Thomas Scheper
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View article: Influence of species composition and cultivation condition on peri-implant biofilm dysbiosis in vitro
Influence of species composition and cultivation condition on peri-implant biofilm dysbiosis in vitro Open
Introduction Changes in bacterial species composition within oral biofilms, known as biofilm dysbiosis, are associated with the development of severe oral diseases. To better understand this process and help establish early detection syste…
View article: Influence of species composition and cultivation condition on peri-implant biofilm dysbiosis in vitro
Influence of species composition and cultivation condition on peri-implant biofilm dysbiosis in vitro Open
Background Changes in bacterial species composition within oral biofilms, known as biofilm dysbiosis, are associated with the development of severe oral diseases. To better understand this process and help establish early detection systems…
View article: Enhancing chemotherapeutic efficacy: Niosome‐encapsulated Dox‐Cis with <scp>MUC</scp>‐1 aptamer
Enhancing chemotherapeutic efficacy: Niosome‐encapsulated Dox‐Cis with <span>MUC</span>‐1 aptamer Open
Background Cancer remains a formidable global health challenge, currently affecting nearly 20 million individuals worldwide. Due to the absence of universally effective treatments, ongoing research explores diverse strategies to combat thi…
View article: A Novel Artificial Coronary Plaque to Model Coronary Heart Disease
A Novel Artificial Coronary Plaque to Model Coronary Heart Disease Open
Background: Experimental coronary artery interventions are currently being performed on non-diseased blood vessels in healthy animals. To provide a more realistic pathoanatomical scenario for investigations on novel interventional and surg…
View article: Photonic Si microwell architectures for rapid antifungal susceptibility determination of <i>Candida auris</i>
Photonic Si microwell architectures for rapid antifungal susceptibility determination of <i>Candida auris</i> Open
The application of a photonic silicon chip-based optical sensor system for expeditious and phenotypic antifungal susceptibility testing of Candida auris is presented.
View article: Establishment of a Perfusion Process with Antibody-Producing CHO Cells Using a 3D-Printed Microfluidic Spiral Separator with Web-Based Flow Control
Establishment of a Perfusion Process with Antibody-Producing CHO Cells Using a 3D-Printed Microfluidic Spiral Separator with Web-Based Flow Control Open
Monoclonal antibodies are increasingly dominating the market for human therapeutic and diagnostic agents. For this reason, continuous methods—such as perfusion processes—are being explored and optimized in an ongoing effort to increase pro…
View article: Overview Contents: Chemie Ingenieur Technik 4/2023
Overview Contents: Chemie Ingenieur Technik 4/2023 Open
View article: Vorschau: Chem. Ing. Tech. 4/2023
Vorschau: Chem. Ing. Tech. 4/2023 Open
View article: Employing QbD strategies to assess the impact of cell viability and density on the primary recovery of monoclonal antibodies
Employing QbD strategies to assess the impact of cell viability and density on the primary recovery of monoclonal antibodies Open
Quality by Design (QbD) is one of the most important tools for the implementation of Process Analytical Technology (PAT) in biopharmaceutical production. For optimal characterization of a monoclonal antibody (mAb) upstream process a stepwi…
View article: Digitale Zwillinge in der Bioprozesstechnik – Chancen und Möglichkeiten
Digitale Zwillinge in der Bioprozesstechnik – Chancen und Möglichkeiten Open
Die Digitalisierung von Prozessen ist eines der aktuell dominierenden Themen und Missionen sowohl in der industriellen Produktion als auch der wissenschaftlichen Forschung. In der Biotechnologie haben diese Bemühungen ein enormes Potenzial…
View article: Optimization of a mAb production process with regard to robustness and product quality using quality by design principles
Optimization of a mAb production process with regard to robustness and product quality using quality by design principles Open
Quality by Design principles are well described and widely used in biopharmaceutical industry. The characterization of a monoclonal antibody (mAb) production process is crucial for novel process development and control. Yet, the applicatio…
View article: Green Synthesis of Silver Nanoparticles Using Hypericum perforatum L. Aqueous Extract with the Evaluation of Its Antibacterial Activity against Clinical and Food Pathogens
Green Synthesis of Silver Nanoparticles Using Hypericum perforatum L. Aqueous Extract with the Evaluation of Its Antibacterial Activity against Clinical and Food Pathogens Open
The rapid development of nanotechnology and its applications in medicine has provided the perfect solution against a wide range of different microbes, especially antibiotic-resistant ones. In this study, a one-step approach was used in pre…
View article: Identification of Major Constituents of <i>Hypericum perforatum</i> L. Extracts in Syria by Development of a Rapid, Simple, and Reproducible HPLC-ESI-Q-TOF MS Analysis and Their Antioxidant Activities
Identification of Major Constituents of <i>Hypericum perforatum</i> L. Extracts in Syria by Development of a Rapid, Simple, and Reproducible HPLC-ESI-Q-TOF MS Analysis and Their Antioxidant Activities Open
Hypericum perforatum Linn (St. John's wort) is a popular and widespread medicine in Syria, which is used for a wide range of conditions, including gastrointestinal diseases, heart disease, skin diseases, and psychological dis…
View article: Stress‐induced increase of monoclonal antibody production in CHO cells
Stress‐induced increase of monoclonal antibody production in CHO cells Open
Monoclonal antibodies (mAbs) are of great interest to the biopharmaceutical industry due to their widely used application as human therapeutic and diagnostic agents. As such, mAb require to exhibit human‐like glycolization patterns. Theref…
View article: On-Line Monitoring of Biological Parameters in Microalgal Bioprocesses Using Optical Methods
On-Line Monitoring of Biological Parameters in Microalgal Bioprocesses Using Optical Methods Open
Microalgae are promising sources of fuels and other chemicals. To operate microalgal cultivations efficiently, process control based on monitoring of process variables is needed. On-line sensing has important advantages over off-line and o…
View article: A 3D-printed microfluidic gradient generator with integrated photonic silicon sensors for rapid antimicrobial susceptibility testing
A 3D-printed microfluidic gradient generator with integrated photonic silicon sensors for rapid antimicrobial susceptibility testing Open
A 3D-printed gradient generator for automated and expeditious on-chip antimicrobial susceptibility testing in a label-free manner is presented, paving the way towards clinical point-of-care applications.
View article: Peripheral Blood Derived Endothelial Colony Forming Cells as Suitable Cell Source for Pre-Endothelialization of Arterial Vascular Grafts Under Dynamic Flow Conditions
Peripheral Blood Derived Endothelial Colony Forming Cells as Suitable Cell Source for Pre-Endothelialization of Arterial Vascular Grafts Under Dynamic Flow Conditions Open
View article: Paving the Way to Overcome Antifungal Drug Resistance: Current Practices and Novel Developments for Rapid and Reliable Antifungal Susceptibility Testing
Paving the Way to Overcome Antifungal Drug Resistance: Current Practices and Novel Developments for Rapid and Reliable Antifungal Susceptibility Testing Open
The past year has established the link between the COVID‐19 pandemic and the global spread of severe fungal infections; thus, underscoring the critical need for rapid and realizable fungal disease diagnostics. While in recent years, health…
View article: Porous Silicon-Based Aptasensors: Toward Cancer Protein Biomarker Detection
Porous Silicon-Based Aptasensors: Toward Cancer Protein Biomarker Detection Open
The anterior gradient homologue-2 (AGR2) protein is an attractive biomarker for various types of cancer. In pancreatic cancer, it is secreted to the pancreatic juice by premalignant lesions, which would be an ideal stage for diagnosis. Thu…
View article: Clinical applicability of optogenetic gene regulation
Clinical applicability of optogenetic gene regulation Open
The field of optogenetics is rapidly growing in relevance and number of developed tools. Among other things, the optogenetic repertoire includes light‐responsive ion channels and methods for gene regulation. This review will be confined to…
View article: Transferrin-Decorated Niosomes with Integrated InP/ZnS Quantum Dots and Magnetic Iron Oxide Nanoparticles: Dual Targeting and Imaging of Glioma
Transferrin-Decorated Niosomes with Integrated InP/ZnS Quantum Dots and Magnetic Iron Oxide Nanoparticles: Dual Targeting and Imaging of Glioma Open
The development of multifunctional nanoscale systems that can mediate efficient tumor targeting, together with high cellular internalization, is crucial for the diagnosis of glioma. The combination of imaging agents into one platform provi…
View article: In-Vitro Application of Magnetic Hybrid Niosomes: Targeted siRNA-Delivery for Enhanced Breast Cancer Therapy
In-Vitro Application of Magnetic Hybrid Niosomes: Targeted siRNA-Delivery for Enhanced Breast Cancer Therapy Open
Even though the administration of chemotherapeutic agents such as erlotinib is clinically established for the treatment of breast cancer, its efficiency and the therapy outcome can be greatly improved using RNA interference (RNAi) mechanis…
View article: Hypericum perforatum L.-Mediated Green Synthesis of Silver Nanoparticles Exhibiting Antioxidant and Anticancer Activities
Hypericum perforatum L.-Mediated Green Synthesis of Silver Nanoparticles Exhibiting Antioxidant and Anticancer Activities Open
This contribution focuses on the green synthesis of silver nanoparticles (AgNPs) with a size < 100 nm for potential medical applications by using silver nitrate solution and Hypericum Perforatum L. (St John’s wort) aqueous extracts. Variou…
View article: Comparative Analysis of Mesenchymal Stem Cell Cultivation in Fetal Calf Serum, Human Serum, and Platelet Lysate in 2D and 3D Systems
Comparative Analysis of Mesenchymal Stem Cell Cultivation in Fetal Calf Serum, Human Serum, and Platelet Lysate in 2D and 3D Systems Open
In vitro two-dimensional (2D) and three-dimensional (3D) cultivation of mammalian cells requires supplementation with serum. Mesenchymal stem cells (MSCs) are widely used in clinical trials for bioregenerative medicine and in most cases, i…
View article: Monitoring cell productivity for the production of recombinant proteins by flow cytometry: An effective application using the cold capture assay
Monitoring cell productivity for the production of recombinant proteins by flow cytometry: An effective application using the cold capture assay Open
Due to the increasing economic and social relevance of biotherapeutics, their production processes are continually being reconsidered and reoptimized in an effort to secure higher product concentrations and qualities. Monitoring the produc…
View article: Eine erneute Standortbestimmung: 18 Jahre nach dem Neujahrsvortrag von Professor Joachim Klein 2002 zum Thema Chemie ist Leben - (über)lebt die Chemie?
Eine erneute Standortbestimmung: 18 Jahre nach dem Neujahrsvortrag von Professor Joachim Klein 2002 zum Thema Chemie ist Leben - (über)lebt die Chemie? Open
View article: Whole-Cell Production of Patchouli Oil Sesquiterpenes in <i>Escherichia coli</i>: Metabolic Engineering and Fermentation Optimization in Solid–Liquid Phase Partitioning Cultivation
Whole-Cell Production of Patchouli Oil Sesquiterpenes in <i>Escherichia coli</i>: Metabolic Engineering and Fermentation Optimization in Solid–Liquid Phase Partitioning Cultivation Open
Patchouli oil is a major ingredient in perfumery, granting a dark-woody scent due to its main constituent (−)-patchoulol. The growing demand for patchouli oil has raised interest in the development of a biotechnological process to assure a…
View article: Implementing a digital infrastructure for the lab using a central laboratory server and the SiLA2 communication standard
Implementing a digital infrastructure for the lab using a central laboratory server and the SiLA2 communication standard Open
In this report, a fully integrated solution for laboratory digitization is presented. The approach presents a flexible and complete integration method for the digitally assisted workflow. The worker in the laboratory performs procedures in…
View article: Implementation of QbD strategies in the inoculum expansion of a mAb production process
Implementation of QbD strategies in the inoculum expansion of a mAb production process Open
The quality by design approach was introduced to the biopharmaceutical industry over 15 years ago. This principle is widely implemented in the characterization of monoclonal antibody production processes. Anyway, the early process phase, n…
View article: Clinical applicability of optogenetic gene regulation
Clinical applicability of optogenetic gene regulation Open
The field of optogenetics is rapidly growing in relevance and number of developed tools. Amongst other things, the optogenetic repertoire includes light-responsive ion channels and methods for gene regulation. This review will be confined …