Thomas Kellerer
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View article: Speed-Up Phase Resolved Fluorescence Lifetime Imaging Microscopy (SUPER-FLIM) for Real-Time Microenvironmental Sensing
Speed-Up Phase Resolved Fluorescence Lifetime Imaging Microscopy (SUPER-FLIM) for Real-Time Microenvironmental Sensing Open
Imaging cell biological processes while simultaneously sensing the microenvironment at high spatial and temporal resolution is a key goal in modern live-cell microscopy. However, adding parameters such as fluorescence lifetime compromises …
View article: Super-resolution upgrade for deep tissue imaging featuring easy implementation
Super-resolution upgrade for deep tissue imaging featuring easy implementation Open
Deep tissue imaging with high contrast close to or even below the optical resolution limit is still challenging due to optical aberrations and scattering introduced by dense biological samples. This results in high complexity and cost of m…
View article: Two-photon line-scanning structured illumination microscopy combined with lightsheet scanning mode for super-resolution imaging in deep tissue
Two-photon line-scanning structured illumination microscopy combined with lightsheet scanning mode for super-resolution imaging in deep tissue Open
Imaging structures deep within biological tissue and organisms with a spatial resolution well below the optical diffraction limit is still underrepresented. This is mostly due to the complexity and high cost of microscopes that can facilit…
View article: Two-photon microscopy of acoustofluidic trapping for highly sensitive cell analysis
Two-photon microscopy of acoustofluidic trapping for highly sensitive cell analysis Open
Two-photon microscopy and acoustofluidics for 3D cell study close to living conditions, precise imaging, and real-time osmosis monitoring.
View article: Comprehensive Investigation of Parameters Influencing Fluorescence Lifetime Imaging Microscopy in Frequency- and Time-Domain Illustrated by Phasor Plot Analysis
Comprehensive Investigation of Parameters Influencing Fluorescence Lifetime Imaging Microscopy in Frequency- and Time-Domain Illustrated by Phasor Plot Analysis Open
Having access to fluorescence lifetime, researchers can reveal in-depth details about the microenvironment as well as the physico-chemical state of the molecule under investigation. However, the high number of influencing factors might be …
View article: Engineering Principles and Algorithmic Design Synthesis for Ultracompact Bio-Hybrid Perfusion Chip
Engineering Principles and Algorithmic Design Synthesis for Ultracompact Bio-Hybrid Perfusion Chip Open
Bioinspired 3D microfluidic systems that combine vascularization with extracellular matrix architectures of organotypic geometry, composition and biophysical traits can help advance our understanding of microorgan physiology. Here, two-pho…
View article: Correlative two-color two-photon (2C2P) excitation STED microscopy
Correlative two-color two-photon (2C2P) excitation STED microscopy Open
We present a two-color two-photon stimulated emission depletion microscopy technique (2C2P-STED) that correlates a confocal image with a super-resolved image employing the inherent self-referencing mechanism of nonlinear excitation. The no…