Christopher T. Workman
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View article: The genome of <i>Pleurosigma</i> provides insights into the evolutionary adaptations of pelagic diatoms
The genome of <i>Pleurosigma</i> provides insights into the evolutionary adaptations of pelagic diatoms Open
The diatom Pleurosigma pacificum is a newly described tropical pelagic species from the Western Pacific Ocean with one of largest genome size among published diatom genomes, making it an ideal candidate for studying adaptation to tropical …
View article: Characterizing heterologous protein burden in <i>Komagataella phaffii</i>
Characterizing heterologous protein burden in <i>Komagataella phaffii</i> Open
Yeast is a widely utilized chassis for heterologous protein production, with Komagataella phaffii well-established as a prominent nonconventional yeast in this field. Despite its widespread recognition, there remains considerable potential…
View article: Two high-quality <i>Prototheca zopfii</i> genomes provide new insights into their evolution as obligate algal heterotrophs and their pathogenicity
Two high-quality <i>Prototheca zopfii</i> genomes provide new insights into their evolution as obligate algal heterotrophs and their pathogenicity Open
The majority of the nearly 10,000 described species of green algae are photoautotrophs; however, some species have lost their ability to photosynthesize and become obligate heterotrophs that rely on parasitism for survival. Two high-qualit…
View article: Precise and versatile microplate reader-based analyses of biosensor signals from arrayed microbial colonies
Precise and versatile microplate reader-based analyses of biosensor signals from arrayed microbial colonies Open
Genetically encoded fluorescent biosensors have emerged as a powerful tool to support phenotypic screenings of microbes. Optical analyses of fluorescent sensor signals from colonies grown on solid media can be challenging as imaging device…
View article: Oral administration of helminth fluid modulates distinct tuft cell and immune‐metabolic cues linked to reduced body fat
Oral administration of helminth fluid modulates distinct tuft cell and immune‐metabolic cues linked to reduced body fat Open
Intestinal tuft cells have been shown to induce type 2 immune responses during viable parasite infections, but whether oral supplementation with a parasitic exudate is able to promote type 2 immune responses that have been shown to positiv…
View article: Large-scale changes of molecular network states explain complex traits
Large-scale changes of molecular network states explain complex traits Open
The complexity of many cellular and organismal traits results from poorly understood mechanisms integrating genetic and environmental factors via molecular networks. Here, we show when and how genetic perturbations lead to molecular change…
View article: Comparative Analysis of Sperm DNA Methylation Supports Evolutionary Acquired Epigenetic Plasticity for Organ Speciation
Comparative Analysis of Sperm DNA Methylation Supports Evolutionary Acquired Epigenetic Plasticity for Organ Speciation Open
Aim: To perform a comparative epigenomic analysis of DNA methylation in spermatozoa from humans, mice, rats and mini-pigs. Materials & methods: Genome-wide DNA methylation analysis was used to compare the methylation profiles of orthologou…
View article: Precise and versatile microplate reader-based analyses of biosensor signals from arrayed microbial colonies
Precise and versatile microplate reader-based analyses of biosensor signals from arrayed microbial colonies Open
Genetically encoded fluorescent biosensors have emerged as a powerful tool to support phenotypic screenings of microbes. Optical analyses of fluorescent sensor signals from colonies grown on solid media can be challenging as imaging device…
View article: Systematic inference of indirect transcriptional regulation by protein kinases and phosphatases
Systematic inference of indirect transcriptional regulation by protein kinases and phosphatases Open
Gene expression is controlled by pathways of regulatory factors often involving the activity of protein kinases on transcription factor proteins. Despite this well established mechanism, the number of well described pathways that include t…
View article: Emergence of phenotypically distinct subpopulations is a factor in adaptation of recombinant Saccharomyces cerevisiae under glucose-limited conditions
Emergence of phenotypically distinct subpopulations is a factor in adaptation of recombinant Saccharomyces cerevisiae under glucose-limited conditions Open
Cells cultured in a nutrient-limited environment can undergo adaptation, which confers improved fitness under long-term energy limitation. We have previously shown how a recombinant S. cerevisiae strain, producing a heterologous insulin pr…
View article: Emergence of Phenotypically Distinct Subpopulations Is a Factor in Adaptation of Recombinant Saccharomyces cerevisiae under Glucose-Limited Conditions
Emergence of Phenotypically Distinct Subpopulations Is a Factor in Adaptation of Recombinant Saccharomyces cerevisiae under Glucose-Limited Conditions Open
The yeast Saccharomyces cerevisiae is an attractive microbial host for industrial production and is used widely for manufacturing, e.g., pharmaceuticals. Chemostat cultivation mode is an efficient cultivation strategy for industrial produc…
View article: Burden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies
Burden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies Open
Production of heterologous proteins, especially biopharmaceuticals and industrial enzymes, in living cell factories consumes cellular resources. Such resources are reallocated from normal cellular processes toward production of the heterol…
View article: A2B-COVID: A Tool for Rapidly Evaluating Potential SARS-CoV-2 Transmission Events
A2B-COVID: A Tool for Rapidly Evaluating Potential SARS-CoV-2 Transmission Events Open
Identifying linked cases of infection is a critical component of the public health response to viral infectious diseases. In a clinical context, there is a need to make rapid assessments of whether cases of infection have arrived independe…
View article: Does rapid sequence divergence preclude RNA structure conservation in vertebrates?
Does rapid sequence divergence preclude RNA structure conservation in vertebrates? Open
Accelerated evolution of any portion of the genome is of significant interest, potentially signaling positive selection of phenotypic traits and adaptation. Accelerated evolution remains understudied for structured RNAs, despite the fact t…
View article: The rise of genomics in snake venom research: recent advances and future perspectives
The rise of genomics in snake venom research: recent advances and future perspectives Open
Snake venoms represent a danger to human health, but also a gold mine of bioactive proteins that can be harnessed for drug discovery purposes. The evolution of snakes and their venom has been studied for decades, particularly via tradition…
View article: Efficacy of FFP3 respirators for prevention of SARS-CoV-2 infection in healthcare workers
Efficacy of FFP3 respirators for prevention of SARS-CoV-2 infection in healthcare workers Open
Background: Respiratory protective equipment recommended in the UK for healthcare workers (HCWs) caring for patients with COVID-19 comprises a fluid-resistant surgical mask (FRSM), except in the context of aerosol generating procedures (AG…
View article: Author response: Efficacy of FFP3 respirators for prevention of SARS-CoV-2 infection in healthcare workers
Author response: Efficacy of FFP3 respirators for prevention of SARS-CoV-2 infection in healthcare workers Open
Article Figures and data Abstract Editor's evaluation Introduction Materials and methods Results Discussion Data availability References Decision letter Author response Article and author information Metrics Abstract Background: Respirator…
View article: Systematic inference of regulation by protein kinases finds surprising level of transcription factor deactivation
Systematic inference of regulation by protein kinases finds surprising level of transcription factor deactivation Open
Gene expression is controlled by pathways of regulatory factors often involving the activity of protein kinases on transcription factor proteins. Despite this well established mechanism, the number of well described pathways that include t…
View article: Superspreaders drive the largest outbreaks of hospital onset COVID-19 infections
Superspreaders drive the largest outbreaks of hospital onset COVID-19 infections Open
SARS-CoV-2 is notable both for its rapid spread, and for the heterogeneity of its patterns of transmission, with multiple published incidences of superspreading behaviour. Here, we applied a novel network reconstruction algorithm to infer …
View article: Author response: Superspreaders drive the largest outbreaks of hospital onset COVID-19 infections
Author response: Superspreaders drive the largest outbreaks of hospital onset COVID-19 infections Open
Article Figures and data Abstract eLife digest Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract SARS-CoV-2 is notable both fo…
View article: FFP3 respirators protect healthcare workers against infection with SARS-CoV-2
FFP3 respirators protect healthcare workers against infection with SARS-CoV-2 Open
IntroductionConsistent with World Health Organization (WHO) advice [1], UK Infection Protection Control guidance recommends that healthcare workers (HCWs) caring for patients with coronavirus disease 2019 (COVID-19) should use fluid resist…
View article: FFP3 respirators protect healthcare workers against infection with SARS-CoV-2
FFP3 respirators protect healthcare workers against infection with SARS-CoV-2 Open
IntroductionConsistent with World Health Organization (WHO) advice [1], UK Infection Protection Control guidance recommends that healthcare workers (HCWs) caring for patients with coronavirus disease 2019 (COVID-19) should use fluid resist…
View article: Comparative analysis of three studies measuring fluorescence from engineered bacterial genetic constructs
Comparative analysis of three studies measuring fluorescence from engineered bacterial genetic constructs Open
Reproducibility is a key challenge of synthetic biology, but the foundation of reproducibility is only as solid as the reference materials it is built upon. Here we focus on the reproducibility of fluorescence measurements from bacteria tr…
View article: Small Intestinal Tuft Cell Activity Associates With Energy Metabolism in Diet-Induced Obesity
Small Intestinal Tuft Cell Activity Associates With Energy Metabolism in Diet-Induced Obesity Open
Little is known about the involvement of type 2 immune response-promoting intestinal tuft cells in metabolic regulation. We here examined the temporal changes in small intestinal tuft cell number and activity in response to high-fat diet-i…
View article: Single-dose BNT162b2 vaccine protects against asymptomatic SARS-CoV-2 infection
Single-dose BNT162b2 vaccine protects against asymptomatic SARS-CoV-2 infection Open
The BNT162b2 mRNA COVID-19 vaccine (Pfizer-BioNTech) is being utilised internationally for mass COVID-19 vaccination. Evidence of single-dose protection against symptomatic disease has encouraged some countries to opt for delayed booster d…