Thomas E. Clemente
YOU?
Author Swipe
View article: Large scale deletion and rebalancing within the k1C kafirin family in sorghum
Large scale deletion and rebalancing within the k1C kafirin family in sorghum Open
Sorghum bicolor (L.) Moench (sorghum) is cultivated as food for humans and livestock and is valued for its low input requirements. However, sorghum grain protein is deficient in the essential amino acid lysine and has poor protein digestib…
View article: Nitrogen response and growth trajectory of sorghum CRISPR-Cas9 mutants using high-throughput phenotyping
Nitrogen response and growth trajectory of sorghum CRISPR-Cas9 mutants using high-throughput phenotyping Open
Inorganic nitrogen (N) fertilizer has emerged as one of the key factors driving increased crop yields in the past several decades; however, the overuse of chemical N fertilizer has led to severe ecological and environmental burdens. Unders…
View article: Reducing stomatal density by expression of a synthetic EPF increases leaf intrinsic water use efficiency and reduces plant water use in a C<sub>4</sub>crop
Reducing stomatal density by expression of a synthetic EPF increases leaf intrinsic water use efficiency and reduces plant water use in a C<sub>4</sub>crop Open
Enhancing crop water use efficiency (WUE) is a key target trait for climatic resilience and expanding cultivation on marginal lands. Reducing stomatal conductance ( g s ) through manipulating stomatal density has been observed to translate…
View article: Plants having increased biomass and methods for making the same
Plants having increased biomass and methods for making the same Open
The impact of plastid size change in both monocot and dicot plants has been examined. In both, when plastid size is increased there is an increase in biomass relative to the parental lines. Thus, provided herein are methods for increasing …
View article: Large and stable genome edits at the sorghum alpha kafirin locus result in changes in chromatin accessibility and globally increased expression of genes encoding lysine enrichment
Large and stable genome edits at the sorghum alpha kafirin locus result in changes in chromatin accessibility and globally increased expression of genes encoding lysine enrichment Open
Introduction Sorghum is a resilient and widely cultivated grain crop used for feed and food. However, it’s grain is deficient in lysine, an essential amino acid. This is due to the primary seed storage proteins, the alpha-kafirins, lacking…
View article: Additional file 4 of Transformation and gene editing in the bioenergy grass Miscanthus
Additional file 4 of Transformation and gene editing in the bioenergy grass Miscanthus Open
Additional file 4. Sequence data for edits in lemon white1 in three Miscanthus species. Description: Aligned DNA sequence; format: FASTA.
View article: Additional file 2 of Transformation and gene editing in the bioenergy grass Miscanthus
Additional file 2 of Transformation and gene editing in the bioenergy grass Miscanthus Open
Additional file 2. Sequence and location of three gRNAs in the lemon white1 gene. Description: Multiple sequence alignments of lemon white1 orthologs from sorghum and miscanthus and the three gRNAs used to edit the gene; format: FASTA mult…
View article: Additional file 3 of Transformation and gene editing in the bioenergy grass Miscanthus
Additional file 3 of Transformation and gene editing in the bioenergy grass Miscanthus Open
Additional file 3. Sequence and detailed map of pHA194. Description: Sequence and annotation of all the parts in pHA194 plasmid; format: GenBank sequence format.
View article: Phenotypic Characterization of Sorghum Nitrogen Responsive Gene Edits
Phenotypic Characterization of Sorghum Nitrogen Responsive Gene Edits Open
Crop improvement over the last few decades, especially after the Green Revolution, is partially driven by the intensive application of less expensive inorganic nitrogen (N) fertilizer. However, the unsustainable use of inorganic N fertiliz…
View article: Transformation and gene editing in the bioenergy grass Miscanthus
Transformation and gene editing in the bioenergy grass Miscanthus Open
Background: Miscanthus , a C4 member of Poaceae, is a promising perennial crop for bioenergy, renewable bioproducts, and carbon sequestration. Species of interest include nothospecies M. x giganteus and its parental species M. sacchariflor…
View article: A phytobacterial TIR domain effector manipulates NAD <sup>+</sup> to promote virulence
A phytobacterial TIR domain effector manipulates NAD <sup>+</sup> to promote virulence Open
Summary The Pseudomonas syringae DC3000 type III effector HopAM1 suppresses plant immunity and contains a Toll/interleukin‐1 receptor (TIR) domain homologous to immunity‐related TIR domains of plant nucleotide‐binding leucine‐rich repeat r…
View article: Development of <i>mPing</i> ‐based activation tags for crop insertional mutagenesis
Development of <i>mPing</i> ‐based activation tags for crop insertional mutagenesis Open
Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To a…
View article: Development of<i>mPing</i>-based Activation Tags for Crop Insertional Mutagenesis
Development of<i>mPing</i>-based Activation Tags for Crop Insertional Mutagenesis Open
Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To a…
View article: The <i>LATERAL ROOT DENSITY</i> gene regulates root growth during water stress in wheat
The <i>LATERAL ROOT DENSITY</i> gene regulates root growth during water stress in wheat Open
Summary Drought stress is the major limiting factor in agriculture. Wheat, which is the most widely grown crop in the world, is predominantly cultivated in drought‐prone rainfed environments. Since roots play a critical role in water uptak…
View article: Genetic tool development in marine protists: Emerging model organisms for experimental cell biology
Genetic tool development in marine protists: Emerging model organisms for experimental cell biology Open
Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do n…
View article: Response of Sorghum Enhanced in Monolignol Biosynthesis to Stalk Rot Pathogens
Response of Sorghum Enhanced in Monolignol Biosynthesis to Stalk Rot Pathogens Open
To increase phenylpropanoid constituents and energy content in the versatile C 4 grass sorghum (Sorghum bicolor [L.] Moench), sorghum genes for proteins related to monolignol biosynthesis were overexpressed: SbMyb60 (transcriptional activa…
View article: Overexpression of the Sorghum bicolor SbCCoAOMT alters cell wall associated hydroxycinnamoyl groups
Overexpression of the Sorghum bicolor SbCCoAOMT alters cell wall associated hydroxycinnamoyl groups Open
Sorghum (Sorghum bicolor) is a drought tolerant crop, which is being developed as a bioenergy feedstock. The monolignol biosynthesis pathway is a major focus for altering the abundance and composition of lignin. Caffeoyl coenzyme-A O-methy…
View article: Engineering linear, branched‐chain triterpene metabolism in monocots
Engineering linear, branched‐chain triterpene metabolism in monocots Open
Summary Triterpenes are thirty‐carbon compounds derived from the universal five‐carbon prenyl precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Normally, triterpenes are synthesized via the mevalonate (MVA) pa…
View article: Editing of an Alpha-Kafirin Gene Family Increases, Digestibility and Protein Quality in Sorghum
Editing of an Alpha-Kafirin Gene Family Increases, Digestibility and Protein Quality in Sorghum Open
Kafirins are the major storage proteins in sorghum (Sorghum bicolor) grains and form protein bodies with poor digestibility. Since kafirins are devoid of the essential amino acid lysine, they also impart poor protein quality to the kernel.…
View article: METHOD FOR THE PRODUCTION OF HIGHSATURATED, LOW POLYUNSATURATEDSOYBEAN OIL
METHOD FOR THE PRODUCTION OF HIGHSATURATED, LOW POLYUNSATURATEDSOYBEAN OIL Open
Methods of genetically modifying soybean plants to alter the fatty acid properties of the oil are described.
View article: Overexpression of <i>SbMyb60</i> in <i>Sorghum bicolor</i> impacts both primary and secondary metabolism
Overexpression of <i>SbMyb60</i> in <i>Sorghum bicolor</i> impacts both primary and secondary metabolism Open
Summary Few transcription factors have been identified in C 4 grasses that either positively or negatively regulate monolignol biosynthesis. Previously, the overexpression of SbMyb60 in sorghum ( Sorghum bicolor ) has been shown to induce …
View article: Additional file 1: of Validation of QTL mapping and transcriptome profiling for identification of candidate genes associated with nitrogen stress tolerance in sorghum
Additional file 1: of Validation of QTL mapping and transcriptome profiling for identification of candidate genes associated with nitrogen stress tolerance in sorghum Open
Genetic distribution of SNPs discovered using genotyping-by-sequencing (GBS) in CK60/San Chi San RIL population. (XLSX 31Â kb)
View article: Additional file 5: of Validation of QTL mapping and transcriptome profiling for identification of candidate genes associated with nitrogen stress tolerance in sorghum
Additional file 5: of Validation of QTL mapping and transcriptome profiling for identification of candidate genes associated with nitrogen stress tolerance in sorghum Open
The list of common differentially expressed gene transcripts identified between CK60 and San Chi San, RIL bulks with high and low NUE using RNA-seq. (XLS 48Â kb)
View article: Additional file 3: of Validation of QTL mapping and transcriptome profiling for identification of candidate genes associated with nitrogen stress tolerance in sorghum
Additional file 3: of Validation of QTL mapping and transcriptome profiling for identification of candidate genes associated with nitrogen stress tolerance in sorghum Open
The list of differentially expressed gene transcripts identified between CK60 and San Chi San using RNA-seq. (XLSX 61Â kb)
View article: Additional file 2: of Validation of QTL mapping and transcriptome profiling for identification of candidate genes associated with nitrogen stress tolerance in sorghum
Additional file 2: of Validation of QTL mapping and transcriptome profiling for identification of candidate genes associated with nitrogen stress tolerance in sorghum Open
List of QTLs detected in CK60/China17 population that are overlapped with QTL intervals of CK60/San Chi San population. (XLSX 13Â kb)