography with protein photoresists Open
Recently, 2D/3D direct laser writing has attracted increased attention due to its broad applications ranging from biomedical engineering to aerospace. 3D nanolithography of water-soluble protein-based scaffolds have been envisioned to prov…
View article: New buffer systems for photopainting of single biomolecules
New buffer systems for photopainting of single biomolecules Open
We present novel buffer systems with paramagnetic cations and radical oxygen promoters which significantly enhance single-molecule LAPAP-efficiency due to specific cation-fluorophore interactions.
View article: STED-Inspired Cationic Photoinhibition Lithography
STED-Inspired Cationic Photoinhibition Lithography Open
Direct laser writing by two-photon lithography has been enhanced substantially during the past two decades by techniques borrowed from stimulated emission depletion (STED) microscopy. However, STED-inspired lithography was so far limited t…
View article: Low-Fluorescence Starter for Optical 3D Lithography of Sub-40 nm Structures
Low-Fluorescence Starter for Optical 3D Lithography of Sub-40 nm Structures Open
Stimulated emission depletion (STED) has been used to break the diffraction limit in fluorescence microscopy. Inspired by this success, similar methods were used to reduce the structure size in three-dimensional, subdiffractional optical l…
View article: Dual Channel Microfluidics for Mimicking the Blood–Brain Barrier
Dual Channel Microfluidics for Mimicking the Blood–Brain Barrier Open
High-resolution imaging is essential for analysis of the steps and way stations of cargo transport in in vitro models of the endothelium. In this study, we demonstrate a microfluidic system consisting of two channels horizontally separated…
View article: Plasmon-Assisted Direction- and Polarization-Sensitive Organic Thin-Film Detector
Plasmon-Assisted Direction- and Polarization-Sensitive Organic Thin-Film Detector Open
Utilizing Bragg surface plasmon polaritons (SPPs) on metal nanostructures for the use in optical devices has been intensively investigated in recent years. Here, we demonstrate the integration of nanostructured metal electrodes into an ITO…
View article: STED controlled photobleaching for sub-diffractional optical nanopatterning
STED controlled photobleaching for sub-diffractional optical nanopatterning Open
Laser-assisted protein adsorption by photobleaching (LAPAP) is a versatile tool to nanopattern proteins on the micrometer scale. Sub-micron patterning is, however, difficult due to diffraction. We show that, similar to stimulated emission …
View article: Localization STED (LocSTED) microscopy with 15 nm resolution
Localization STED (LocSTED) microscopy with 15 nm resolution Open
We present localization with stimulated emission depletion (LocSTED) microscopy, a combination of STED and single-molecule localization microscopy (SMLM). We use the simplest form of a STED microscope that is cost effective and synchroniza…
View article: Optical Coulomb blockade lifting in plasmonic nanoparticle dimers
Optical Coulomb blockade lifting in plasmonic nanoparticle dimers Open
If two metal nanoparticles are ultimately approached, a tunneling current prevents both an infinite redshift of the bonding dipolar plasmon and an infinite increase of the electric field in the hot spot between the nanoparticles. We argue …
View article: 3D multiphoton lithography using biocompatible polymers with specific mechanical properties
3D multiphoton lithography using biocompatible polymers with specific mechanical properties Open
Two new biocompatible polymers were designed, which can be 3D structured via multiphoton lithography. Their mechanical properties and biocompatibility were determined.
View article: Gold Nanoislands Grown on Multiphoton Polymerized Structures as Substrate for Enzymatic Reactions
Gold Nanoislands Grown on Multiphoton Polymerized Structures as Substrate for Enzymatic Reactions Open
We show that multiphoton thiol-ene polymerized structures comprise unreacted thiol moieties, which can be used for postpolymerization gold metallization. Some of the thiol groups located at the surface are not involved in thiol-ene reactio…
View article: Proteins on Supported Lipid Bilayers Diffusing around Proteins Fixed on Acrylate Anchors
Proteins on Supported Lipid Bilayers Diffusing around Proteins Fixed on Acrylate Anchors Open
Mobility of proteins and lipids plays a major role in physiological processes. Platforms which were developed to study protein interaction between immobilized and mobile proteins suffer from shortcomings such as fluorescence quenching or c…
View article: Biofunctionalization of Sub-Diffractionally Patterned Polymer Structures by Photobleaching
Biofunctionalization of Sub-Diffractionally Patterned Polymer Structures by Photobleaching Open
Stimulated emission depletion (STED) nanolithography allows nanofabrication below the diffraction limit. Recently, it was applied to nanoanchors for protein fixation down to the single molecule level. We now combined STED nanolithography w…
View article: Power Balance and Temperature in Optically Pumped Spasers and Nanolasers
Power Balance and Temperature in Optically Pumped Spasers and Nanolasers Open
Spasers and nanolasers produce a significant amount of heat, which impedes their realizability. We numerically investigate the farfield emission and thermal load in optically pumped spasers with a coupled electromagnetic/thermal model, inc…
View article: Photoacoustic microscopy of single cells employing an intensity-modulated diode laser
Photoacoustic microscopy of single cells employing an intensity-modulated diode laser Open
In this work, we employ frequency-domain photoacoustic microscopy to obtain photoacoustic images of labeled and unlabeled cells. The photoacoustic microscope is based on an intensity-modulated diode laser in combination with a focused piez…
View article: Multiphoton-Polymerized 3D Protein Assay
Multiphoton-Polymerized 3D Protein Assay Open
Multiphoton polymerization (MPP) enables 3D fabrication of micro- and nanoscale devices with complex geometries. Using MPP, we create a 3D platform for protein assays. Elevating the protein-binding sites above the substrate surface allows …
View article: Functional photoresists for sub-diffraction stimulated emission depletion lithography
Functional photoresists for sub-diffraction stimulated emission depletion lithography Open
Two novel photoresists were developed for the fabrication of sub-diffractionally sized polymeric nanostructures with chemically reactive surfaces. Using multiphoton polymerization as well as stimulated emission depletion (STED) lithography…
View article: Raman and Luminescent Spectra of Sulfonated Zn Phthalocyanine Enhanced by Gold Nanoparticles
Raman and Luminescent Spectra of Sulfonated Zn Phthalocyanine Enhanced by Gold Nanoparticles Open
Sulfonated Zn phthalocyanine, as a prospective photosensitizer in the photodynamic therapy of tumors, is investigated by means of Raman, infrared, and fluorescence spectroscopies. Conventional and surface-enhanced spectra from this photose…
View article: Bioinspired polymer microstructures for directional transport of oily liquids
Bioinspired polymer microstructures for directional transport of oily liquids Open
Nature has always served as an inspiration for scientists, helping them to solve a large diversity of technical problems. In our case, we are interested in the directional transport of oily liquids and as a model for this application we us…
View article: Bone‐forming cells with pronounced spread into the third dimension in polymer scaffolds fabricated by two‐photon polymerization
Bone‐forming cells with pronounced spread into the third dimension in polymer scaffolds fabricated by two‐photon polymerization Open
The main aim of this work was to stimulate bone‐forming cells to produce three‐dimensional networks of mineralized proteins such as those occurring in bones. This was achieved by a novel approach using a specific type of mesenchymal progen…
View article: Anticorrelation of Photoluminescence from Gold Nanoparticle Dimers with Hot-Spot Intensity
Anticorrelation of Photoluminescence from Gold Nanoparticle Dimers with Hot-Spot Intensity Open
Bulk gold shows photoluminescence (PL) with a negligible quantum yield of ∼10-10, which can be increased by orders of magnitude in the case of gold nanoparticles. This bears huge potential to use noble metal nanoparticles as fluorescent an…
View article: Frequency domain photoacoustic and fluorescence microscopy
Frequency domain photoacoustic and fluorescence microscopy Open
We report on simultaneous frequency domain optical-resolution photoacoustic and fluorescence microscopy with sub-µm lateral resolution. With the help of a blood smear, we show that photoacoustic and fluorescence images provide complementar…
View article: Plasmonic Nanostars as Efficient Broadband Scatterers for Random Lasing
Plasmonic Nanostars as Efficient Broadband Scatterers for Random Lasing Open
Huge spectral coverage of random lasing throughout the visible up to the infrared range is achieved with star-shaped gold nanoparticles ("nanostars"). As intrinsically broadband scattering centers, the nanostars are suspended in solutions …