Thomas W. Kirby
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View article: Targeting the Structural Maturation Pathway of HIV-1 Reverse Transcriptase
Targeting the Structural Maturation Pathway of HIV-1 Reverse Transcriptase Open
Formation of active HIV-1 reverse transcriptase (RT) proceeds via a structural maturation process that involves subdomain rearrangements and formation of an asymmetric p66/p66′ homodimer. These studies were undertaken to evaluate whether t…
View article: Phosphopeptide interactions of the Nbs1 N-terminal FHA-BRCT1/2 domains
Phosphopeptide interactions of the Nbs1 N-terminal FHA-BRCT1/2 domains Open
View article: Ligand binding characteristics of the Ku80 von Willebrand domain
Ligand binding characteristics of the Ku80 von Willebrand domain Open
View article: Variations in nuclear localization strategies among pol X family enzymes
Variations in nuclear localization strategies among pol X family enzymes Open
Despite the essential roles of pol X family enzymes in DNA repair, information about the structural basis of their nuclear import is limited. Recent studies revealed the unexpected presence of a functional nuclear localization signal (NLS)…
View article: Transitions in DNA polymerase β μs-ms dynamics related to substrate binding and catalysis
Transitions in DNA polymerase β μs-ms dynamics related to substrate binding and catalysis Open
DNA polymerase β (pol β) plays a central role in the DNA base excision repair pathway and also serves as an important model polymerase. Dynamic characterization of pol β from methyl-TROSY 13C-1H multiple quantum CPMG relaxation dispersion …
View article: Ile and Met methyl 1H and 13C chemical shifts of DNA polymerase beta
Ile and Met methyl 1H and 13C chemical shifts of DNA polymerase beta Open
View article: Characterization of the APLF FHA–XRCC1 phosphopeptide interaction and its structural and functional implications
Characterization of the APLF FHA–XRCC1 phosphopeptide interaction and its structural and functional implications Open
Aprataxin and PNKP-like factor (APLF) is a DNA repair factor containing a forkhead-associated (FHA) domain that supports binding to the phosphorylated FHA domain binding motifs (FBMs) in XRCC1 and XRCC4. We have characterized the interacti…
View article: DNA polymerase β contains a functional nuclear localization signal at its N-terminus
DNA polymerase β contains a functional nuclear localization signal at its N-terminus Open
DNA polymerase β (pol β) requires nuclear localization to fulfil its DNA repair function. Although its small size has been interpreted to imply the absence of a need for active nuclear import, sequence and structural analysis suggests that…
View article: Nuclear Localization of the DNA Repair Scaffold XRCC1: Uncovering the Functional Role of a Bipartite NLS
Nuclear Localization of the DNA Repair Scaffold XRCC1: Uncovering the Functional Role of a Bipartite NLS Open
We have characterized the nuclear localization signal (NLS) of XRCC1 structurally using X-ray crystallography and functionally using fluorescence imaging. Crystallography and binding studies confirm the bipartite nature of the XRCC1 NLS in…