Thomas N. Wight
YOU?
Author Swipe
View article: Hyaluronan Accumulates in Inflamed Lymph Nodes and Promotes B‐Cell Activation
Hyaluronan Accumulates in Inflamed Lymph Nodes and Promotes B‐Cell Activation Open
The extracellular matrix component hyaluronan (HA) plays important roles in inflammation and immune regulation. However, its involvement in lymphoid tissue during autoreactive processes remains poorly understood. Here, using the DORmO mous…
View article: Hymecromone Promotes Longevity and Insulin Sensitivity in Mice
Hymecromone Promotes Longevity and Insulin Sensitivity in Mice Open
Given that the extracellular matrix polymer hyaluronan (HA) has been implicated in longevity, we asked whether 4-methylumbelliferone (4-MU), an inhibitor of HA synthesis, impacts lifespan in mice. We designed a prospective study of long-te…
View article: Response to retention hypothesis as a source of targets for arterial wall-directed therapies to prevent atherosclerosis: A critical review
Response to retention hypothesis as a source of targets for arterial wall-directed therapies to prevent atherosclerosis: A critical review Open
View article: Supplementary Figure 7 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 7 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 7 shows representative images of each phenotype
View article: Supplementary Figure 3 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 3 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 3 describes the transwell model
View article: FIGURE 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
VCAN is associated with T-cell localization. A, Matrix index from Pearce and colleagues (14). Arrows indicate upregulation or downregulation at gene (G) or protein (P) level. B, Level of gene expression of top five upregulate…
View article: Data from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Data from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Immunotherapies for cancers of epithelial origin have limited efficacy, and a growing body of evidence links the composition of extracellular matrix (ECM) with the likelihood of a favorable response to treatment. The ECM may be considered …
View article: Supplementary Figure 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 1 shows how VCAN was enriched and mass spectrometry analysis of the sample
View article: FIGURE 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Determining the immune phenotype of tumor tissues. A total of 26 TNBC tissues were analyzed for the TIP. A, Annotation of slide on QuPath for analysis of the four regions: Stroma, Outer Invasive, Inner Invasive, and Tumor Core. Numb…
View article: Supplementary Figure 5 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 5 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 5 outlines the analysis of matrix proteins in TMAs.
View article: TABLE 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
TABLE 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Primers for qRT-PCR. Primer sequences for VCAN isoforms and housekeeping gene RPS13
View article: FIGURE 4 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 4 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Expression of VCAN in tissues and cells. A, VCAN protein expression from IHC compared with gene expression from RNAscope. Spearman rank correlation. B, Comparison of FAP+ cells and percentage of α-SMA staining with…
View article: Supplementary Figure 5 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 5 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 5 outlines the analysis of matrix proteins in TMAs.
View article: TABLE 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
TABLE 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Antibodies for IHC. Optimized parameters for each antibody
View article: FIGURE 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Determining the immune phenotype of tumor tissues. A total of 26 TNBC tissues were analyzed for the TIP. A, Annotation of slide on QuPath for analysis of the four regions: Stroma, Outer Invasive, Inner Invasive, and Tumor Core. Numb…
View article: Supplementary Figure 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 2 shows CS analysis from FFPE tissue sections
View article: Supplementary Figure 4 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 4 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 4 shows different method of TIP analysis and the TIPs from different subsets of tissues.
View article: TABLE 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
TABLE 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Antibodies for IHC. Optimized parameters for each antibody
View article: FIGURE 6 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 6 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
CS isomers expression in tissues. A, Structure of CS isoforms following digestion of the chains with ChABC. CS extracted from FFPE tissues in the epithelial zone and stromal zone. CS isoforms analyzed by MS to identify proportion of…
View article: FIGURE 7 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 7 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Effect of CS toward T-cell invasion. A, CS MS completed on the crude and enriched protein from HMF3S and HCC38 cell lines. CS isomers and A/C ratio determined. B, Transwell assay completed with 5 µg of each protein sample. Ea…
View article: FIGURE 6 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 6 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
CS isomers expression in tissues. A, Structure of CS isoforms following digestion of the chains with ChABC. CS extracted from FFPE tissues in the epithelial zone and stromal zone. CS isoforms analyzed by MS to identify proportion of…
View article: Supplementary Figure 6 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 6 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 6 shows VCAN analysis in the TIPs from the second tissue subset
View article: FIGURE 4 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 4 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Expression of VCAN in tissues and cells. A, VCAN protein expression from IHC compared with gene expression from RNAscope. Spearman rank correlation. B, Comparison of FAP+ cells and percentage of α-SMA staining with…
View article: FIGURE 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
VCAN is associated with T-cell localization. A, Matrix index from Pearce and colleagues (14). Arrows indicate upregulation or downregulation at gene (G) or protein (P) level. B, Level of gene expression of top five upregulate…
View article: Supplementary Figure 8 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 8 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 8 summarises the results identified.
View article: TABLE 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
TABLE 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Primers for qRT-PCR. Primer sequences for VCAN isoforms and housekeeping gene RPS13
View article: Supplementary Figure 4 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 4 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 4 shows different method of TIP analysis and the TIPs from different subsets of tissues.
View article: Supplementary Figure 7 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 7 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 7 shows representative images of each phenotype
View article: Supplementary Figure 8 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 8 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 8 summarises the results identified.
View article: FIGURE 7 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 7 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Effect of CS toward T-cell invasion. A, CS MS completed on the crude and enriched protein from HMF3S and HCC38 cell lines. CS isomers and A/C ratio determined. B, Transwell assay completed with 5 µg of each protein sample. Ea…