Tilman E. Klassert
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View article: Evaluation of a nucleic acid preservation protocol for microbiome studies involving samples from the oral cavity
Evaluation of a nucleic acid preservation protocol for microbiome studies involving samples from the oral cavity Open
Storing oral samples at room temperature in DNA/RNA Shield buffer provides a reliable and efficient alternative to freezing for preserving microbiome samples. Furthermore, it may enhance DNA yield while preserving microbial diversity in th…
View article: Impact of Antibiotic Therapy on the Upper Respiratory Tract and Gut Mycobiome in Patients with Cystic Fibrosis
Impact of Antibiotic Therapy on the Upper Respiratory Tract and Gut Mycobiome in Patients with Cystic Fibrosis Open
Patients with cystic fibrosis (CF) are frequently exposed to antibiotic treatments, which can alter the fungal communities (mycobiome) across their mucosal sites. This pilot study investigated the impact of antibiotic exposure on the mycob…
View article: Short- and long-term impact of aseptic bathing strategies on the skin microbiome in ICU patients
Short- and long-term impact of aseptic bathing strategies on the skin microbiome in ICU patients Open
View article: Mediterranean diet component oleic acid decreases systemic impact of periodontal Porphyromonas gingivalis-infection in age: addressing role of stress resistance and microbiome
Mediterranean diet component oleic acid decreases systemic impact of periodontal Porphyromonas gingivalis-infection in age: addressing role of stress resistance and microbiome Open
Periodontitis (PD) is an age-associated infectious periodontal disease driven by dysbiosis and hyperinflammatory host response, resulting in bone and tissue loss. Often linked to systemic inflammatory comorbidities, modulating host respons…
View article: Clostridioides difficile evolution in a tertiary German hospital through a retrospective genomic characterization
Clostridioides difficile evolution in a tertiary German hospital through a retrospective genomic characterization Open
Purpose Clostridioides difficile is a major cause of healthcare-associated infections, contributing to significant morbidity and mortality. This study aimed to investigate the genomic characteristics, antimicrobial resistance (AMR) profile…
View article: Profiling the nasopharyngeal Microbiome in patients with community-acquired pneumonia caused by Streptococcus pneumoniae: diagnostic challenges and ecological insights
Profiling the nasopharyngeal Microbiome in patients with community-acquired pneumonia caused by Streptococcus pneumoniae: diagnostic challenges and ecological insights Open
Community-acquired pneumonia (CAP) is a significant health threat for adults. Although conjugate vaccines have reduced pneumococcal CAP incidence in children, Streptococcus pneumoniae -related CAP remains prevalent among older adults. The …
View article: Author response: MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
Author response: MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
View article: MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
Mycofactocin is a redox cofactor essential for the alcohol metabolism of mycobacteria. While the biosynthesis of mycofactocin is well established, the gene mftG , which encodes an oxidoreductase of the glucose-methanol-choline superfamily,…
View article: Author response: MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
Author response: MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
View article: MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
Mycofactocin is a redox cofactor essential for the alcohol metabolism of mycobacteria.. While the biosynthesis of mycofactocin is well established, the gene mftG, which encodes an oxidoreductase of the glucose-methanol-choline superfamily,…
View article: Profiling the Nasopharyngeal Microbiome in Patients with Community- Acquired Pneumonia Caused by Streptococcus pneumoniae: Diagnostic Challenges and Ecological Insights
Profiling the Nasopharyngeal Microbiome in Patients with Community- Acquired Pneumonia Caused by Streptococcus pneumoniae: Diagnostic Challenges and Ecological Insights Open
Background Community-acquired pneumonia (CAP) is a significant health threat for adults. Although conjugate vaccines have reduced pneumococcal CAP incidence in children, Streptococcus pneumoniae-related CAP remains prevalent among older ad…
View article: MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
Mycofactocin is a redox cofactor essential for the alcohol metabolism of Mycobacteria. While the biosynthesis of mycofactocin is well established, the gene mftG, which encodes an oxidoreductase of the glucose-methanol-choline superfamily, …
View article: Author response: MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
Author response: MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
View article: Reviewer #2 (Public Review): MftG is crucial for alcohol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
Reviewer #2 (Public Review): MftG is crucial for alcohol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
Mycofactocin is a redox cofactor essential for the alcohol metabolism of Mycobacteria. While the biosynthesis of mycofactocin is well established, the gene mftG, which encodes an oxidoreductase of the glucose-methanol-choline superfamily, …
View article: MftG is crucial for alcohol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
MftG is crucial for alcohol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
Mycofactocin is a redox cofactor essential for the alcohol metabolism of Mycobacteria. While the biosynthesis of mycofactocin is well established, the gene mftG, which encodes an oxidoreductase of the glucose-methanol-choline superfamily, …
View article: MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
Mycofactocin is a redox cofactor essential for the alcohol metabolism of mycobacteria. While the biosynthesis of mycofactocin is well established, the gene mftG , which encodes an oxidoreductase of the glucose-methanol-choline superfamily,…
View article: Reviewer #1 (Public Review): MftG is crucial for alcohol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
Reviewer #1 (Public Review): MftG is crucial for alcohol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
Mycofactocin is a redox cofactor essential for the alcohol metabolism of Mycobacteria. While the biosynthesis of mycofactocin is well established, the gene mftG, which encodes an oxidoreductase of the glucose-methanol-choline superfamily, …
View article: MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
Mycofactocin is a redox cofactor essential for the alcohol metabolism of mycobacteria.. While the biosynthesis of mycofactocin is well established, the gene mftG , which encodes an oxidoreductase of the glucose-methanol-choline superfamily…
View article: Skin dysbiosis and loss of microbiome site specificity in critically ill patients
Skin dysbiosis and loss of microbiome site specificity in critically ill patients Open
An increasing amount of evidence has linked critical illness with dysbiotic microbiome signatures in different body sites. The disturbance of the indigenous microbiota structures has been further associated with disease severity and outcom…
View article: Role of Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in inflammation and pathogen-associated interactions
Role of Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in inflammation and pathogen-associated interactions Open
Background: Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is known as a major receptor for oxidized low-density lipoproteins (oxLDL) and plays a significant role in the genesis of atherosclerosis. Recent research has show…
View article: Competitive inhibition and mutualistic growth in co-infections: deciphering <i>Staphylococcus aureus–Acinetobacter baumannii</i> interaction dynamics
Competitive inhibition and mutualistic growth in co-infections: deciphering <i>Staphylococcus aureus–Acinetobacter baumannii</i> interaction dynamics Open
Staphylococcus aureus (Sa) and Acinetobacter baumannii (Ab) are frequently co-isolated from polymicrobial infections that are severe and refractory to therapy. Here, we apply a combination of wet-lab experiments and in silico modeling to u…
View article: Differential Transcriptional Responses of Human Granulocytes to Fungal Infection with Candida albicans and Aspergillus fumigatus
Differential Transcriptional Responses of Human Granulocytes to Fungal Infection with Candida albicans and Aspergillus fumigatus Open
Neutrophils are critical phagocytic cells in innate immunity, playing a significant role in defending against invasive fungal pathogens. This study aimed to explore the transcriptional activation of human neutrophils in response to differe…
View article: Microbiome and immuno-metabolic dysregulation in patients with major depressive disorder with atypical clinical presentation
Microbiome and immuno-metabolic dysregulation in patients with major depressive disorder with atypical clinical presentation Open
Depression is highly prevalent (6% 1-year prevalence) and is the second leading cause of disability worldwide. Available treatment options for depression are far from optimal, with response rates only around 50%. This is most likely relate…
View article: Extraction of High-Quality RNA from S. aureus Internalized by Endothelial Cells
Extraction of High-Quality RNA from S. aureus Internalized by Endothelial Cells Open
Staphylococcus aureus evades antibiotic therapy and antimicrobial defenses by entering human host cells. Bacterial transcriptomic analysis represents an invaluable tool to unravel the complex interplay between host and pathogen. Therefore,…
View article: Staphylococcus aureus induces tolerance in human monocytes accompanied with expression changes of cell surface markers
Staphylococcus aureus induces tolerance in human monocytes accompanied with expression changes of cell surface markers Open
Exposure of human monocytes to lipopolysaccharide (LPS) or other pathogen-associated molecular pattern (PAMPs) induces a temporary insensitivity to subsequent LPS challenges, a cellular state called endotoxin tolerance (ET), associated wit…
View article: Comparative analysis of surface sanitization protocols on the bacterial community structures in the hospital environment
Comparative analysis of surface sanitization protocols on the bacterial community structures in the hospital environment Open
View article: Comparative analysis of surface sanitization protocols on the bacterial community structures in the hospital environment
Comparative analysis of surface sanitization protocols on the bacterial community structures in the hospital environment Open
In this study, we used 16S rRNA gene sequencing approaches to characterize the bacterial microbiota on different surfaces of the hospital environment. The longitudinal data was then subjected to comprehensive comparisons between different …
View article: Comparative analysis of surface sanitization protocols on the bacterial community structures in the hospital environment
Comparative analysis of surface sanitization protocols on the bacterial community structures in the hospital environment Open
In this study, we used 16S rRNA gene sequencing approaches to characterize the bacterial microbiota on different surfaces of the hospital environment. The longitudinal data was then subjected to comprehensive comparisons between different …
View article: Antibody ligation of CEACAM1, CEACAM3, and CEACAM6, differentially enhance the cytokine release of human neutrophils in responses to Candida albicans
Antibody ligation of CEACAM1, CEACAM3, and CEACAM6, differentially enhance the cytokine release of human neutrophils in responses to Candida albicans Open
View article: Inhibition of the NLRP3/IL‐1β axis protects against sepsis‐induced cardiomyopathy
Inhibition of the NLRP3/IL‐1β axis protects against sepsis‐induced cardiomyopathy Open
Background Septic cardiomyopathy worsens the prognosis of critically ill patients. Clinical data suggest that interleukin‐1β (IL‐1β), activated by the NLRP3 inflammasome, compromises cardiac function. Whether or not deleting Nlrp3 would pr…