Thomas Nebl
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View article: Flp/ <i>FRT</i> -mediated disruption of <i>ptex150</i> and <i>exp2</i> in <i>Plasmodium falciparum</i> sporozoites inhibits liver-stage development
Flp/ <i>FRT</i> -mediated disruption of <i>ptex150</i> and <i>exp2</i> in <i>Plasmodium falciparum</i> sporozoites inhibits liver-stage development Open
Plasmodium falciparum causes severe malaria and assembles a protein translocon (PTEX) complex at the parasitophorous vacuole membrane (PVM) of infected erythrocytes, through which several hundred proteins are exported to facilitate growth.…
View article: Multiplexed Native Mass Spectrometry Determination of Ligand Selectivity for Fatty Acid-Binding Proteins
Multiplexed Native Mass Spectrometry Determination of Ligand Selectivity for Fatty Acid-Binding Proteins Open
Although multiple approaches for characterizing protein-ligand interactions are available in target-based drug discovery, their throughput for determining selectivity is quite limited. Herein, we describe the application of native mass spe…
View article: Characterization of novel mevalonate kinases from the tardigrade <i>Ramazzottius varieornatus</i> and the psychrophilic archaeon <i>Methanococcoides burtonii</i>
Characterization of novel mevalonate kinases from the tardigrade <i>Ramazzottius varieornatus</i> and the psychrophilic archaeon <i>Methanococcoides burtonii</i> Open
Mevalonate kinase is central to the isoprenoid biosynthesis pathway. Here, high-resolution X-ray crystal structures of two mevalonate kinases are presented: a eukaryotic protein from Ramazzottius varieornatus and an archaeal protein from M…
View article: Effect of composition, casein genetic variants and glycosylation degree on bovine milk whipping properties
Effect of composition, casein genetic variants and glycosylation degree on bovine milk whipping properties Open
This study investigated the individual and combined effects of ĸ-Casein (ĸ-CN; AA, AB, BB), β-Casein (β-CN; A1A1, A1A2, A2A2) and high and low ratios of glycosylated ĸ-CN to total ĸ-CN, referred to as the glycosylation degree (GD), on bovi…
View article: High ploidy large cytoplasmic megakaryocytes are hematopoietic stem cells regulators and essential for platelet production
High ploidy large cytoplasmic megakaryocytes are hematopoietic stem cells regulators and essential for platelet production Open
Megakaryocytes (MK) generate platelets. Recently, we and others, have reported MK also regulate hematopoietic stem cells (HSC). Here we show high ploidy large cytoplasmic megakaryocytes (LCM) are critical negative regulators of HSC and cri…
View article: Plasmodium falciparum exoerythrocytic forms require the PTEX translocon for development in human hepatocytes
Plasmodium falciparum exoerythrocytic forms require the PTEX translocon for development in human hepatocytes Open
Plasmodium falciparum assembles a protein translocon (PTEX) at the parasitophorous vacuole membrane (PVM) of infected erythrocytes, through which several hundred proteins are exported. The preceding Plasmodium liver stage develops in hepat…
View article: The role of glycosylation in amyloid fibril formation of bovine κ-casein
The role of glycosylation in amyloid fibril formation of bovine κ-casein Open
View article: Design of a methotrexate-controlled chemical dimerization system and its use in bio-electronic devices
Design of a methotrexate-controlled chemical dimerization system and its use in bio-electronic devices Open
Natural evolution produced polypeptides that selectively recognize chemical entities and their polymers, ranging from ions to proteins and nucleic acids. Such selective interactions serve as entry points to biological signaling and metabol…
View article: Purification of an insect juvenile hormone receptor complex enables insights into its post-translational phosphorylation
Purification of an insect juvenile hormone receptor complex enables insights into its post-translational phosphorylation Open
Juvenile hormone (JH) plays vital roles in insect reproduction, development, and in many aspects of physiology. JH primarily acts at the gene-regulatory level through interaction with an intracellular receptor (JH receptor [JHR]), a ligand…
View article: The Glitazone Class of Drugs as Carbonic Anhydrase Inhibitors—A Spin-Off Discovery from Fragment Screening
The Glitazone Class of Drugs as Carbonic Anhydrase Inhibitors—A Spin-Off Discovery from Fragment Screening Open
The approved drugs that target carbonic anhydrases (CA, EC 4.2.1.1), a family of zinc metalloenzymes, comprise almost exclusively of primary sulfonamides (R-SO2NH2) as the zinc binding chemotype. New clinical applications for CA inhibitors…
View article: 20S proteasomes secreted by the malaria parasite promote its growth
20S proteasomes secreted by the malaria parasite promote its growth Open
Mature red blood cells (RBCs) lack internal organelles and canonical defense mechanisms, making them both a fascinating host cell, in general, and an intriguing choice for the deadly malaria parasite Plasmodium falciparum ( Pf ), in partic…
View article: Podocalyxin is a key negative regulator of human endometrial epithelial receptivity for embryo implantation
Podocalyxin is a key negative regulator of human endometrial epithelial receptivity for embryo implantation Open
STUDY QUESTION How is endometrial epithelial receptivity, particularly adhesiveness, regulated at the luminal epithelial surface for embryo implantation in the human? SUMMARY ANSWER Podocalyxin (PCX), a transmembrane protein, was identifie…
View article: The X-ray crystal structure of the N-terminal domain of Ssr4, a <i>Schizosaccharomyces pombe</i> chromatin-remodelling protein
The X-ray crystal structure of the N-terminal domain of Ssr4, a <i>Schizosaccharomyces pombe</i> chromatin-remodelling protein Open
Ssr4 is a yeast protein from Schizosaccharomyces pombe and is an essential part of the chromatin-remodelling [SWI/SNF and RSC (remodelling the structure of chromatin)] complexes found in S. pombe . These complexes (or their homologues) reg…
View article: Crystal structures of human ENPP1 in apo and bound forms
Crystal structures of human ENPP1 in apo and bound forms Open
Cancer is one of the leading causes of mortality in humans, and recent work has focused on the area of immuno-oncology, in which the immune system is used to specifically target cancerous cells. Ectonucleotide pyrophosphatase/phosphodieste…
View article: The evolving story of AtzT, a periplasmic binding protein
The evolving story of AtzT, a periplasmic binding protein Open
Atrazine is an s -triazine-based herbicide that is used in many countries around the world in many millions of tons per year. A small number of organisms, such as Pseudomonas sp. strain ADP, have evolved to use this modified s -triazine as…
View article: Structures of the transcriptional regulator BgaR, a lactose sensor
Structures of the transcriptional regulator BgaR, a lactose sensor Open
The structure of BgaR, a transcriptional regulator of the lactose operon in Clostridium perfringens , has been solved by SAD phasing using a mercury derivative. BgaR is an exquisite sensor of lactose, with a binding affinity in the low-mic…
View article: Engineered Enzymes that Retain and Regenerate their Cofactors Enable Continuous-Flow Biocatalysis
Engineered Enzymes that Retain and Regenerate their Cofactors Enable Continuous-Flow Biocatalysis Open
Biocatalysis is used for many chemical syntheses due to its high catalytic rates, specificities and operation under ambient conditions [1][1], [2][2]. Continuous-flow chemistry offers advantages to biocatalysis, avoiding process issues cau…
View article: Gastro-intestinal Digestibility of Processed Forms of Cow’s Milk Proteins under Simulated Infant and Adult Conditions Characterised by in vitro Methods
Gastro-intestinal Digestibility of Processed Forms of Cow’s Milk Proteins under Simulated Infant and Adult Conditions Characterised by in vitro Methods Open
Human digestion involves chemical, biochemical and fermentative processes integrated to achieve optimal nutrient absorption along the gastro-intestinal (GI) tract. In particular, efficient gastro-intestinal enzymatic digestion of proteins …
View article: The malaria <scp>PTEX</scp> component <scp>PTEX</scp>88 interacts most closely with <scp>HSP</scp>101 at the host–parasite interface
The malaria <span>PTEX</span> component <span>PTEX</span>88 interacts most closely with <span>HSP</span>101 at the host–parasite interface Open
The pathogenic nature of malaria infections is due in part to the export of hundreds of effector proteins that actively remodel the host erythrocyte. The Plasmodium translocon of exported proteins ( PTEX ) has been shown to facilitate the …
View article: An unexpected vestigial protein complex reveals the evolutionary origins of an s-triazine catabolic enzyme
An unexpected vestigial protein complex reveals the evolutionary origins of an s-triazine catabolic enzyme Open
View article: The exported chaperone Hsp70-x supports virulence functions for Plasmodium falciparum blood stage parasites
The exported chaperone Hsp70-x supports virulence functions for Plasmodium falciparum blood stage parasites Open
Malaria is caused by five different Plasmodium spp. in humans each of which modifies the host erythrocyte to survive and replicate. The two main causes of malaria, P. falciparum and P. vivax, differ in their ability to cause severe disease…
View article: Plasmodium falciparum ligand binding to erythrocytes induce alterations in deformability essential for invasion
Plasmodium falciparum ligand binding to erythrocytes induce alterations in deformability essential for invasion Open
The most lethal form of malaria in humans is caused by Plasmodium falciparum. These parasites invade erythrocytes, a complex process involving multiple ligand-receptor interactions. The parasite makes initial contact with the erythrocyte f…
View article: Author response: Plasmodium falciparum ligand binding to erythrocytes induce alterations in deformability essential for invasion
Author response: Plasmodium falciparum ligand binding to erythrocytes induce alterations in deformability essential for invasion Open
View article: Essential Role of the PfRh5/PfRipr/CyRPA Complex during Plasmodium falciparum Invasion of Erythrocytes
Essential Role of the PfRh5/PfRipr/CyRPA Complex during Plasmodium falciparum Invasion of Erythrocytes Open
View article: Proteomic analysis reveals novel proteins associated with the<i>Plasmodium</i>protein exporter PTEX and a loss of complex stability upon truncation of the core PTEX component, PTEX150
Proteomic analysis reveals novel proteins associated with the<i>Plasmodium</i>protein exporter PTEX and a loss of complex stability upon truncation of the core PTEX component, PTEX150 Open
The Plasmodium translocon for exported proteins (PTEX) has been established as the machinery responsible for the translocation of all classes of exported proteins beyond the parasitophorous vacuolar membrane of the intraerythrocytic malari…
View article: Export of malaria proteins requires co-translational processing of the PEXEL motif independent of phosphatidylinositol-3-phosphate binding
Export of malaria proteins requires co-translational processing of the PEXEL motif independent of phosphatidylinositol-3-phosphate binding Open