Thomas E. Spratt
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View article: Activity of DNA polymerase κ across the genome in human fibroblasts
Activity of DNA polymerase κ across the genome in human fibroblasts Open
DNA polymerase κ (Polκ) is a specialized polymerase that has multiple cellular roles such as translesion DNA synthesis, replication of repetitive sequences, and nucleotide excision repair. We have developed a method for capturing DNA synth…
View article: Activity of DNA polymerase κ across the genome in human fibroblasts
Activity of DNA polymerase κ across the genome in human fibroblasts Open
DNA polymerase κ (Pol κ) is a specialized polymerase that has multiple cellular roles such as translesion DNA synthesis, replicating repetitive sequences, and nucleotide excision repair. We have developed a method for capturing DNA synthes…
View article: Zymo ChIP DNA Clean and Concentrator
Zymo ChIP DNA Clean and Concentrator Open
Various methods to isolate DNA
View article: Active Site Interactions Impact Phosphoryl Transfer during Replication of Damaged and Undamaged DNA by <i>Escherichia coli</i> DNA Polymerase I
Active Site Interactions Impact Phosphoryl Transfer during Replication of Damaged and Undamaged DNA by <i>Escherichia coli</i> DNA Polymerase I Open
Replicative DNA polymerases are able to discriminate between very similar substrates with high accuracy. One mechanism by which E. coli DNA polymerase I checks for Watson-Crick geometry is through a hydrogen bonding fork between Arg668 and…
View article: Mutagenic Replication of <i>N</i><sup>2</sup>-Deoxyguanosine Benzo[<i>a</i>]pyrene Adducts by <i>Escherichia coli</i> DNA Polymerase I and <i>Sulfolobus solfataricus</i> DNA Polymerase IV
Mutagenic Replication of <i>N</i><sup>2</sup>-Deoxyguanosine Benzo[<i>a</i>]pyrene Adducts by <i>Escherichia coli</i> DNA Polymerase I and <i>Sulfolobus solfataricus</i> DNA Polymerase IV Open
Benzo[a]pyrene, a potent human carcinogen, is metabolized in vivo to a diol epoxide that reacts with the N2-position of guanine to produce N2-BP-dG adducts. These adducts are mutagenic causing G to T transversions. These adducts block repl…
View article: N<sup>2</sup>‐Substituted 2′‐Deoxyguanosine Triphosphate Derivatives as Selective Substrates for Human DNA Polymerase κ
N<sup>2</sup>‐Substituted 2′‐Deoxyguanosine Triphosphate Derivatives as Selective Substrates for Human DNA Polymerase κ Open
N 2 ‐Alkyl‐2′‐deoxyguanosine triphosphate (N 2 ‐alkyl‐dGTP) derivatives with methyl, butyl, benzyl, or 4‐ethynylbenzyl substituents were prepared and tested as substrates for human DNA polymerases. N 2 ‐Benzyl‐dGTP was equal to dGTP as a s…
View article: DNA Polymerase ν Rapidly Bypasses <i>O</i><sup>6</sup>-Methyl-dG but Not <i>O</i><sup>6</sup>-[4-(3-Pyridyl)-4-oxobutyl-dG and <i>O</i><sup>2</sup>-Alkyl-dTs
DNA Polymerase ν Rapidly Bypasses <i>O</i><sup>6</sup>-Methyl-dG but Not <i>O</i><sup>6</sup>-[4-(3-Pyridyl)-4-oxobutyl-dG and <i>O</i><sup>2</sup>-Alkyl-dTs Open
4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a potent tobacco carcinogen that forms mutagenic DNA adducts including O6-methyl-2'-deoxyguanosine (O6-Me-dG), O6-[4-(3-pyridyl)-4-oxobut-1-yl]-dG (O6-POB-dG), O2-methylthymidine (O2-…
View article: DNA Polymerases η and ζ Combine to Bypass <i>O</i><sup>2</sup>-[4-(3-Pyridyl)-4-oxobutyl]thymine, a DNA Adduct Formed from Tobacco Carcinogens
DNA Polymerases η and ζ Combine to Bypass <i>O</i><sup>2</sup>-[4-(3-Pyridyl)-4-oxobutyl]thymine, a DNA Adduct Formed from Tobacco Carcinogens Open
4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and N'-nitrosonornicotine (NNN) are important human carcinogens in tobacco products. They are metabolized to produce a variety 4-(3-pyridyl)-4-oxobutyl (POB) DNA adducts including O(2)-[…