Thomas A. Trautner
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View article: A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes
A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes Open
A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes and gene products. It provides explicit categories for the many different Type II enzymes now identified and provides…
View article: Determination of Methylation Site of DNA-Methyltransferase <i>Nla</i>X by a Hybrid Method
Determination of Methylation Site of DNA-Methyltransferase <i>Nla</i>X by a Hybrid Method Open
Using a new method based on a combination of bisulfite reaction, the repair enzyme uracil-DNA glycosylase, and synthetic oligodeoxyribonucleotides, the methylation site of DNA-methyltransferase NlaX (M·NlaX) from Neisseria lactamica was es…
View article: Determination of Methylation Site of DNA-Methyltransferase <i>Nla</i> X by a Hybrid Method
Determination of Methylation Site of DNA-Methyltransferase <i>Nla</i> X by a Hybrid Method Open
Using a new method based on a combination of bisulfite reaction, the repair enzyme uracil-DNA glycosylase, and synthetic oligodeoxyribonucleotides, the methylation site of DNA-methyltransferase NlaX (M.NlaX) from Neisseria lactamica was es…
View article: M.ø3TII: a new monospecific DNA (cytosine-C5) methyltransferase with pronounced amino acid sequence similarity to a family of adenine-N6-DNA-methyltransferases
M.ø3TII: a new monospecific DNA (cytosine-C5) methyltransferase with pronounced amino acid sequence similarity to a family of adenine-N6-DNA-methyltransferases Open
The temperate B.subtilis phages phi 3T and rho 11s code, in addition to the multispecific DNA (cytosine-C5) methyltransferases (C5-MTases) M.phi 3TI and M.rho 11sI, which were previously characterized, for the identical monospecific C5-MTa…
View article: M.ø3TII: a new monospecific DNA (cytosine-C5) methyltransf erase with pronounced ami no acid sequence similarity to a family of adenine-N6-DNA-methyltransferases
M.ø3TII: a new monospecific DNA (cytosine-C5) methyltransf erase with pronounced ami no acid sequence similarity to a family of adenine-N6-DNA-methyltransferases Open
Journal Article M.ø3TII: a new monospecific DNA (cytosine-C5) methyltransf erase with pronounced ami no acid sequence similarity to a family of adenine-N6-DNA-methyltransferases Get access Mario Noyer-Weidner, Mario Noyer-Weidner Max-Planc…
View article: <i>Bsu</i>BI—an isospecific restriction and modification system of<i>Pst</i>I: characterization of the<i>Bsu</i>BI genes and enzymes
<i>Bsu</i>BI—an isospecific restriction and modification system of<i>Pst</i>I: characterization of the<i>Bsu</i>BI genes and enzymes Open
The enzymes of the Bacillus subtilis BsuBI restriction/modification (R/M) system recognize the target sequence 5'CTGCAG. The genes of the BsuBI R/M system have been cloned and sequenced and their products have been characterized following …
View article: Cloning, characterization and evolation of the<i>Bsu</i>FI restriction endonuclease gene of<i>Bacillus subtilis</i>and purification of the enzyme
Cloning, characterization and evolation of the<i>Bsu</i>FI restriction endonuclease gene of<i>Bacillus subtilis</i>and purification of the enzyme Open
The restriction endonuclease (R.BsuFI) of Bacillus subtilis recognizes the target DNA sequence 5' CCGG. The R.BsuFI gene was found in close proximity to the cognate M.BsuFI gene, which had previously been characterized (1). Cloning of the …
View article: Chimeric multispecific DNA methyltransferases with novel combinations of target recognition
Chimeric multispecific DNA methyltransferases with novel combinations of target recognition Open
DNA target recognizing domains of different multispecific DNA-cytosine-methyltransferases can be rearranged through engineering of the corresponding genes to generate enzymes with novel combinations of target recognition.
View article: Genomic organization of the related Bacillus subtilis bacteriophages SPP1, 41c, rho 15, and SF6
Genomic organization of the related Bacillus subtilis bacteriophages SPP1, 41c, rho 15, and SF6 Open
The genomes of the related virulent Bacillus subtilis bacteriophages SPP1, 41c, rho 15, and SF6 are partially circularly permuted and terminally redundant. Heteroduplex molecules were produced with various combinations of these DNAs. Their…
View article: Plasmid transduction by Bacillus subtilis bacteriophage SPP1: effects of DNA homology between plasmid and bacteriophage
Plasmid transduction by Bacillus subtilis bacteriophage SPP1: effects of DNA homology between plasmid and bacteriophage Open
Any SPP1 DNA restriction fragment cloned into Bacillus subtilis plasmid pC194 or pUB110 increased the transduction frequency of the plasmid by SPP1 100- to 1,000-fold over the transduction level of the plasmid alone. This increment was obs…
View article: Restriction and modification in Bacillus subtilis: DNA methylation potential of the related bacteriophages Z, SPR, SP beta, phi 3T, and rho 11
Restriction and modification in Bacillus subtilis: DNA methylation potential of the related bacteriophages Z, SPR, SP beta, phi 3T, and rho 11 Open
The DNA methylation capacity and some other properties of the related temperate Bacillus subtilis phages Z, SPR, SP beta, phi 3T, and rho 11 are compared. With phage mutants affected in their methylation potential, we show that phage-coded…
View article: SPP1-mediated plasmid transduction
SPP1-mediated plasmid transduction Open
The virulent Bacillus subtilis phage SPP1 transduces plasmid DNA. Plasmid-transducing phages contain only plasmid DNA. Such DNA represents a concatemer of monomeric plasmid molecules with the molecular weight of mature SPP1 DNA. Biological…
View article: Genome of <i>Bacillus subtilis</i> Bacteriophage SPP1: Structure and Nucleotide Sequence of <i>pac</i> , the Origin of DNA Packaging
Genome of <i>Bacillus subtilis</i> Bacteriophage SPP1: Structure and Nucleotide Sequence of <i>pac</i> , the Origin of DNA Packaging Open
The DNA of Bacillus subtilis bacteriophage SPP1 is terminally redundant and partially circularly permuted. To explain these parameters, we followed the Streisinger-Botstein models of phage maturation and assumed that packaging of SPP1 DNA …
View article: Restriction and modification in Bacillus subtilis: two DNA methyltransferases with BsuRI specificity. I. Purification and physical properties.
Restriction and modification in Bacillus subtilis: two DNA methyltransferases with BsuRI specificity. I. Purification and physical properties. Open
Two S-adenosyl-L-methionine:DNA (cytosine 5)-methyltransferases, termed M.BsuRIa and M.BsuRIb, were purified 3,000- and 4,000-fold, respectively, from Bacillus subtilis strain OG3R (r+m+) by successive column chromatography. The molecular …
View article: Restriction and Modification in <i>Bacillus subtilis</i> : Gene Coding for a <i>Bsu</i> R-Specific Modification Methyltransferase in the Temperate Bacteriophage Φ3T
Restriction and Modification in <i>Bacillus subtilis</i> : Gene Coding for a <i>Bsu</i> R-Specific Modification Methyltransferase in the Temperate Bacteriophage Φ3T Open
The resistance of Φ3T DNA to degradation by the restriction enzyme Bsu R or its isoschizomer Hae III is due to obligatory modification of such DNA. Biochemical and genetical experiments indicate that Φ3T codes for a methyltransferase, whic…
View article: Structure of Bacillus subtilis bacteriophage SPP1 DNA in relation to its transfection activity
Structure of Bacillus subtilis bacteriophage SPP1 DNA in relation to its transfection activity Open
The availability of a detailed restriction map of SPP1 DNA allowed defined manipulations of such molecules. These were performed to investigate structural requirements for SPP1 transfection. (i) The transfection activity of SPP1 DNA was de…
View article: Maturation of bacteriophage SPPI DNA: limited precision in the sizing of mature bacteriophage genomes
Maturation of bacteriophage SPPI DNA: limited precision in the sizing of mature bacteriophage genomes Open
Maturation of bacteriophage SPPI is imprecise. Although terminally redundant and circularly permuted molecules were always formed, individual molecules varied by more than 200 base pairs from each other.
View article: DNA methyltransferases affecting the sequence 5′LCCGG
DNA methyltransferases affecting the sequence 5′LCCGG Open
B. subtilis phage SPbeta and Moraxella sp. code for DNA methyltransferases which methylate both cytosines of the sequence 5'CCGG. Experiments using a B. subtilis strain whose DNA is sensitive to HpaII and resistant to MspI degradation, ind…
View article: Restriction and modification in Bacillus subtilis: inducibility of a DNA methylating activity in nonmodifying cells
Restriction and modification in Bacillus subtilis: inducibility of a DNA methylating activity in nonmodifying cells Open
The nonrestricting/nonmodifying strain Bacillus subtilis 222 (r-m-) can be induced to synthesize a DNA-modifying activity upon treatment with either mitomycin C (MC) or UV light. This is shown by the following facts. (i) Infection of MC-pr…
View article: ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID, X. INFLUENCE OF BROMOURACIL SUBSTITUTIONS ON REPLICATION
ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID, X. INFLUENCE OF BROMOURACIL SUBSTITUTIONS ON REPLICATION Open
Understanding a visual scene is an unsolved and daunting task, since scenes can contain a large number of objects, their properties, and interrelations. Extracting the full scene structure is therefore infeasible, but often unnecessary, si…
View article: Zur Kinetik der Rekombinantenbildung bei T 1-Bakteriophagen
Zur Kinetik der Rekombinantenbildung bei T 1-Bakteriophagen Open
A cross is made between 2 mutants of phage T 1 with a multiplicity of infection of 4 of each type. Infected bacteria are opened during the latent period after the method described by Doermann, and recombinant percentages are determined. Fr…