Thomas Kræmer
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View article: Fatal intoxication involving amphetamine, clonazafone, a benzodiazepine prodrug, and fluoro-etonitazene, a new synthetic opioid
Fatal intoxication involving amphetamine, clonazafone, a benzodiazepine prodrug, and fluoro-etonitazene, a new synthetic opioid Open
Intoxication cases involving new psychoactive substances (NPS) are known to provide various challenges for forensic toxicological case interpretation, starting with the identification of previously unknown substances. Furthermore, the phar…
View article: Prolonged Detection of GHB Intake in Urine: Are We Finally There?
Prolonged Detection of GHB Intake in Urine: Are We Finally There? Open
Gamma‐hydroxybutyric acid (GHB) has been implicated in drug‐facilitated sexual assault (DFSA). However, due to its rapid elimination and the challenge of distinguishing exogenous from endogenous levels, GHB is likely to be significantly un…
View article: New Approaches To Identify Urine and Hair Adulteration Attempts in Forensic Toxicology: A Proof-of-Concept Study Using a Proteomics Approach Based on Liquid Chromatography–Mass Spectrometry (LC-MS)
New Approaches To Identify Urine and Hair Adulteration Attempts in Forensic Toxicology: A Proof-of-Concept Study Using a Proteomics Approach Based on Liquid Chromatography–Mass Spectrometry (LC-MS) Open
Urine and hair are among the primary biological matrices used for drug and abstinence testing in clinical, forensic, and antidoping settings. A persistent challenge in such analyses is the adulteration of samples: through dilution, substit…
View article: Endocannabinoid and steroid hormone levels during and after pregnancy in fingernail samples from mothers and their infants
Endocannabinoid and steroid hormone levels during and after pregnancy in fingernail samples from mothers and their infants Open
Stress exposure and protective biological factors such as steroid hormones and endocannabinoids (eCBs) play a crucial role in pregnancy and birth outcomes. The keratinized nail matrix is a convenient medium for long-term hormone monitoring…
View article: Evaluation of Therapeutic Opioids in Hair of Neonatal and Pediatric Patients
Evaluation of Therapeutic Opioids in Hair of Neonatal and Pediatric Patients Open
Forensic hair analysis poses a valuable tool for assessing opioid exposure in children and neonates. However, reliable literature data on opioid concentrations in the hair of this population are mostly scarce, making the interpretation of …
View article: Toward a Cannabis Terroir: Untargeted Metabolomic Profiling of Authentic Samples Using Gas Chromatography–High‐Resolution Mass Spectrometry (GC‐HRMS) and Liquid Chromatography–High‐Resolution Tandem Mass Spectrometry (LC‐HRMS/MS)
Toward a Cannabis Terroir: Untargeted Metabolomic Profiling of Authentic Samples Using Gas Chromatography–High‐Resolution Mass Spectrometry (GC‐HRMS) and Liquid Chromatography–High‐Resolution Tandem Mass Spectrometry (LC‐HRMS/MS) Open
Cannabis sativa L . constituents, such as cannabinoids, terpenes, flavonoids, and other secondary metabolites, determine the plant's (medicinal) effects and properties in a complex interplay, a phenomenon known as the entourage effect. How…
View article: Evaluation of Bladder Wash as an Alternative Specimen for Postmortem Toxicology: Comparison to Screening Results of Urine and Kidney Tissue
Evaluation of Bladder Wash as an Alternative Specimen for Postmortem Toxicology: Comparison to Screening Results of Urine and Kidney Tissue Open
Urine specimens represent the gold standard for screening analyses in forensic toxicology, but they are not always available postmortem. In this case, alternative specimens must be considered. The current study aimed to systematically eval…
View article: Multianalyte Approach—Including Automated Preparation of Calibrators—for Validated Quantification of 82 Drugs in Whole Blood by Liquid Chromatography–Tandem Mass Spectrometry
Multianalyte Approach—Including Automated Preparation of Calibrators—for Validated Quantification of 82 Drugs in Whole Blood by Liquid Chromatography–Tandem Mass Spectrometry Open
Bioanalysis, such as the quantification of drugs in different matrices, is of great importance in forensic toxicology. Nowadays, mainly so‐called multianalyte approaches are used given their increased speed and effectiveness. However, such…
View article: Comprehensive evaluation of cocaine and its hydroxy metabolites in seized cocaine and a large cohort of hair samples
Comprehensive evaluation of cocaine and its hydroxy metabolites in seized cocaine and a large cohort of hair samples Open
As cocaine (COC) is not only incorporated into hair via blood following ingestion but also by external contamination, hair samples are commonly tested for COC metabolites to prove ingestion. However, COC metabolites can also be present as …
View article: Delirium in Neurocritical Care: Uncovering Undisclosed Psychotropic Substance and Medication Use and Stress Exposure by Hair Analysis
Delirium in Neurocritical Care: Uncovering Undisclosed Psychotropic Substance and Medication Use and Stress Exposure by Hair Analysis Open
Objective In intensive care, delirium is frequent, prolongs the stay, increases health care costs, and worsens patient outcome. Several substances and medications as well as stress can impact the risk of delirium; however, assessment of pr…
View article: Overcoming the clinical challenges of traditional ayahuasca: a first-in-human trial exploring novel routes of administration of N,N-Dimethyltryptamine and harmine
Overcoming the clinical challenges of traditional ayahuasca: a first-in-human trial exploring novel routes of administration of N,N-Dimethyltryptamine and harmine Open
Recently, the Amazonian plant medicine “ayahuasca”—containing the psychedelic compound N,N-dimethyltryptamine (DMT) and numerous β-carboline alkaloids, such as harmine—has been suggested to exhibit beneficial effects in patients with affec…
View article: Untargeted Metabolomics Profiling for Determination of the Time since Deposition of Biofluids in a Forensic Context: A Proof-of-Concept for Urine, Saliva, and Semen in Addition to Blood
Untargeted Metabolomics Profiling for Determination of the Time since Deposition of Biofluids in a Forensic Context: A Proof-of-Concept for Urine, Saliva, and Semen in Addition to Blood Open
In a criminal trial, the reconstruction of a crime is one of the fundamental steps of the prosecution process. Common questions, such as what happened, where and how it happened, and who made it happen, need to be solved. Biological eviden…
View article: Factors influencing the successful implementation of a novel digital health application to streamline multidisciplinary communication across multiple organisations for emergency care
Factors influencing the successful implementation of a novel digital health application to streamline multidisciplinary communication across multiple organisations for emergency care Open
Rationale Delivering optimal patient health care requires interdisciplinary clinician communication. A single communication tool across multiple pre‐hospital and hospital settings, and between hospital departments is a novel solution to cu…
View article: Determination of the Time since Deposition of blood‐traces in a forensic context: Application of untargeted LC‐HR‐MS/MS metabolomics profiling
Determination of the Time since Deposition of blood‐traces in a forensic context: Application of untargeted LC‐HR‐MS/MS metabolomics profiling Open
Being able to attest when a bloodstain was deposited at a crime scene can be invaluable to a prosecution process, and methods to provide that information have long been desired. Determining the Time since Deposition (TsD) of a trace would …
View article: Safety and Efficacy of Tenecteplase and Alteplase in Patients With Tandem Lesion Stroke
Safety and Efficacy of Tenecteplase and Alteplase in Patients With Tandem Lesion Stroke Open
This study provides Class III evidence that TNK is associated with similar rates of intracranial reperfusion, functional outcome, mortality, and sICH compared with alteplase in patients with acute stroke due to TLs. However, the CIs do not…
View article: Nocturnal sodium oxybate increases the anterior cingulate cortex magnetic resonance glutamate signal upon awakening
Nocturnal sodium oxybate increases the anterior cingulate cortex magnetic resonance glutamate signal upon awakening Open
Summary Clinical guidelines recommend sodium oxybate (SXB; the sodium salt of γ‐hydroxybutyrate) for the treatment of disturbed sleep and excessive daytime sleepiness in narcolepsy, yet the underlying mode of action is elusive. In a random…
View article: Do dried blood spots have the potential to support result management processes in routine sports drug testing?—Part 3: LC–MS/MS‐based peptide analysis for dried blood spot sampling time point estimation
Do dried blood spots have the potential to support result management processes in routine sports drug testing?—Part 3: LC–MS/MS‐based peptide analysis for dried blood spot sampling time point estimation Open
Along with the recent acknowledgement of the World Anti‐Doping Agency to use dried blood spot (DBS) samples for routine doping control purposes, there have been propositions to use DBS as a matrix that allows regular proactive remotely sup…
View article: Inhaled mosliciguat (BAY 1237592): targeting pulmonary vasculature via activating apo-sGC
Inhaled mosliciguat (BAY 1237592): targeting pulmonary vasculature via activating apo-sGC Open
Background Oxidative stress associated with severe cardiopulmonary diseases leads to impairment in the nitric oxide/soluble guanylate cyclase signaling pathway, shifting native soluble guanylate cyclase toward heme-free apo-soluble guanyla…
View article: Determination of the Time since Deposition of Blood Traces Utilizing a Liquid Chromatography–Mass Spectrometry-Based Proteomics Approach
Determination of the Time since Deposition of Blood Traces Utilizing a Liquid Chromatography–Mass Spectrometry-Based Proteomics Approach Open
Knowledge about when a bloodstain was deposited at a crime scene can be of critical value in forensic investigation. A donor of a genetically identified bloodstain could be linked to a suspected time frame and the crime scene itself. Deter…
View article: Real-world, feasibility study to investigate the use of a multidisciplinary app (Pulsara) to improve prehospital communication and timelines for acute stroke/STEMI care
Real-world, feasibility study to investigate the use of a multidisciplinary app (Pulsara) to improve prehospital communication and timelines for acute stroke/STEMI care Open
Objectives To determine if a digital communication app improves care timelines for patients with suspected acute stroke/ST-elevation myocardial infarction (STEMI). Design Real-world feasibility study, quasi-experimental design. Setting Pre…
View article: Towards a New Qualitative Screening Assay for Synthetic Cannabinoids Using Metabolomics and Machine Learning
Towards a New Qualitative Screening Assay for Synthetic Cannabinoids Using Metabolomics and Machine Learning Open
Background Synthetic cannabinoids (SCs) are steadily emerging on the drug market. To remain competitive in clinical or forensic toxicology, new screening strategies including high-resolution mass spectrometry (HRMS) are required. Machine l…