Thomas H. Segall-Shapiro
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View article: Engineering a Bright Near-Infrared Fluorescent Protein by Screening a Comprehensive Phenotypic Landscape
Engineering a Bright Near-Infrared Fluorescent Protein by Screening a Comprehensive Phenotypic Landscape Open
Near-infrared fluorescent proteins (niRFPs) are valuable markers for tracking cellular phenomena as they offer greater imaging depth, lower background, and minimal invasiveness relative to other fluorescent probes. The small ultra red fluo…
View article: Directed evolution of an orthogonal transcription engine for programmable gene expression in eukaryotes
Directed evolution of an orthogonal transcription engine for programmable gene expression in eukaryotes Open
T7 RNA polymerase has enabled orthogonal control of gene expression and recombinant protein production across diverse prokaryotic host chassis organisms for decades. However, the absence of 5’ methyl guanosine caps on T7 RNAP derived trans…
View article: SARS-COV-2 Omicron variants conformationally escape a rare quaternary antibody binding mode
SARS-COV-2 Omicron variants conformationally escape a rare quaternary antibody binding mode Open
The ongoing evolution of SARS-CoV-2 into more easily transmissible and infectious variants has provided unprecedented insight into mutations enabling immune escape. Understanding how these mutations affect the dynamics of antibody-antigen …
View article: Antibody escape and cryptic cross-domain stabilization in the SARS-CoV-2 Omicron spike protein
Antibody escape and cryptic cross-domain stabilization in the SARS-CoV-2 Omicron spike protein Open
Summary The worldwide spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to the repeated emergence of variants of concern. The Omicron variant has two dominant sub-lineages, BA.1 and BA.2, each with unprecedente…
View article: Synthetic repertoires derived from convalescent COVID-19 patients enable discovery of SARS-CoV-2 neutralizing antibodies and a novel quaternary binding modality
Synthetic repertoires derived from convalescent COVID-19 patients enable discovery of SARS-CoV-2 neutralizing antibodies and a novel quaternary binding modality Open
The ongoing evolution of SARS-CoV-2 into more easily transmissible and infectious variants has sparked concern over the continued effectiveness of existing therapeutic antibodies and vaccines. Hence, together with increased genomic surveil…
View article: Marionette:<i>E. coli</i>containing 12 highly-optimized small molecule sensors
Marionette:<i>E. coli</i>containing 12 highly-optimized small molecule sensors Open
Cellular processes are carried out by many interacting genes and their study and optimization requires multiple levers by which they can be independently controlled. The most common method is via a genetically-encoded sensor that responds …
View article: Regulatory systems for the robust control of engineered genetic programs
Regulatory systems for the robust control of engineered genetic programs Open
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biological Engineering, 2017.
View article: The Structure of a Thermophilic Kinase Shapes Fitness upon Random Circular Permutation
The Structure of a Thermophilic Kinase Shapes Fitness upon Random Circular Permutation Open
Proteins can be engineered for synthetic biology through circular permutation, a sequence rearrangement in which native protein termini become linked and new termini are created elsewhere through backbone fission. However, it remains chall…