Thomas S. Hnasko
YOU?
Author Swipe
View article: GABA-glutamate corelease is a mechanism for state-dependent neurotransmission
GABA-glutamate corelease is a mechanism for state-dependent neurotransmission Open
Summary Ventral tegmental area neurons projecting to lateral habenula (LHb) corelease the main inhibitory and excitatory transmitters, GABA and glutamate (VTA GG ). Yet the functional role of this synchronous signal remains unclear. We hyp…
View article: v2 RNAscope v1
v2 RNAscope v1 Open
This is a protocol for performing RNAscope® in situ hybridization analysis on fixed-frozen mouse brain tissue using the RNAscope® Multiplex Fluorescent v2 kit (Advanced Cell Diagnostics; ACD). It is similar to the v2 protocol provided by A…
View article: Mu-opioid receptor activation potentiates excitatory transmission at the habenulo-peduncular synapse
Mu-opioid receptor activation potentiates excitatory transmission at the habenulo-peduncular synapse Open
Identifying how opioids modulate signaling in relevant neurocircuitry is essential for developing new therapeutic strategies for opioid addiction. The medial habenula (MHb) is a mu-opioid receptor (MOR) hotspot that projects predominantly …
View article: Parkinson’s Disease-vulnerable and -resilient dopamine neurons display opposite responses to excitatory input
Parkinson’s Disease-vulnerable and -resilient dopamine neurons display opposite responses to excitatory input Open
Dopamine (DA) neurons of the substantia nigra (SN) are essential for motor control and selectively degenerate in Parkinson’s disease (PD). However, DA neurons are molecularly heterogeneous, with some showing greater vulnerability and other…
View article: Ventral tegmental area interneurons revisited: GABA and glutamate projection neurons make local synapses
Ventral tegmental area interneurons revisited: GABA and glutamate projection neurons make local synapses Open
The ventral tegmental area (VTA) contains projection neurons that release the neurotransmitters dopamine, GABA, and/or glutamate from distal synapses. VTA also contains GABA neurons that synapse locally on to dopamine neurons, synapses wid…
View article: Evidence for low affinity of GABA at the vesicular monoamine transporter VMAT2 – Implications for transmitter co-release from dopamine neurons
Evidence for low affinity of GABA at the vesicular monoamine transporter VMAT2 – Implications for transmitter co-release from dopamine neurons Open
Midbrain dopamine (DA) neurons comprise a heterogeneous population of cells. For instance, some DA neurons express the vesicular glutamate transporter VGLUT2 allowing these cells to co-release DA and glutamate. Additionally, GABA may be co…
View article: Ventral tegmental area interneurons revisited: GABA and glutamate projection neurons make local synapses
Ventral tegmental area interneurons revisited: GABA and glutamate projection neurons make local synapses Open
The ventral tegmental area (VTA) contains projection neurons that release the neurotransmitters dopamine, GABA, and/or glutamate from distal synapses. VTA also contains GABA neurons that synapse locally on to dopamine neurons, synapses wid…
View article: Mu-opioid receptor activation potentiates excitatory transmission at the habenulo-peduncular synapse
Mu-opioid receptor activation potentiates excitatory transmission at the habenulo-peduncular synapse Open
The continuing opioid epidemic poses a huge burden on public health. Identifying the neurocircuitry involved and how opioids modulate their signaling is essential for developing new therapeutic strategies. The medial habenula (MHb) is a sm…
View article: Proportion and distribution of neurotransmitter-defined cell types in the ventral tegmental area and substantia nigra pars compacta
Proportion and distribution of neurotransmitter-defined cell types in the ventral tegmental area and substantia nigra pars compacta Open
Most studies on the ventral tegmental area (VTA) and substantia nigra pars compacta (SNc) have focused on dopamine neurons and their role in processes such as motivation, learning, movement, and associated disorders such as addiction and P…
View article: ZSGreen x TH Colocalization Analysis v1
ZSGreen x TH Colocalization Analysis v1 Open
Colocalization analysis of TH and ZSGreen using immunohistochemistry
View article: Immunohistochemistry for anti-TH v1
Immunohistochemistry for anti-TH v1 Open
Hnasko lab protocol for anti-TH immunohistochemistry
View article: Viral overexpression of human alpha-synuclein in mouse substantia nigra dopamine neurons results in hyperdopaminergia but no neurodegeneration
Viral overexpression of human alpha-synuclein in mouse substantia nigra dopamine neurons results in hyperdopaminergia but no neurodegeneration Open
Loss of select neuronal populations such as midbrain dopamine (DA) neurons is a pathological hallmark of Parkinson's disease (PD). The small neuronal protein α-synuclein has been related both genetically and neuropathologically to PD, yet …
View article: Ventral tegmental area interneurons revisited: GABA and glutamate projection neurons make local synapses
Ventral tegmental area interneurons revisited: GABA and glutamate projection neurons make local synapses Open
The ventral tegmental area (VTA) contains projection neurons that release the neurotransmitters dopamine, GABA, and/or glutamate from distal synapses. VTA also contains GABA neurons that synapse locally on to VTA dopamine neurons, synapses…
View article: Ventral tegmental area interneurons revisited: GABA and glutamate projection neurons make local synapses
Ventral tegmental area interneurons revisited: GABA and glutamate projection neurons make local synapses Open
The ventral tegmental area (VTA) contains projection neurons that release the neurotransmitters dopamine, GABA, and/or glutamate from distal synapses. VTA also contains GABA neurons that synapse locally on to dopamine neurons, synapses wid…
View article: Intra-cardiac mouse perfusion v1
Intra-cardiac mouse perfusion v1 Open
Hnasko lab intra-cardiac mouse perfusion protocol
View article: Cryoprotection of mouse brain tissue v1
Cryoprotection of mouse brain tissue v1 Open
Fixed brains are submerged in 30% sucrose to cryoprotect prior to freezing and cutting cryosections.
View article: Coronal cryosectioning of mouse brains v1
Coronal cryosectioning of mouse brains v1 Open
Cryostat coronal cryosectioning of mouse brain tissue
View article: Immunohistochemistry for anti-GFP v1
Immunohistochemistry for anti-GFP v1 Open
Hnasko lab protocol for anti-GFP immunohistochemistry
View article: Ventral tegmental area interneurons revisited: GABA and glutamate projection neurons make local synapses
Ventral tegmental area interneurons revisited: GABA and glutamate projection neurons make local synapses Open
The ventral tegmental area (VTA) contains projection neurons that release the neurotransmitters dopamine, GABA, and/or glutamate from distal synapses. VTA also contains GABA neurons that synapse locally on to dopamine neurons, synapses wid…
View article: Viral overexpression of human alpha-synuclein in mouse substantia nigra dopamine neurons results in hyperdopaminergia but no neurodegeneration
Viral overexpression of human alpha-synuclein in mouse substantia nigra dopamine neurons results in hyperdopaminergia but no neurodegeneration Open
Loss of select neuronal populations such as midbrain dopamine (DA) neurons is a pathological hallmark of Parkinson’s disease (PD). The small neuronal protein α-synuclein has been related both genetically and neuropathologically to PD, yet …
View article: Proportion and distribution of neurotransmitter-defined cell types in the ventral tegmental area and substantia nigra pars compacta
Proportion and distribution of neurotransmitter-defined cell types in the ventral tegmental area and substantia nigra pars compacta Open
Most studies on the ventral tegmental area (VTA) and substantia nigra pars compacta (SNc) have focused on dopamine neurons and their role in processes such as motivation, learning, movement, and associated disorders such as addiction and P…
View article: v2 RNAscope v1
v2 RNAscope v1 Open
This is a protocol for performing RNAscope® in situ hybridization analysis on fixed-frozen mouse brain tissue using the RNAscope® Multiplex Fluorescent v2 kit (Advanced Cell Diagnostics; ACD). It is similar to the v2 protocol provided by A…
View article: Preparation of Recording ACSF (artificial cerebrospinal fluid) for slice electrophysiology v1
Preparation of Recording ACSF (artificial cerebrospinal fluid) for slice electrophysiology v1 Open
Hnasko lab- preparation of Recording ACSF Recording ACSF is used for incubating brain slices during cell-attached or whole-cell electrophysiological recordings of neurons.
View article: Preparation of Holding ACSF (artificial cerebrospinal fluid) for slice electrophysiology v1
Preparation of Holding ACSF (artificial cerebrospinal fluid) for slice electrophysiology v1 Open
Hnasko lab- preparation of Holding ACSF Holding ACSF is used for incubating brain slices prior to cell-attached or whole-cell electrophysiological recordings of neurons.