Thomas Gutsmann
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View article: Transmission of influenza is driven by weather conditions in Germany
Transmission of influenza is driven by weather conditions in Germany Open
Influenza seasonality is influenced by environmental and ecological conditions as well as evolutionary processes shaping the transmission potential of airborne Influenza A virus (IAV) particles and subsequent infectious disease. Considerin…
View article: From Membrane Composition to Antimicrobial Strategies: Experimental and Computational Approaches to AMP Design and Selectivity
From Membrane Composition to Antimicrobial Strategies: Experimental and Computational Approaches to AMP Design and Selectivity Open
The United Nations have committed to end the epidemics of communicable diseases by 2030 (SDG Target 3.3). In contrast with this ambition, the rise of Multi Drug Resistant (MDR) and Pan Drug Resistant (PDR) bacteria poses a threat of a retu…
View article: From Membrane Composition to Antimicrobial Strategies: Experimental and Computational Approaches to AMP Design and Selectivity
From Membrane Composition to Antimicrobial Strategies: Experimental and Computational Approaches to AMP Design and Selectivity Open
The United Nations have committed to end the epidemics of communicable diseases by 2030 (SDG Target 3.3). In contrast with this ambition, the rise of Multi Drug Resistant (MDR) and Pan Drug Resistant (PDR) bacteria poses a threat of a retu…
View article: From Membrane Composition to Antimicrobial Strategies: Experimental and Computational Approaches to AMP Design and Selectivity
From Membrane Composition to Antimicrobial Strategies: Experimental and Computational Approaches to AMP Design and Selectivity Open
The United Nations have committed to end the epidemics of communicable diseases by 2030 (SDG Target 3.3). In contrast with this ambition, the rise of Multi Drug Resistant (MDR) and Pan Drug Resistant (PDR) bacteria poses a threat of a retu…
View article: Cytoadhesion of <i>Plasmodium falciparum</i>‐Infected Red Blood Cells Changes the Expression of Cytokine‐, Histone‐ and Antiviral Protein‐Encoding Genes in Brain Endothelial Cells
Cytoadhesion of <i>Plasmodium falciparum</i>‐Infected Red Blood Cells Changes the Expression of Cytokine‐, Histone‐ and Antiviral Protein‐Encoding Genes in Brain Endothelial Cells Open
Malaria remains a significant global health problem, mainly due to Plasmodium falciparum , which is responsible for most fatal infections. Infected red blood cells (iRBCs) evade spleen clearance by adhering to endothelial cells (ECs), trig…
View article: From Membrane Composition to Antimicrobial Strategies: Experimental and Computational Approaches to AMP De- sign and Selectivity
From Membrane Composition to Antimicrobial Strategies: Experimental and Computational Approaches to AMP De- sign and Selectivity Open
The United Nations have committed to end the epidemics of communicable diseases by 2030 (SDG Target 3.3). In contrast with this ambition, the rise of Multi Drug Resistant (MDR) and Pan Drug Resistant (PDR) bacteria poses a threat of a retu…
View article: “Meet the IUPAB councilor”—Thomas Gutsmann
“Meet the IUPAB councilor”—Thomas Gutsmann Open
As one of the twelve newly elected councillors, it is my pleasure to provide a brief biographical sketch for the readers of Biophys. Rev. and the members of the Biophysical Societies. I have been actively involved in the German Biophysical…
View article: A synthetic peptide mimic kills Candida albicans and synergistically prevents infection
A synthetic peptide mimic kills Candida albicans and synergistically prevents infection Open
More than two million people worldwide are affected by life-threatening, invasive fungal infections annually. Candida species are the most common cause of nosocomial, invasive fungal infections and are associated with mortality rates above…
View article: Cytoadhesion of<i>Plasmodium falciparum</i>-infected red blood cells changes the expression of cytokine-, histone- and antiviral protein-encoding genes in brain endothelial cells
Cytoadhesion of<i>Plasmodium falciparum</i>-infected red blood cells changes the expression of cytokine-, histone- and antiviral protein-encoding genes in brain endothelial cells Open
Malaria remains a significant global health problem, mainly due to Plasmodium falciparum , which is responsible for most fatal infections. Infected red blood cells (iRBCs) evade spleen clearance by adhering to endothelial cells (ECs), trig…
View article: Secretion of the fungal toxin candidalysin is dependent on conserved precursor peptide sequences
Secretion of the fungal toxin candidalysin is dependent on conserved precursor peptide sequences Open
The opportunistic fungal pathogen Candida albicans damages host cells via its peptide toxin, candidalysin. Before secretion, candidalysin is embedded in a precursor protein, Ece1, which consists of a signal peptide, the precursor of candid…
View article: The mechanistic basis of the membrane‐permeabilizing activities of the virulence‐associated protein A (VapA) from <i>Rhodococcus equi</i>
The mechanistic basis of the membrane‐permeabilizing activities of the virulence‐associated protein A (VapA) from <i>Rhodococcus equi</i> Open
Pathogenic Rhodococcus equi release the virulence‐associated protein A (VapA) within macrophage phagosomes. VapA permeabilizes phagosome and lysosome membranes and reduces acidification of both compartments. Using biophysical techniques, w…
View article: Structure–function relationships underpin disulfide loop cleavage‐dependent activation of <i>Legionella pneumophila</i> lysophospholipase A <scp>PlaA</scp>
Structure–function relationships underpin disulfide loop cleavage‐dependent activation of <i>Legionella pneumophila</i> lysophospholipase A <span>PlaA</span> Open
Legionella pneumophila , the causative agent of a life‐threatening pneumonia, intracellularly replicates in a specialized compartment in lung macrophages, the Legionella ‐containing vacuole (LCV). Secreted proteins of the pathogen govern i…
View article: Influence of Membrane Asymmetry on OmpF Insertion, Orientation and Function
Influence of Membrane Asymmetry on OmpF Insertion, Orientation and Function Open
The effect of asymmetric membranes containing lipopolysaccharides (LPS) on the outer membrane protein F (OmpF) reconstitution, channel orientation, and antibiotic permeation across the outer membrane was investigated. After forming an asym…
View article: Structure-function relationships underpin disulfide loop cleavage-dependent activation of<i>Legionella pneumophila</i>lysophosholipase A PlaA
Structure-function relationships underpin disulfide loop cleavage-dependent activation of<i>Legionella pneumophila</i>lysophosholipase A PlaA Open
Legionella pneumophila, the causative agent of a life-threatening pneumonia, intracellularly replicates in a specialized compartment in lung macrophages, the Legionella -containing vacuole (LCV). Secreted proteins of the pathogen govern im…
View article: Update on Toxicological and Safety Pharmacological Profiling of the Anti-Infective and Anti-Inflammatory Peptide Pep19-2.5
Update on Toxicological and Safety Pharmacological Profiling of the Anti-Infective and Anti-Inflammatory Peptide Pep19-2.5 Open
We are grateful for the excellent help from Sabrina Groth and Kerstin Stephan, who performed several cytotoxicity tests and the FRET experiments.Furthermore, many peptide syntheses were performed by Rainer Bartels with
View article: Candidalysin Is the Hemolytic Factor of Candida albicans
Candidalysin Is the Hemolytic Factor of Candida albicans Open
Candida albicans produces an important virulence factor, the hypha-associated Ece1-derived secreted peptide toxin candidalysin, which is crucial for the establishment of mucosal and systemic infections. C. albicans has also long been known…
View article: Toxicological and Safety Pharmacological Profiling of the Anti-Infective and Anti-Inflammatory Peptide Pep19-2.5
Toxicological and Safety Pharmacological Profiling of the Anti-Infective and Anti-Inflammatory Peptide Pep19-2.5 Open
Aspidasept (Pep19-2.5) and its derivative Pep19-4LF (“Aspidasept II”) are anti-infective and anti-inflammatory synthetic polypeptides currently in development for application against a variety of moderate to severe bacterial infections tha…
View article: CD36—A Host Receptor Necessary for Malaria Parasites to Establish and Maintain Infection
CD36—A Host Receptor Necessary for Malaria Parasites to Establish and Maintain Infection Open
Plasmodium falciparum-infected erythrocytes (PfIEs) present P. falciparum erythrocyte membrane protein 1 proteins (PfEMP1s) on the cell surface, via which they cytoadhere to various endothelial cell receptors (ECRs) on the walls of human b…
View article: The ESX-4 substrates, EsxU and EsxT, modulate Mycobacterium abscessus fitness
The ESX-4 substrates, EsxU and EsxT, modulate Mycobacterium abscessus fitness Open
ESX type VII secretion systems are complex secretion machineries spanning across the mycobacterial membrane and play an important role in pathogenicity, nutrient uptake and conjugation. We previously reported the role of ESX-4 in modulatin…
View article: Design of Membrane Active Peptides Considering Multi-Objective Optimization for Biomedical Application
Design of Membrane Active Peptides Considering Multi-Objective Optimization for Biomedical Application Open
A multitude of membrane active peptides exists that divides into subclasses, such as cell penetrating peptides (CPPs) capable to enter eukaryotic cells or antimicrobial peptides (AMPs) able to interact with prokaryotic cell envelops. Pepti…
View article: Flagellin lysine methyltransferase FliB catalyzes a [4Fe-4S] mediated methyl transfer reaction
Flagellin lysine methyltransferase FliB catalyzes a [4Fe-4S] mediated methyl transfer reaction Open
The methyltransferase FliB posttranslationally modifies surface-exposed ɛ-N-lysine residues of flagellin, the protomer of the flagellar filament in Salmonella enterica (S . enterica) . Flagellin methylation, reported originally in 1959, wa…
View article: Mass Spectrometric Quantification of the Antimicrobial Peptide Pep19-2.5 with Stable Isotope Labeling and Acidic Hydrolysis
Mass Spectrometric Quantification of the Antimicrobial Peptide Pep19-2.5 with Stable Isotope Labeling and Acidic Hydrolysis Open
Sepsis is the number one cause of death in intensive care units. This life-threatening condition is caused by bacterial infections and triggered by endotoxins of Gram-negative bacteria that leads to an overreaction of the immune system. Th…
View article: Cathelicidin and PMB neutralize endotoxins by multifactorial mechanisms including LPS interaction and targeting of host cell membranes
Cathelicidin and PMB neutralize endotoxins by multifactorial mechanisms including LPS interaction and targeting of host cell membranes Open
Significance Antibiotic resistance among clinically relevant bacteria presents an increasing threat and raises the urgent need for new compounds. Antimicrobial peptides and peptide antibiotics are potent membrane-active molecules and valua…
View article: Anti-Infective and Anti-Inflammatory Mode of Action of Peptide 19-2.5
Anti-Infective and Anti-Inflammatory Mode of Action of Peptide 19-2.5 Open
The polypeptide Pep19-2.5 (Aspidasept®) has been described to act efficiently against infection-inducing bacteria by binding and neutralizing their most potent toxins, i.e., lipopolysaccharides (LPS) and lipoproteins/peptides (LP), indepen…
View article: Flagellin lysine methyltransferase FliB catalyzes a [4Fe-4S] mediated methyl transfer reaction
Flagellin lysine methyltransferase FliB catalyzes a [4Fe-4S] mediated methyl transfer reaction Open
The methyltransferase FliB posttranslationally modifies surface-exposed ɛ-N-lysine residues of flagellin, the protomer of the flagellar filament in Salmonella enterica (S. enterica). Flagellin methylation, reported originally in 1959, was …
View article: Specific localisation of ions in bacterial membranes unravels physical mechanism of effective bacteria killing by sanitiser
Specific localisation of ions in bacterial membranes unravels physical mechanism of effective bacteria killing by sanitiser Open
Antimicrobial resistance is a major threat to public health. Although many commercial sanitisers contain a combination of cationic surfactants and aromatic alcohols, the physical mechanisms where these two substances bind to or how they di…
View article: The Beauty of Asymmetric Membranes: Reconstitution of the Outer Membrane of Gram-Negative Bacteria
The Beauty of Asymmetric Membranes: Reconstitution of the Outer Membrane of Gram-Negative Bacteria Open
The architecture of the lipid matrix of the outer membrane of Gram-negative bacteria is extremely asymmetric: Whereas the inner leaflet is composed of a phospholipid mixture, the outer leaflet is built up by glycolipids. For most Gram-nega…