Thomas Laurell
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View article: Impact of staining on cell acoustic properties
Impact of staining on cell acoustic properties Open
Cell staining techniques are essential for cell visualization and bioanalysis, providing valuable insights into cellular structure, function, and behavior. Despite their significance, the impact of staining processes, such as dye penetrati…
View article: Accessing the proteome of extracellular vesicles via rapid acoustic isolation of a minute human blood plasma sample
Accessing the proteome of extracellular vesicles via rapid acoustic isolation of a minute human blood plasma sample Open
Our microfluidics-based approach to EV isolation enables rapid processing of an individual minute blood plasma sample, demonstrating that personal proteomic information associated with EVs can be detected when acoustic trapping is used as …
View article: Proteomic Profiling of Acoustically Isolated Extracellular Vesicles from Blood Plasma during Murine Bacterial Sepsis
Proteomic Profiling of Acoustically Isolated Extracellular Vesicles from Blood Plasma during Murine Bacterial Sepsis Open
Sepsis is a life-threatening condition caused by a dysregulated host response to an infection and is a leading cause of death worldwide. The condition is variable, which in combination with insufficient clinical markers, makes it challengi…
View article: Acoustophoresis-based blood sampling and plasma separation for potentially minimizing sampling-related blood loss
Acoustophoresis-based blood sampling and plasma separation for potentially minimizing sampling-related blood loss Open
Objectives Frequent blood sampling in vulnerable patient groups, such as prematurely born infants, can lead to significant blood loss and increased transfusion needs. Current pre-analytical technology requires comparably large blood volume…
View article: Acoustofluidic Chromatography for Extracellular Vesicle Enrichment from 4 μL Blood Plasma Samples
Acoustofluidic Chromatography for Extracellular Vesicle Enrichment from 4 μL Blood Plasma Samples Open
We present a novel acoustofluidic chromatography platform for high-throughput nanoparticle trapping and enrichment, with a focus on extracellular vesicles (EVs) from blood plasma. The system features a packed bed of polystyrene beads insid…
View article: Optimizing the quality of acoustophoretic separation by the in-flow mobility-ratio method
Optimizing the quality of acoustophoretic separation by the in-flow mobility-ratio method Open
Achieving good acoustic particle or cell separation performance requires skilled operators, who must carefully fine-tune the input parameters (actuation voltages, flow rates, and flow split ratios). Often the fine-tuning is done by time-co…
View article: Whole-channel acoustic energy and acoustophoretic efficiency frequency spectrum by the in-flow focusing method
Whole-channel acoustic energy and acoustophoretic efficiency frequency spectrum by the in-flow focusing method Open
The in-flow focusing method, introduced herein, enables rapid and straightforward measurements of the whole-channel acoustic energy of acoustic flow-through devices. The method is applied to assess the whole-channel acoustic energy and aco…
View article: Acoustic Enrichment of Heterogeneous Circulating Tumor Cells and Clusters from Metastatic Prostate Cancer Patients
Acoustic Enrichment of Heterogeneous Circulating Tumor Cells and Clusters from Metastatic Prostate Cancer Patients Open
Our preliminary data indicate that acoustophoresis provides excellent possibilities to detect and characterize CTC clusters as a putative marker of metastatic disease and outcomes. Moreover, acoustophoresis enables the sensitive label-free…
View article: Silica seed particles improve the efficiency and throughput of nanoparticle acoustic trapping
Silica seed particles improve the efficiency and throughput of nanoparticle acoustic trapping Open
Silica has rarely been used as a seed particle material in acoustic trapping of nanoparticles. Here we use fluorescent nanoparticles, which are frequently used as a model system, to demonstrate that throughput and nanoparticle trapping eff…
View article: Identifying secreted biomarkers of dopaminergic ventral midbrain progenitor cells
Identifying secreted biomarkers of dopaminergic ventral midbrain progenitor cells Open
Background Ventral midbrain (VM) dopaminergic progenitor cells derived from human pluripotent stem cells have the potential to replace endogenously lost dopamine neurons and are currently in preclinical and clinical development for treatme…
View article: Acoustic enrichment of heterogenous circulating tumor cells and clusters from patients with metastatic prostate cancer
Acoustic enrichment of heterogenous circulating tumor cells and clusters from patients with metastatic prostate cancer Open
Background There are important unmet clinical needs to develop cell enrichment technologies to enable unbiased label-free isolation of both single cell and clusters of circulating tumor cells (CTCs) manifesting heterogeneous lineage specif…
View article: Displacement of PAMAM-Au via acoustic streaming on an electrochemical immunosensing platform
Displacement of PAMAM-Au via acoustic streaming on an electrochemical immunosensing platform Open
The displacement of an electroactive monitoring agent, i.e., polyamidoamine dendrimers encapsulated gold nanoparticles (PAMAM-Au) upon the presence of a target antibody via acoustic streaming has been studied. Acoustic streaming has been u…
View article: Ultrasound standing wave spatial patterning of human umbilical vein endothelial cells for 3D micro-vascular networks formation
Ultrasound standing wave spatial patterning of human umbilical vein endothelial cells for 3D micro-vascular networks formation Open
Generating functional and perfusable micro-vascular networks is an important goal for the fabrication of large and three-dimensional tissues. Up to now, the fabrication of micro-vascular networks is a complicated multitask involving severa…
View article: Acoustofluidic Properties of Polystyrene Microparticles
Acoustofluidic Properties of Polystyrene Microparticles Open
Acoustophoresis has become a powerful tool to separate microparticles and cells, based on their material and biophysical properties, and is gaining popularity in clinical and biomedical research. One major application of acoustophoresis is…
View article: Identifying secreted biomarkers of dopaminergic ventral midbrain progenitor cells
Identifying secreted biomarkers of dopaminergic ventral midbrain progenitor cells Open
Background:Ventral midbrain (VM) dopaminergic progenitor cells derived from human pluripotent stem cells have the potential to replace endogenously lost dopamine neurons and are currently in preclinical and clinical development for treatme…
View article: Extracellular Vesicle-Associated TWEAK Contributes to Vascular Inflammation and Remodeling During Acute Cellular Rejection
Extracellular Vesicle-Associated TWEAK Contributes to Vascular Inflammation and Remodeling During Acute Cellular Rejection Open
Acute cellular rejection (ACR) is a leading cause of graft loss and death after heart transplantation despite effective immunosuppressive therapies. The identification of factors that impair graft vascular barrier function or promote immun…
View article: Advancement and obstacles in microfluidics-based isolation of extracellular vesicles
Advancement and obstacles in microfluidics-based isolation of extracellular vesicles Open
There is a great need for techniques which enable reproducible separation of extracellular vesicles (EVs) from biofluids with high recovery, purity and throughput. The development of new techniques for isolation of EVs from minute sample v…
View article: Gap Distance Between Pearl Chains in Acoustic Manipulation
Gap Distance Between Pearl Chains in Acoustic Manipulation Open
We present a theory to compute the stable gap (interparticle) distance between particle chains collected in the pressure node of an acoustic standing wave. The primary and secondary acoustic radiation forces are the two competing forces th…
View article: Enhancement of Acoustic Energy Density in Bulk-Wave-Acoustophoresis Devices Using Side Actuation
Enhancement of Acoustic Energy Density in Bulk-Wave-Acoustophoresis Devices Using Side Actuation Open
A high acoustic energy density is required in the acoustic resonator to increase the throughput of acoustophoresis devices. In this study, through both experiments and numerical simulations, we find that the energy density in bulk-wave-aco…
View article: Inertia-Induced Breakdown of Acoustic Sorting Efficiency at High Flow Rates
Inertia-Induced Breakdown of Acoustic Sorting Efficiency at High Flow Rates Open
The clinical utility of microfluidic techniques is often hampered by an unsatisfying sample throughput. Here, the effect of inertial forces on acoustofluidic particle sorting at high sample throughputs is investigated experimentally and th…
View article: MSLibrarian: Optimized Predicted Spectral Libraries for Data-Independent Acquisition Proteomics
MSLibrarian: Optimized Predicted Spectral Libraries for Data-Independent Acquisition Proteomics Open
Data-independent acquisition-mass spectrometry (DIA-MS) is the method of choice for deep, consistent, and accurate single-shot profiling in bottom-up proteomics. While classic workflows for targeted quantification from DIA-MS data require …
View article: Two-Step Acoustophoresis Separation of Live Tumor Cells from Whole Blood
Two-Step Acoustophoresis Separation of Live Tumor Cells from Whole Blood Open
There is an unmet clinical need to extract living circulating tumor cells (CTCs) for functional studies and in vitro expansion to enable drug testing and predict responses to therapy in metastatic cancer. Here, we present a novel two-step …
View article: MSLibrarian: Spectral Warehouse DBs for some common species
MSLibrarian: Spectral Warehouse DBs for some common species Open
Spectral Warehouse DBs for some common species to be used with the R software package MSLibrarian. More information on how to use the Spectral Warehouse DBs, and how to use MSLibrarian, can be found at: https://github.com/MarcIsak/MSLibrar…
View article: MSLibrarian: Spectral Warehouse DBs for some common species
MSLibrarian: Spectral Warehouse DBs for some common species Open
Spectral Warehouse DBs for some common species to be used with the R software package MSLibrarian. More information on how to use the Spectral Warehouse DBs, and how to use MSLibrarian, can be found at: https://github.com/MarcIsak/MSLibrar…