Thomas C. Ferrante
YOU?
Author Swipe
View article: Cytokines control the physical state of immune tissue
Cytokines control the physical state of immune tissue Open
Unlike most solid-like tissues, immune tissue is protean and reconfigurable—its component cells can patrol vast territories, find rare cellular partners, and dynamically self-organize into functional structures like germinal centers or ter…
View article: Induction of cervical dysfunction associated with preterm birth by IL-1 and dysbiotic microbiome revealed in human endocervix chips
Induction of cervical dysfunction associated with preterm birth by IL-1 and dysbiotic microbiome revealed in human endocervix chips Open
Cervical dysfunction, a major contributor to preterm labor and neonatal mortality, remains poorly understood due to the absence of physiologically relevant human models. Here, we show that a microfluidic human Endocervix Chip lined by prim…
View article: Organ chips with integrated multifunctional sensors enable continuous metabolic monitoring at controlled oxygen levels
Organ chips with integrated multifunctional sensors enable continuous metabolic monitoring at controlled oxygen levels Open
Despite remarkable advances in Organ-on-a-chip (Organ Chip) microfluidic culture technology, recreating tissue-relevant physiological conditions, such as the region-specific oxygen concentrations, remains a formidable technical challenge, …
View article: Organ Chips with integrated multifunctional sensors enable continuous metabolic monitoring at controlled oxygen levels
Organ Chips with integrated multifunctional sensors enable continuous metabolic monitoring at controlled oxygen levels Open
Despite remarkable advances in Organ-on-a-chip (Organ Chip) microfluidic culture technology, recreating tissue-relevant physiological conditions, such as the region-specific oxygen concentrations, remains a formidable technical challenge, …
View article: Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discovery
Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discovery Open
Organoids serve as a novel tool for disease modeling in three-dimensional multicellular contexts. Static organoids, however, lack the requisite biophysical microenvironment such as fluid flow, limiting their ability to faithfully recapitul…
View article: Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccination
Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccination Open
Multivalent presentation of ligands often enhances receptor activation and downstream signaling. DNA origami offers precise nanoscale spacing of ligands, a potentially useful feature for therapeutic nanoparticles. Here we introduce a “squa…
View article: Ectopic Lymphoid Follicle Formation and Human Seasonal Influenza Vaccination Responses Recapitulated in an Organ‐on‐a‐Chip (Adv. Sci. 14/2022)
Ectopic Lymphoid Follicle Formation and Human Seasonal Influenza Vaccination Responses Recapitulated in an Organ‐on‐a‐Chip (Adv. Sci. 14/2022) Open
Organ‐on‐a‐Chip A microfluidic organ‐on‐a‐chip model of the human lymphoid follicle that recapitulates vaccination responses in vitro. Blood‐derived cells self assemble into germinal centers in a matrix gel in the lower channel when nutrie…
View article: Ectopic Lymphoid Follicle Formation and Human Seasonal Influenza Vaccination Responses Recapitulated in an Organ‐on‐a‐Chip
Ectopic Lymphoid Follicle Formation and Human Seasonal Influenza Vaccination Responses Recapitulated in an Organ‐on‐a‐Chip Open
Lymphoid follicles (LFs) are responsible for generation of adaptive immune responses in secondary lymphoid organs and form ectopically during chronic inflammation. A human model of ectopic LF formation will provide a tool to understand LF …
View article: Increased energy demand from anabolic-catabolic processes drives β-lactam antibiotic lethality
Increased energy demand from anabolic-catabolic processes drives β-lactam antibiotic lethality Open
View article: Minimally instrumented SHERLOCK (miSHERLOCK) for CRISPR-based point-of-care diagnosis of SARS-CoV-2 and emerging variants
Minimally instrumented SHERLOCK (miSHERLOCK) for CRISPR-based point-of-care diagnosis of SARS-CoV-2 and emerging variants Open
An integrated, low-cost, sample-to-answer, CRISPR-based diagnostic detects SARS-CoV-2 and variants from unprocessed saliva.
View article: Saccharomyces cerevisiae Requires <i>CFF1</i> To Produce 4-Hydroxy-5-Methylfuran-3(2H)-One, a Mimic of the Bacterial Quorum-Sensing Autoinducer AI-2
Saccharomyces cerevisiae Requires <i>CFF1</i> To Produce 4-Hydroxy-5-Methylfuran-3(2H)-One, a Mimic of the Bacterial Quorum-Sensing Autoinducer AI-2 Open
Quorum sensing is a cell-to-cell communication process that bacteria use to monitor local population density. Quorum sensing relies on extracellular signal molecules called autoinducers (AIs).
View article: Barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel <i>in situ</i> analyses
Barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel <i>in situ</i> analyses Open
We present barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel insitu analyses (BOLORAMIS), a reverse transcription-free method for spatially-resolved, targeted, in situ RNA identification of single or multiple …
View article: <i>Saccharomyces cerevisiae</i>Requires<i>CFF1</i>to Produce 4-hydroxy-5-methylfuran-3(2H)-one, a Mimic of the Bacterial Quorum-Sensing Autoinducer AI-2
<i>Saccharomyces cerevisiae</i>Requires<i>CFF1</i>to Produce 4-hydroxy-5-methylfuran-3(2H)-one, a Mimic of the Bacterial Quorum-Sensing Autoinducer AI-2 Open
Quorum sensing is a process of cell-to-cell communication that bacteria use to orchestrate collective behaviors. Quorum sensing depends on the production, release, and detection of extracellular signal molecules called autoinducers (AIs) t…
View article: Robotic fluidic coupling and interrogation of multiple vascularized organ chips
Robotic fluidic coupling and interrogation of multiple vascularized organ chips Open
View article: Human Colon-on-a-Chip Enables Continuous In Vitro Analysis of Colon Mucus Layer Accumulation and Physiology
Human Colon-on-a-Chip Enables Continuous In Vitro Analysis of Colon Mucus Layer Accumulation and Physiology Open
View article: Human colon-on-a-chip enables continuous <i>in vitro</i> analysis of colon mucus layer accumulation and physiology
Human colon-on-a-chip enables continuous <i>in vitro</i> analysis of colon mucus layer accumulation and physiology Open
Background & Aims The mucus layer in the human colon protects against commensal bacteria and pathogens, and defects in its unique bilayered structure contribute to intestinal disorders, such as ulcerative colitis. However, our understandin…
View article: A robotic platform for fluidically-linked human body-on-chips experimentation
A robotic platform for fluidically-linked human body-on-chips experimentation Open
Here we describe of an ‘Interrogator’ instrument that uses liquid-handling robotics, a custom software package, and an integrated mobile microscope to enable automated culture, perfusion, medium addition, fluidic linking, sample collection…
View article: BioBits™ Bright: A fluorescent synthetic biology education kit
BioBits™ Bright: A fluorescent synthetic biology education kit Open
We report portable, user-friendly reagents and equipment for visual, hands-on biology activities with supporting curriculum.
View article: Barcoded oligonucleotides ligated on RNA amplified for multiplex and parallel in-situ analyses
Barcoded oligonucleotides ligated on RNA amplified for multiplex and parallel in-situ analyses Open
We present B arcoded O ligonucleotides L igated O n R NA A mplified for M ultiplexed and parallel I n- S itu analysis (BOLORAMIS), a reverse-transcription (RT)-free method for spatially-resolved, targeted, in-situ RNA identification of sin…
View article: Modeling radiation injury-induced cell death and countermeasure drug responses in a human Gut-on-a-Chip
Modeling radiation injury-induced cell death and countermeasure drug responses in a human Gut-on-a-Chip Open
View article: Fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissues v1
Fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissues v1 Open
RNA sequencing measures the quantitative change in gene expression over the whole transcriptome, but it lacks spatial context. On the other hand, in situ hybridization provides the location of gene expression, but only for a small number o…
View article: Mechanical induction of dentin-like differentiation by adult mouse bone marrow stromal cells using compressive scaffolds
Mechanical induction of dentin-like differentiation by adult mouse bone marrow stromal cells using compressive scaffolds Open
Tooth formation during embryogenesis is controlled through a complex interplay between mechanical and chemical cues. We have previously shown that physical cell compaction of dental mesenchyme cells during mesenchymal condensation is respo…
View article: Correction: Corrigendum: Photothermally triggered actuation of hybrid materials as a new platform for in vitro cell manipulation
Correction: Corrigendum: Photothermally triggered actuation of hybrid materials as a new platform for in vitro cell manipulation Open
View article: Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip
Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip Open
View article: Photothermally triggered actuation of hybrid materials as a new platform for in vitro cell manipulation
Photothermally triggered actuation of hybrid materials as a new platform for in vitro cell manipulation Open
Mechanical forces in the cell’s natural environment have a crucial impact on growth, differentiation and behaviour. Few areas of biology can be understood without taking into account how both individual cells and cell networks sense and tr…
View article: Photothermally triggered actuation of hybrid materials as a new platform for in vitro cell manipulation
Photothermally triggered actuation of hybrid materials as a new platform for in vitro cell manipulation Open
Mechanical forces in the cell’s natural environment have a crucial impact on growth, differentiation and behaviour. Few areas of biology can be understood without taking into account how both individual cells and cell networks sense and tr…
View article: Human Gut-On-A-Chip Supports Polarized Infection of Coxsackie B1 Virus In Vitro
Human Gut-On-A-Chip Supports Polarized Infection of Coxsackie B1 Virus In Vitro Open
Analysis of enterovirus infection is difficult in animals because they express different virus receptors than humans, and static cell culture systems do not reproduce the physical complexity of the human intestinal epithelium. Here, using …
View article: Matched-Comparative Modeling of Normal and Diseased Human Airway Responses Using a Microengineered Breathing Lung Chip
Matched-Comparative Modeling of Normal and Diseased Human Airway Responses Using a Microengineered Breathing Lung Chip Open
View article: Small airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitro
Small airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitro Open
View article: Bactericidal Antibiotics Induce Toxic Metabolic Perturbations that Lead to Cellular Damage
Bactericidal Antibiotics Induce Toxic Metabolic Perturbations that Lead to Cellular Damage Open