Tiaofeng Shan
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View article: Directed evolution rice genes with randomly multiplexed sgRNAs assembly of base editors
Directed evolution rice genes with randomly multiplexed sgRNAs assembly of base editors Open
Summary CRISPR‐based directed evolution is an effective breeding biotechnology to improve agronomic traits in plants. However, its gene diversification is still limited using individual single guide RNA. We described here a multiplexed ort…
View article: Development of Plant Prime-Editing Systems for Precise Genome Editing
Development of Plant Prime-Editing Systems for Precise Genome Editing Open
Prime-editing systems have the capability to perform efficient and precise genome editing in human cells. In this study, we first developed a plant prime editor 2 (pPE2) system and test its activity by generating a targeted mutation on an …
View article: A CRISPR‐Cas9‐mediated domain‐specific base‐editing screen enables functional assessment of ACCase variants in rice
A CRISPR‐Cas9‐mediated domain‐specific base‐editing screen enables functional assessment of ACCase variants in rice Open
The evolution and practical breeding of crops depend on genetic variations. Conventionally, these variations result from natural mutations or physical and chemical mutagenesis, both of which occur randomly and lack direction. To accelerate…