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View article: Peroxisome protein import deficiency causes heart failure in mouse and human
Peroxisome protein import deficiency causes heart failure in mouse and human Open
Peroxisomes are ubiquitous cellular organelles with potentially vital roles in lipid and reactive oxygen metabolism. The metabolic demands of the heart are substantial; however, the contribution of peroxisomes to cardiac development, healt…
View article: Offsetting ROS-mediated arrest of endothelial fenestration dynamics permits long-term optical super-resolution- and AFM-imaging
Offsetting ROS-mediated arrest of endothelial fenestration dynamics permits long-term optical super-resolution- and AFM-imaging Open
Advances in cell biology demand methods that resolve the structure and dynamics of subcellular organelles in living cells. Live-cell super-resolution fluorescence microscopy meets this need but is constrained by phototoxicity, which distur…
View article: Mimicking the Liver Sinusoidal Endothelial Cell Niche In Vitro to Enhance Fenestration in a Genetic Model of Systemic Inflammation
Mimicking the Liver Sinusoidal Endothelial Cell Niche In Vitro to Enhance Fenestration in a Genetic Model of Systemic Inflammation Open
Liver sinusoidal endothelial cells (LSECs) play a crucial role in hepatic homeostasis, clearance, and microcirculatory regulation. Their fenestrations—patent transcellular pores—are essential for proper liver function, yet disappear in pat…
View article: Live cell optical super-resolution microscopy of dystroglycan mutants as a model for dystroglycanopathies in multiple cell lines
Live cell optical super-resolution microscopy of dystroglycan mutants as a model for dystroglycanopathies in multiple cell lines Open
Introduction Dystroglycan (DG) is an adhesion complex comprising two subunits, α-DG and β-DG, which interact non-covalently at the plasma membrane. As a component of the dystrophin-glycoprotein complex DGC, DG plays a crucial role in linki…
View article: Super-resolution Live-cell Fluorescence Lifetime Imaging
Super-resolution Live-cell Fluorescence Lifetime Imaging Open
Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM …
View article: Hydrogen peroxide damage to rat liver sinusoidal endothelial cells is prevented by n-acetyl-cysteine but not GSH
Hydrogen peroxide damage to rat liver sinusoidal endothelial cells is prevented by n-acetyl-cysteine but not GSH Open
Background: Reactive oxygen species (ROS) are prevalent in the liver during intoxication, infection, inflammation, and aging. Changes in liver sinusoidal endothelial cells (LSEC) are associated with various liver diseases. Methods: Isolate…
View article: Correlative Super‐Resolution Imaging of Cellular Nanopores Facilitated by Transparent Polymer Waveguide Chips
Correlative Super‐Resolution Imaging of Cellular Nanopores Facilitated by Transparent Polymer Waveguide Chips Open
Super‐resolution optical microscopy (SRM) permits the visualization of subcellular structures of biological samples beyond the diffraction limit of light. To evaluate and utilize the specific strengths of each SRM technique a combined appr…
View article: Active axial motion compensation in multiphoton-excited fluorescence microscopy
Active axial motion compensation in multiphoton-excited fluorescence microscopy Open
In living organisms, the natural motion caused by heartbeat, breathing, or muscle movements leads to the deformation of tissue caused by translation and stretching of the tissue structure. This effect results in the displacement or deforma…
View article: Impact of oxidized low-density lipoprotein on rat liver sinusoidal endothelial cell morphology and function
Impact of oxidized low-density lipoprotein on rat liver sinusoidal endothelial cell morphology and function Open
Atherogenesis is associated with elevated plasma levels of oxidized low-density lipoproteins (oxLDL). In vivo, oxLDL causes liver endothelial swelling, and disrupts liver sinusoidal endothelial cell (LSECs) fenestrations. We mapped the nan…
View article: Super-resolution upgrade for deep tissue imaging featuring easy implementation
Super-resolution upgrade for deep tissue imaging featuring easy implementation Open
Deep tissue imaging with high contrast close to or even below the optical resolution limit is still challenging due to optical aberrations and scattering introduced by dense biological samples. This results in high complexity and cost of m…
View article: Image restoration in frequency space using complex-valued CNNs
Image restoration in frequency space using complex-valued CNNs Open
Real-valued convolutional neural networks (RV-CNNs) in the spatial domain have outperformed classical approaches in many image restoration tasks such as image denoising and super-resolution. Fourier analysis of the results produced by thes…
View article: Hydrogen peroxide damage to scavenging function and ultrastructure of liver sinusoidal endothelial cells is prevented by n-acetyl-cysteine but not GSH
Hydrogen peroxide damage to scavenging function and ultrastructure of liver sinusoidal endothelial cells is prevented by n-acetyl-cysteine but not GSH Open
Reactive oxygen species (ROS) are prevalent in the liver during intoxication, infection, inflammation, and ageing. Changes in liver sinusoidal endothelial cells (LSECs) are associated with various liver diseases. We investigated how oxidat…
View article: Two-photon line-scanning structured illumination microscopy combined with lightsheet scanning mode for super-resolution imaging in deep tissue
Two-photon line-scanning structured illumination microscopy combined with lightsheet scanning mode for super-resolution imaging in deep tissue Open
Imaging structures deep within biological tissue and organisms with a spatial resolution well below the optical diffraction limit is still underrepresented. This is mostly due to the complexity and high cost of microscopes that can facilit…
View article: High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy
High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy Open
High-resolution optical fluorescence microscopies and, in particular, super-resolution fluorescence microscopy, are rapidly adopting highly sensitive cameras as their referred photodetectors. Camera-based parallel detection facilitates hig…
View article: Watt-level all polarization-maintaining femtosecond fiber laser source at 1100 nm
Watt-level all polarization-maintaining femtosecond fiber laser source at 1100 nm Open
We demonstrate a compact watt-level all polarization-maintaining (PM) femtosecond fiber laser source at 1100 nm. The fiber laser source is seeded by an all PM fiber mode-locked laser employing a nonlinear amplifying loop mirror. The seed l…
View article: Uptake of microplastics and impacts on plant traits of savoy cabbage
Uptake of microplastics and impacts on plant traits of savoy cabbage Open
Anthropogenic influences such as plastic pollution are causing serious environmental problems. While effects of microplastics on marine organisms are well studied, less is known about effects of plastic particles on terrestrial organisms s…
View article: Watt-level all polarization-maintaining femtosecond fiber laser source at 1100 nm for multicolor two-photon fluorescence excitation of fluorescent proteins
Watt-level all polarization-maintaining femtosecond fiber laser source at 1100 nm for multicolor two-photon fluorescence excitation of fluorescent proteins Open
We demonstrate a compact watt-level all polarization-maintaining (PM) femtosecond fiber laser source at 1100 nm. The fiber laser source is seeded by an all PM fiber mode-locked laser employing a nonlinear amplifying loop mirror. The seed l…
View article: Active Axial Motion Compensation in Multiphoton-Excited Fluorescence Microscopy
Active Axial Motion Compensation in Multiphoton-Excited Fluorescence Microscopy Open
In living organisms, the natural motion caused by the heartbeat, breathing, or muscle movements leads to the deformation of tissue caused by translation and stretching of the tissue structure. This effect results in the displacement or def…
View article: Evaluation of Swin Transformer and knowledge transfer for denoising of super-resolution structured illumination microscopy data
Evaluation of Swin Transformer and knowledge transfer for denoising of super-resolution structured illumination microscopy data Open
Background Convolutional neural network (CNN)–based methods have shown excellent performance in denoising and reconstruction of super-resolved structured illumination microscopy (SR-SIM) data. Therefore, CNN-based architectures have been t…
View article: Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope
Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope Open
In super-resolution structured illumination microscopy (SR-SIM) the separation between opposing laser spots in the back focal plane of the objective lens affects the pattern periodicity, and, thus, the resulting spatial resolution. Here, w…
View article: Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser
Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser Open
Coherent Raman scattering (CRS) microscopy is an attractive label‐free imaging method providing high chemical sensitivity, sub‐micron spatial resolution, and video‐rate imaging speed. When extended to hyperspectral CRS (HS‐CRS), it is capa…
View article: Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope
Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope Open
In super-resolution structured illumination microscopy (SR-SIM) the separation between opposing laser spots in the back focal plane of the objective lens affects the pattern periodicity, and, thus, the resulting spatial resolution. Here, w…
View article: Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope
Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope Open
In super-resolution structured illumination microscopy (SR-SIM) the separation between opposing laser spots in the back focal plane of the objective lens affects the pattern periodicity, and, thus, the resulting spatial resolution. Here, w…
View article: Raw data accompanying the manuscript "Uptake of microplastics and impacts on plant traits of savoy cabbage"
Raw data accompanying the manuscript "Uptake of microplastics and impacts on plant traits of savoy cabbage" Open
These are the raw datasets used to generate Figure 1 for the manuscript entitled "Uptake of microplastics and impacts on plant traits of savoy cabbage". The folders contain all raw CARS and SRS data that are needed to reproduce Figure 1 (T…
View article: Raw data accompanying the manuscript "Uptake of microplastics and impacts on plant traits of savoy cabbage"
Raw data accompanying the manuscript "Uptake of microplastics and impacts on plant traits of savoy cabbage" Open
These are the raw datasets used to generate Figure 1 for the manuscript entitled "Uptake of microplastics and impacts on plant traits of savoy cabbage". The folders contain all raw CARS and SRS data that are needed to reproduce Figure 1 (T…
View article: High-speed TIRF and 2D super-resolution structured illumination microscopy with a large field of view based on fiber optic components
High-speed TIRF and 2D super-resolution structured illumination microscopy with a large field of view based on fiber optic components Open
Super-resolved structured illumination microscopy (SR-SIM) is among the most flexible, fast, and least perturbing fluorescence microscopy techniques capable of surpassing the optical diffraction limit. Current custom-built instruments are …