Thomas P. Burghardt
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Neural-symbolic hybrid model for myosin complex in cardiac ventriculum decodes structural bases for inheritable heart disease from its genetic encoding Open
Background Human ventriculum myosin (βmys) powers contraction sometimes in complex with myosin binding protein C (MYBPC3). The latter regulates βmys activity and impacts overall cardiac function. Nonsynonymous single nucleotide variants (S…
Variants identify sarcomere inter-protein contacts distinguishing inheritable cardiac muscle diseases Open
Human ventriculum myosin (βmys) powers contraction sometimes while complexed with myosin binding protein C (MYBPC3) on the myosin thick filament. The latter regulates βmys activity through inter-protein contacts. Single nucleotide variants…
Natural variant frequencies across domains from different sarcomere proteins cross-correlate to identify inter-protein contacts associated with cardiac muscle function and disease Open
Coordinated sarcomere proteins produce contraction force for muscle shortening. In human ventriculum they include the cardiac myosin motor (βmys), repetitively converting ATP free energy into work, and myosin binding protein C (MYBPC3) tha…
Natural variants with 2D correlation genetics identify domains coordinating sarcomere proteins during contraction Open
Muscle proteins assemble in a sarcomere then by coordinated action produce contraction force to shorten muscle. In the human heart ventriculum, cardiac myosin motor (βmys) repetitively converts ATP free energy into work. Cardiac myosin bin…
The frequency-dependent effect of electrical fields on the mobility of intracellular vesicles in astrocytes Open
Slow-wave sleep, defined by low frequency (<4 Hz) electrical brain activity, is a basic brain function affecting metabolite clearance and memory consolidation. Although the origin of low-frequency activity is related to cortical up and dow…
the supplemental data of 'The frequency-dependent effect of electrical fields on the mobility of intracellular vesicles in astrocytes' Open
This data set is the supplemental data of the manuscript ‘The frequency-dependent effect of electrical fields on the mobility of intracellular vesicles in astrocytes’. Due to the size limitation of online storage, this data set contains pa…
the supplemental data of 'The frequency-dependent effect of electrical fields on the mobility of intracellular vesicles in astrocytes' Open
This data set is the supplemental data of the manuscript ‘The frequency-dependent effect of electrical fields on the mobility of intracellular vesicles in astrocytes’. Due to the size limitation of online storage, this data set contains pa…
Data for: Demographic Model for Inheritable Cardiac Disease Open
Four data sets for the fulfilled and unknown 6 dimensional data points from human cardiac: ventricular myosin (MYH7, MYL2, MYL3) , atrial myosin (MYH6, MYL4, MYL7), MYBPC3, and ACTC1
Demographic Model for Inheritable Cardiac Disease Open
The cardiac muscle proteins, generating and regulating energy transduction during a heartbeat, assemble in the sarcomere into a cyclical machine repetitively translating actin relative to myosin filaments. Myosin is the motor transducing A…
Cardiac and skeletal actin substrates uniquely tune cardiac myosin strain-dependent mechanics Open
Cardiac ventricular myosin (βmys) translates actin by transducing ATP free energy into mechanical work during muscle contraction. Unitary βmys translation of actin is the step-size. In vitro and in vivo βmys regulates contractile force and…
Supplementary Data: Uncured Pdms Inhibits Myosin In Vitro Motility In A Microfluidic Flow Cell Open
This is the supplementary data of manucript 'Uncured PDMS Inhibits Myosin In Vitro Motility in a Microfluidic Flow Cell'.
Supplementary Data: Uncured Pdms Inhibits Myosin In Vitro Motility In A Microfluidic Flow Cell Open
This is the supplementary data of manucript 'Uncured PDMS Inhibits Myosin In Vitro Motility in a Microfluidic Flow Cell'.
Uncured PDMS Inhibits Myosin In Vitro Motility in a Microfluidic Flow Cell Open
The myosin motor powers cardiac contraction and is frequently implicated in hereditary heart disease by its mutation. Principal motor function characteristics include myosin unitary step size, duty cycle, and force-velocity relationship fo…
Cardiac and Skeletal Actin Substrates Uniquely Tune Cardiac Myosin Strain-Dependent Mechanics Open
Native cardiac ventricular myosin (βmys) translates actin under load by transducing ATP free energy into mechanical work on actin during muscle contraction. Unitary βmys translation of actin is the myosin step-size. In vitro and in vivo βm…
Supplementary Data: Cardiac And Skeletal Actin Substrates Uniquely Tune Cardiac Myosin Strain-Dependent Mechanics Open
Supplementary data for the paper 'Cardiac and Skeletal Actin Substrates Uniquely Tune Cardiac Myosin Strain-Dependent Mechanics'.
Supplementary Data: Cardiac And Skeletal Actin Substrates Uniquely Tune Cardiac Myosin Strain-Dependent Mechanics Open
Supplementary data for the paper 'Cardiac and Skeletal Actin Substrates Uniquely Tune Cardiac Myosin Strain-Dependent Mechanics'.
Neural/Bayes network predictor for inheritable cardiac disease pathogenicity and phenotype Open
The cardiac muscle sarcomere contains multiple proteins contributing to contraction energy transduction and its regulation during a heartbeat. Inheritable heart disease mutants affect most of them but none more frequently than the ventricu…
Single cardiac ventricular myosins are autonomous motors Open
Myosin transduces ATP free energy into mechanical work in muscle. Cardiac muscle has dynamically wide-ranging power demands on the motor as the muscle changes modes in a heartbeat from relaxation, via auxotonic shortening, to isometric con…
Supplementary material from "Single cardiac ventricular myosins are autonomous motors" Open
Myosin transduces ATP free energy into mechanical work in muscle. Cardiac muscle has wide dynamically via ranging power demands on the motor as the muscle changes modes in a heartbeat from relaxation, auxotonic shortening, to isometric con…
Supplementary Data: Single Cardiac Ventricular Myosins Are Autonomous Motors Open
The files in this record contain partial data for the paper ‘Single Cardiac Ventricular Myosins are Autonomous Motors’. It includes one movie for unloaded Qdot data of WT, A57G and E143K, one movie for data at 3ug/ml of alpha-actinin, and …
Supplementary Data: Single Cardiac Ventricular Myosins Are Autonomous Motors Open
The files in this record contain partial data for the paper ‘Single Cardiac Ventricular Myosins are Autonomous Motors’. It includes one movie for unloaded Qdot data of WT, A57G and E143K, one movie for data at 3ug/ml of alpha-actinin, and …
Auxotonic to isometric contraction transitioning in a beating heart causes myosin step-size to down shift Open
Myosin motors in cardiac ventriculum convert ATP free energy to the work of moving blood volume under pressure. The actin bound motor cyclically rotates its lever-arm/light-chain complex linking motor generated torque to the myosin filamen…
Single Cardiac Ventricular Myosins Change Step-Size with Loading Open
The cardiac myosin motor powers the beating heart by catalyzed ATPase free energy conversion to contractile work. Transgenic mouse models for heart disease express mouse α-cardiac myosin heavy chain with human essential light chain (ELC) i…
Hypercontractile mutant of ventricular myosin essential light chain leads to disruption of sarcomeric structure and function and results in restrictive cardiomyopathy in mice Open
As a result of the E143K-induced myosin hypercontractility, the hearts of RCM mice model exhibited cardiac dysfunction, stiff ventricles and physiological, morphologic, and metabolic remodelling consistent with the development of RCM. Futu…
<i>In vivo</i> myosin step-size from zebrafish skeletal muscle Open
Muscle myosins transduce ATP free energy into actin displacement to power contraction. In vivo , myosin side chains are modified post-translationally under native conditions, potentially impacting function. Single myosin detection provides…