Thomas Dittmar
YOU?
Author Swipe
Hyaluronic acid promotes biomineralization of osteoblast-like cells – observations on two different barrier membranes Open
Background Guided bone regeneration (GBR) relies on biocompatible membranes to support osteogenesis. 1,4-butanediol diglycidyl ether (BDDE)-crosslinked hyaluronic acid (xHyA) has shown promise in enhancing bone regeneration, yet its mechan…
Differential Effects of Snail-KO in Human Breast Epithelial Cells and Human Breast Epithelial × Human Breast Cancer Hybrids Open
Snail and Zeb1 have been suggested as markers for the hybrid/mixed epithelial (E)/mesenchymal (M) state of cancer cells. Such cancer cells co-express E- and M-specific transcripts and possess cancer stem cell properties. M13HS-2/-8 tumor h…
Effects of polystyrene nano- and microplastics on human breast epithelial cells and human breast cancer cells Open
The continuous littering of the environment with plastic and the resulting nano- and microplastics produced from various processes are ever-increasing problems. These materials also affect humans, as the absorption and accumulation of nano…
How Much Do You Fuse? A Comparison of Cell Fusion Assays in a Breast Cancer Model Open
Cell fusion is a biological process that is crucial for the development and homeostasis of different tissues, but it is also pathophysiologically associated with tumor progression and malignancy. The investigation of cell fusion processes …
Altered Phenotypes of Breast Epithelial × Breast Cancer Hybrids after ZEB1 Knock-Out Open
ZEB1 plays a pivotal role in epithelial-to-mesenchymal transition (EMT), (cancer) cell stemness and cancer therapy resistance. The M13HS tumor hybrids, which were derived from spontaneous fusion events between the M13SV1-EGFP-Neo breast ep…
View article: Why do certain cancer cells alter functionality and fuse?
Why do certain cancer cells alter functionality and fuse? Open
Cancer cell fusion represents a rare event. However, the surviving cancer hybrid cells after a post-hybrid selection process (PHSP) can overgrow other cancer cells by exhibiting a proliferation advantage and/or expression of cancer stem-li…
Extracellular Events Involved in Cancer Cell–Cell Fusion Open
Fusion among different cell populations represents a rare process that is mediated by both intrinsic and extracellular events. Cellular hybrid formation is relayed by orchestrating tightly regulated signaling pathways that can involve both…
Impact of Cross-Linked Hyaluronic Acid on Osteogenic Differentiation of SAOS-2 Cells in an Air-Lift Model Open
The aim of this study was to investigate the impact of cross-linked hyaluronic acid on osteoblast-like cells seeded on top of two collagen substrates, native porcine pericardium membrane (substrate A) and ribose cross-linked collagen membr…
Modification of slow sand filtration of secondary effluents by electric fields: electrokinetics and influence factors Open
In this study, the effect of electric fields applied to a quartz sand bed on electrokinetics and the disinfection performance of an E-slow sand filter (reduction of Escherichia coli) were investigated. Results of electrokinetic experiments…
Human Gingival Fibroblast Adhesion and Proliferation on Hydroxyapatite-Coated Zirconia Abutment Surfaces Open
Applying antibacterial coatings to dental implant materials seems reasonable but can have negative influences on desired cell adhesion and healing. In this study, zirconia abutment specimens interacting with gingival tissue were used. The …
Generation of Cancer Stem/Initiating Cells by Cell–Cell Fusion Open
CS/ICs have raised great expectations in cancer research and therapy, as eradication of this key cancer cell type is expected to lead to a complete cure. Unfortunately, the biology of CS/ICs is rather complex, since no common CS/IC marker …
View article: A Human In Vitro Model to Study Adenoviral Receptors and Virus Cell Interactions
A Human In Vitro Model to Study Adenoviral Receptors and Virus Cell Interactions Open
To develop adenoviral cell- or tissue-specific gene delivery, understanding of the infection mechanisms of adenoviruses is crucial. Several adenoviral attachment proteins such as CD46, CAR and sialic acid have been identified and studied. …
Cell-Cell Fusion Mediated by Viruses and HERV-Derived Fusogens in Cancer Initiation and Progression Open
Cell fusion is a well-known, but still scarcely understood biological phenomenon, which might play a role in cancer initiation, progression and formation of metastases. Although the merging of two (cancer) cells appears simple, the entire …
The Role of MSCs and Cell Fusion in Tissue Regeneration Open
Regenerative medicine is concerned with the investigation of therapeutic agents that can be used to promote the process of regeneration after injury or in different diseases. Mesenchymal stem/stromal cells (MSCs) and their secretome—includ…
Cancer Cell Fusion and Post-Hybrid Selection Process (PHSP) Open
Fusion of cancer cells either with other cancer cells (homotypic fusion) in local vicinity of the tumor tissue or with other cell types (e.g., macrophages, cancer-associated fibroblasts (CAFs), mesenchymal stromal-/stem-like cells (MSC)) (…
Hybrid Formation and Fusion of Cancer Cells In Vitro and In Vivo Open
The generation of cancer hybrid cells by intra-tumoral cell fusion opens new avenues for tumor plasticity to develop cancer stem cells with altered properties, to escape from immune surveillance, to change metastatic behavior, and to broad…
Cell–Cell Fusion and the Roads to Novel Properties of Tumor Hybrid Cells Open
The phenomenon of cancer cell–cell fusion is commonly associated with the origin of more malignant tumor cells exhibiting novel properties, such as increased drug resistance or an enhanced metastatic capacity. However, the whole process of…
Cell Fusion of Mesenchymal Stem/Stromal Cells and Breast Cancer Cells Leads to the Formation of Hybrid Cells Exhibiting Diverse and Individual (Stem Cell) Characteristics Open
Cancer is one of the most common diseases worldwide, and treatment bears many challenges such as drug and radioresistance and formation of metastases. These difficulties are due to tumor heterogeneity, which has many origins. One may be ce…
Reversible Growth-Arrest of a Spontaneously-Derived Human MSC-Like Cell Line Open
Life cycle limitation hampers the production of high amounts of primary human mesenchymal stroma-/stem-like cells (MSC) and limits cell source reproducibility for clinical applications. The characterization of permanently growing MSC544 re…
Spectrum-Wide Exploration of Human Adenoviruses for Breast Cancer Therapy Open
Oncolytic adenoviruses (Ads) are promising tools for cancer therapeutics. However, most Ad-based therapies utilize Ad type 5 (Ad5), which displays unsatisfying efficiency in clinical trials, partly due to the low expression levels of its p…
Cell Fusion-Mediated Tissue Regeneration as an Inducer of Polyploidy and Aneuploidy Open
The biological phenomenon of cell fusion plays a crucial role in several physiological processes, including wound healing and tissue regeneration. Here, it is assumed that bone marrow-derived stem cells (BMSCs) could adopt the specific pro…
Minocycline impairs TNF-α-induced cell fusion of M13SV1-Cre cells with MDA-MB-435-pFDR1 cells by suppressing NF-κB transcriptional activity and its induction of target-gene expression of fusion-relevant factors Open
Minocycline is a potent inhibitor in the TNF-α-induced cell fusion process by targeting the NF-κB pathway. Thus, minocycline prevented NF-κB activation and nuclear translocation to abolish the target-gene expression of MMP9 and ICAM1 in M1…
Cell Fusion in Human Cancer: The Dark Matter Hypothesis Open
Current strategies to determine tumor × normal (TN)-hybrid cells among human cancer cells include the detection of hematopoietic markers and other mesodermal markers on tumor cells or the presence of donor DNA in cancer samples from patien…
Low-Pressure Plasma Sterilization for Test Specimens to be Worn on Splints in the Oral Cavity Open
Bacterial biofilms adhere to all oral surfaces and may alter or degrade them. For investigations of the oral biofilm, growing on new restorative dental biomaterials, sterilized dental enamel surfaces as natural, control, and reference mate…
Portable Analyzer for On-Site Determination of Dissolved Organic Carbon—Development and Field Testing Open
Dissolved organic carbon (DOC) is a sum parameter that is frequently used in water analytics. Highly resolved and accurate DOC data are necessary, for instance, for water quality monitoring and for the evaluation of the efficiency of treat…
PO-156 TNFα-induced cell fusion between MDA-MB435-pFDR.1 and M13SV1-Cre cells is suppressed by minocycline through inhibition of the NF-KB pathway Open
Introduction Cell fusion is involved in a wide range of physiological processes like fertilisation or wound healing. But also diseases could arise by cell fusion, such as cancer. In a cancer context, cell fusion generates hybrid cells, whi…