Thomas Heitkamp
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View article: Protein succinylome analysis identifies citrate synthase as a central regulator of osteoclast metabolic activity
Protein succinylome analysis identifies citrate synthase as a central regulator of osteoclast metabolic activity Open
Tumour necrosis factor ligand superfamily member 11 (TNFSF11; RANKL) and macrophage colony‐stimulating factor 1 receptor (M‐CSF) differentiate macrophages into osteoclasts. This process is characterised by changes in metabolic activity tha…
View article: Mechanism of ADP-Inhibited ATP Hydrolysis in Single Proton-Pumping FoF1-ATP Synthase Trapped in Solution
Mechanism of ADP-Inhibited ATP Hydrolysis in Single Proton-Pumping FoF1-ATP Synthase Trapped in Solution Open
FoF1-ATP synthases in mitochondria, in chloroplasts, and in most bacteria are proton-driven membrane enzymes that supply the cells with ATP made from ADP and phosphate. Different control mechanisms exist to monitor and prevent the enzymes’…
View article: Mechanism of ADP-inhibited ATP hydrolysis in single proton-pumping F<sub>o</sub>F<sub>1</sub>-ATP synthase trapped in solution
Mechanism of ADP-inhibited ATP hydrolysis in single proton-pumping F<sub>o</sub>F<sub>1</sub>-ATP synthase trapped in solution Open
F o F 1 -ATP synthases in mitochondria, in chloroplasts and in most bacteria are the proton-driven membrane enzymes supplying the cells with ATP made from ADP and phosphate. To monitor and prevent the reverse chemical reaction of fast wast…
View article: Monitoring oligomerization dynamics of individual human neurotensin receptors 1 in living cells and in SMALP nanodiscs
Monitoring oligomerization dynamics of individual human neurotensin receptors 1 in living cells and in SMALP nanodiscs Open
The human neurotensin receptor 1 (NTSR1) is a G protein-coupled receptor. The receptor is activated by a small peptide ligand neurotensin. NTSR1 can be expressed in HEK cells by stable transfection. Previously we used the fluorescent prote…
View article: Monitoring oligomerization dynamics of individual human neurotensin receptors 1 in living cells and in SMALP nanodiscs
Monitoring oligomerization dynamics of individual human neurotensin receptors 1 in living cells and in SMALP nanodiscs Open
The human neurotensin receptor 1 (NTSR1) is a G protein-coupled receptor. The receptor is activated by a small peptide ligand neurotensin. NTSR1 can be expressed in HEK cells by stable transfection. Previously we used the fluorescent prote…
View article: Fast ATP-dependent Subunit Rotation in Reconstituted F<sub>o</sub>F<sub>1</sub>-ATP Synthase Trapped in Solution
Fast ATP-dependent Subunit Rotation in Reconstituted F<sub>o</sub>F<sub>1</sub>-ATP Synthase Trapped in Solution Open
F o F 1 -ATP synthases are ubiquitous membrane-bound, rotary motor enzymes that can catalyze ATP synthesis and hydrolysis. Their enzyme kinetics are controlled by internal subunit rotation, by substrate and product concentrations, by mecha…
View article: Fast ATP-dependent Subunit Rotation in Reconstituted FoF1-ATP Synthase Trapped in Solution
Fast ATP-dependent Subunit Rotation in Reconstituted FoF1-ATP Synthase Trapped in Solution Open
FoF1-ATP synthases are ubiquitous membrane-bound, rotary motor enzymes that can catalyze ATP synthesis and hydrolysis. Their enzyme kinetics are controlled by internal subunit rotation, by substrate and product concentrations, by mechanica…
View article: Light‐Driven ATP Regeneration in Diblock/Grafted Hybrid Vesicles
Light‐Driven ATP Regeneration in Diblock/Grafted Hybrid Vesicles Open
Light‐driven ATP regeneration systems combining ATP synthase and bacteriorhodopsin have been proposed as an energy supply in the field of synthetic biology. Energy is required to power biochemical reactions within artificially created reac…
View article: Observing monomer - dimer transitions of neurotensin receptors 1 in single SMALPs by homoFRET and in an ABELtrap
Observing monomer - dimer transitions of neurotensin receptors 1 in single SMALPs by homoFRET and in an ABELtrap Open
G protein-coupled receptors (GPCRs) are a large superfamily of membrane proteins that are activated by extracellular small molecules or photons. Neurotensin receptor 1 (NTSR1) is a GPCR that is activated by neurotensin, i.e. a 13 amino aci…
View article: Ligand-induced oligomerization of the human GPCR neurotensin receptor 1 monitored in living HEK293T cells
Ligand-induced oligomerization of the human GPCR neurotensin receptor 1 monitored in living HEK293T cells Open
The human neurotensin receptor 1 (NTSR1) is a G protein-coupled receptor that can be expressed in HEK293T cells by stable transfection. Its ligand is a 13-amino-acid peptide that binds with nanomolar affinity from the extracellular side to…
View article: Towards monitoring conformational changes of the GPCR neurotensin receptor 1 by single-molecule FRET
Towards monitoring conformational changes of the GPCR neurotensin receptor 1 by single-molecule FRET Open
Neurotensin receptor 1 (NTSR1) is a G protein-coupled receptor that is important for signaling in the brain and the gut. Its agonist ligand neurotensin (NTS), a 13-amino-acid peptide, binds with nanomolar affinity from the extracellular si…
View article: Imaging cytochrome C oxidase and FoF1-ATP synthase in mitochondrial cristae of living human cells by FLIM and superresolution microscopy
Imaging cytochrome C oxidase and FoF1-ATP synthase in mitochondrial cristae of living human cells by FLIM and superresolution microscopy Open
Cytochrome C oxidase and FoF1-ATP synthase constitute complex IV and V, respectively, of the five membrane-bound enzymes in mitochondria comprising the respiratory chain. These enzymes are located in the inner mitochondrial membrane (IMM),…
View article: Imaging cytochrome C oxidase and FoF1-ATP synthase in mitochondrial\n cristae of living human cells by FLIM and superresolution microscopy
Imaging cytochrome C oxidase and FoF1-ATP synthase in mitochondrial\n cristae of living human cells by FLIM and superresolution microscopy Open
Cytochrome C oxidase and FoF1-ATP synthase constitute complex IV and V,\nrespectively, of the five membrane-bound enzymes in mitochondria comprising the\nrespiratory chain. These enzymes are located in the inner mitochondrial\nmembrane (IM…
View article: Confining Brownian motion of single nanoparticles in an ABELtrap
Confining Brownian motion of single nanoparticles in an ABELtrap Open
Trapping nanoscopic objects to observe their dynamic behaviour for extended periods of time is an ongoing quest. Particularly, sub-100nm transparent objects are hard to catch and most techniques rely on immobilisation or transient diffusio…
View article: Observing single F<sub>o</sub>F<sub>1</sub>-ATP synthase at work using an improved fluorescent protein mNeonGreen as FRET donor
Observing single F<sub>o</sub>F<sub>1</sub>-ATP synthase at work using an improved fluorescent protein mNeonGreen as FRET donor Open
Adenosine triphosphate (ATP) is the universal chemical energy currency for cellular activities provided mainly by the membrane enzyme FoF1-ATP synthase in bacteria, chloroplasts and mitochondria. Synthesis of ATP is accompanied by subunit …