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View article: Brain-derived synaptic vesicles have an intrinsic ability to sequester tubulin
Brain-derived synaptic vesicles have an intrinsic ability to sequester tubulin Open
Background The presence and function of microtubules within the synaptic bouton has long been under investigation. In recent years, evidence has accumulated that connects the synaptic vesicle cluster to the local dynamics of microtubule en…
View article: Brain-derived synaptic vesicles have an intrinsic ability to sequester tubulin
Brain-derived synaptic vesicles have an intrinsic ability to sequester tubulin Open
The presence and function of microtubules within the synaptic bouton has long been under investigation. In recent years evidence has accumulated that connects the synaptic vesicle cluster to the local dynamics of microtubule ends. Nonethel…
View article: Microtubules as a versatile reference standard for expansion microscopy
Microtubules as a versatile reference standard for expansion microscopy Open
Expansion microscopy (ExM) is continually improving, and new ExM variants need to be validated on well-defined biological structures. There is no consensus on validation structures for ExM, especially as nuclear pore complexes or DNA nanor…
View article: One-step nanoscale expansion microscopy reveals individual protein shapes
One-step nanoscale expansion microscopy reveals individual protein shapes Open
The attainable resolution of fluorescence microscopy has reached the subnanometer range, but this technique still fails to image the morphology of single proteins or small molecular complexes. Here, we expand the specimens at least tenfold…
View article: Visualizing proteins by expansion microscopy
Visualizing proteins by expansion microscopy Open
Fluorescence imaging is one of the most versatile and widely-used tools in biology 1 . Although techniques to overcome the diffraction barrier were introduced more than two decades ago, and the nominal attainable resolution kept improving …