Tim Causon
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View article: Online Monitoring of Chip-Based Microscale Perfusion Fermentations
Online Monitoring of Chip-Based Microscale Perfusion Fermentations Open
As part of established biomanufacturing development, screening and early phase bioprocess development occurs at bench scale (microplates and shake flasks) whereby conventional offline sampling can only provide limited feedback on fermentat…
View article: Coproheme decarboxylase from Bacillus subtilis is required for bacterial growth and heme b biosynthesis under anaerobic conditions
Coproheme decarboxylase from Bacillus subtilis is required for bacterial growth and heme b biosynthesis under anaerobic conditions Open
Heme biosynthesis notably does not follow a universal pathway. Instead, different organisms utilize various routes of producing this essential molecule. The coproporphyrin-dependent (CPD) pathway is unique to Gram-positive bacteria. Given …
View article: Unraveling zinc acquisition strategies in barley: the role of phytosiderophore exudation and root-associated microbiome
Unraveling zinc acquisition strategies in barley: the role of phytosiderophore exudation and root-associated microbiome Open
A nutritious and healthy diet relies on consuming cereals with sufficient micronutrient content, making the production of nutrient-rich crops a crucial agricultural goal. Identifying key strategies for plant micronutrient acquisition is es…
View article: Wanted: Micronutrients – Exploring the phytosiderophore pathway for micronutrient acquisition in plant-soil systems
Wanted: Micronutrients – Exploring the phytosiderophore pathway for micronutrient acquisition in plant-soil systems Open
Micronutrient (MN) deficiencies, particularly of iron (Fe), zinc (Zn), and copper (Cu), are major constraints to crop productivity in arid and semi-arid regions characterized by high-pH calcareous soils. Effective strategies for acquiring …
View article: Engineering Komagataella phaffii for citric acid production through carbon-conserving supply of acetyl-CoA
Engineering Komagataella phaffii for citric acid production through carbon-conserving supply of acetyl-CoA Open
The oxidative formation of AcCoA limits the glycolytic pathway yield (YPGLY) for citric acid due to the NADH overflow and carbon loss as CO2. An interesting approach to enhance product yields is the incorporation of carbon-conserving pathw…
View article: Computational and Experimental IM-MS Determination of the Protonated Structures of Antimalarial Drugs
Computational and Experimental IM-MS Determination of the Protonated Structures of Antimalarial Drugs Open
A combination of ion mobility-mass spectrometry (IM-MS) measurements and computational methods were used to study structural and physicochemical properties of a range of quinoline-based drugs: amodiaquine (AQ), cinchonine (CIN), chloroquin…
View article: CCSfind: A tool for chemically informed LC‐IM‐MS database building
CCSfind: A tool for chemically informed LC‐IM‐MS database building Open
In addition to providing critical knowledge of the accurate mass of ions, ion mobility‐mass spectrometry (IM‐MS) delivers complementary data relating to the conformation and size of ions in the form of an ion mobility spectrum and derived …
View article: MobiLipid: A Tool for Enhancing CCS Quality Control of Ion Mobility–Mass Spectrometry Lipidomics by Internal Standardization
MobiLipid: A Tool for Enhancing CCS Quality Control of Ion Mobility–Mass Spectrometry Lipidomics by Internal Standardization Open
Ion mobility-mass spectrometry (IM-MS) offers benefits for lipidomics by obtaining IM-derived collision cross sections (CCS), a conditional property of an ion that can enhance lipid identification. While drift tube (DT) IM-MS retains a dir…
View article: MobiLipid: A Tool for Enhancing CCS Quality Control of Ion Mobility-Mass Spectrometry Lipidomics by Internal Standardization
MobiLipid: A Tool for Enhancing CCS Quality Control of Ion Mobility-Mass Spectrometry Lipidomics by Internal Standardization Open
Ion mobility-mass spectrometry (IM-MS) offers benefits for lipidomics by obtaining IM-derived collision cross sections (CCS), a conditional physicochemical parameter of an ion which can enhance lipid identification. While drift tube (DT) I…
View article: CCSfind: A tool for chemically informed LC-IM-MS database building
CCSfind: A tool for chemically informed LC-IM-MS database building Open
In addition to providing critical knowledge of the accurate mass of ions, ion mobility-mass spectrometry (IM-MS) delivers complementary data relating to the conformation and size of ions in the form of an ion mobility spectrum and derived …
View article: Three-Minute Enantioselective Amino Acid Analysis by Ultra-High-Performance Liquid Chromatography Drift Tube Ion Mobility-Mass Spectrometry Using a Chiral Core–Shell Tandem Column Approach
Three-Minute Enantioselective Amino Acid Analysis by Ultra-High-Performance Liquid Chromatography Drift Tube Ion Mobility-Mass Spectrometry Using a Chiral Core–Shell Tandem Column Approach Open
Fast liquid chromatography (LC) amino acid enantiomer separation of 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatives using a chiral core-shell particle tandem column with weak anion exchange and zwitterionic-type quinine c…
View article: Phytosiderophore pathway response in barley exposed to iron, zinc or copper starvation
Phytosiderophore pathway response in barley exposed to iron, zinc or copper starvation Open
Efficient micronutrient acquisition is a critical factor in selecting micronutrient dense crops for human consumption. Enhanced exudation and re-uptake of metal chelators, so-called phytosiderophores, by roots of graminaceous plants has be…
View article: Surveying the mugineic acid family: Ion mobility – quadrupole time-of-flight mass spectrometry (IM-QTOFMS) characterization and tandem mass spectrometry (LC-ESI-MS/MS) quantification of all eight naturally occurring phytosiderophores
Surveying the mugineic acid family: Ion mobility – quadrupole time-of-flight mass spectrometry (IM-QTOFMS) characterization and tandem mass spectrometry (LC-ESI-MS/MS) quantification of all eight naturally occurring phytosiderophores Open
Phytosiderophores (PS) are root exudates released by grass species (Poaceae) that play a pivotal role in iron (Fe) plant nutrition. A direct determination of PS in biological samples is of paramount importance in understanding micronutrien…
View article: Ultrathin membranes composed of branched polyethylenimine and poly[(o-cresyl glycidyl ether)-co-formaldehyde] for primary recovery of itaconic acid
Ultrathin membranes composed of branched polyethylenimine and poly[(o-cresyl glycidyl ether)-co-formaldehyde] for primary recovery of itaconic acid Open
Efficient primary recovery of building-block chemicals produced by microbial processes, such as biobased itaconic acid, is a key step for environmentally sustainable and economically feasible manufacturing processes. Ultrathin membranes ha…
View article: Metabolic composition of healthy and infected Grüner Veltliner grapes and wine assessed by high resolution mass spectrometry
Metabolic composition of healthy and infected Grüner Veltliner grapes and wine assessed by high resolution mass spectrometry Open
Grapevines are susceptible to a wide range of fungal diseases that result in significant annual economic losses in the global wine industry. The present study uses a non-targeted mass spectrometry approach to explore metabolic profiles of …
View article: Establishing carbon–carbon double bond position and configuration in unsaturated fatty acids by gas-phase infrared spectroscopy
Establishing carbon–carbon double bond position and configuration in unsaturated fatty acids by gas-phase infrared spectroscopy Open
3-Pyridylcarbinol ester derivatization introduces a universal probe for cryogenic gas-phase infrared spectroscopy of fatty acids that enables assignment of the position and configuration of carbon–carbon double bonds even in the presence o…
View article: Comparability of Steroid Collision Cross Sections Using Three Different IM-HRMS Technologies: An Interplatform Study
Comparability of Steroid Collision Cross Sections Using Three Different IM-HRMS Technologies: An Interplatform Study Open
International audience
View article: Critical evaluation of the role of external calibration strategies for IM-MS
Critical evaluation of the role of external calibration strategies for IM-MS Open
The major benefits of integrating ion mobility (IM) into LC–MS methods for small molecules are the additional separation dimension and especially the use of IM-derived collision cross sections ( CCS ) as an additional ion-specific identifi…
View article: Comparability of Steroid Collision Cross Sections Using Three Different IM-HRMS Technologies: An Interplatform Study
Comparability of Steroid Collision Cross Sections Using Three Different IM-HRMS Technologies: An Interplatform Study Open
Steroids play key roles in various biological processes and are characterized by many isomeric variants which makes their unambiguous identification challenging. Ion mobility-mass spectrometry (IM-MS) has been proposed as a suitable platfo…
View article: Significance of Competitive Reactions in an Atmospheric Pressure Chemical Ionization Ion Source: Effect of Solvent
Significance of Competitive Reactions in an Atmospheric Pressure Chemical Ionization Ion Source: Effect of Solvent Open
Ionization of organic compounds with different structural and energetic properties including benzene derivatives, polycyclic aromatic hydrocarbons (PAHs), ketones, and polyenes was studied using a commercial atmospheric pressure corona dis…
View article: Non-targeted analysis with high-resolution mass spectrometry for investigation of riverbank filtration processes
Non-targeted analysis with high-resolution mass spectrometry for investigation of riverbank filtration processes Open
A fully non-targeted analytical workflow for the investigation of a riverbank filtration site located at the river Danube has been developed and applied. Variations of compound intensities at different sampling locations of the riverbank f…
View article: Combining iron affinity-based fractionation with non-targeted LC-ESI-TOFMS for the study of iron-binding molecules in dissolved organic matter
Combining iron affinity-based fractionation with non-targeted LC-ESI-TOFMS for the study of iron-binding molecules in dissolved organic matter Open
The low solubility of inorganic iron(III) in seawater leads to very limited availability of this important micronutrient for marine organisms. Estuarine or oceanic iron is almost entirely bound to organic ligands of mainly unknown chemical…
View article: Alternating in-source fragmentation with single-stage high-resolution mass spectrometry with high annotation confidence in non-targeted metabolomics
Alternating in-source fragmentation with single-stage high-resolution mass spectrometry with high annotation confidence in non-targeted metabolomics Open
Non-targeted metabolomics is increasingly applied in various applications for understanding biological processes and finding novel biomarkers in living organisms. However, high-confidence identity confirmation of metabolites in complex bio…
View article: Plate‐height model of ion mobility‐mass spectrometry: Part 2—Peak‐to‐peak resolution and peak capacity
Plate‐height model of ion mobility‐mass spectrometry: Part 2—Peak‐to‐peak resolution and peak capacity Open
In a previous work, we explored zone broadening and the achievable plate numbers in linear drift tube ion mobility‐mass spectrometry through developing a plate‐height model [1]. On the basis of these findings, the present theoretical study…
View article: Novel acquisition strategies for metabolomics using drift tube ion mobility-quadrupole resolved all ions time-of-flight mass spectrometry (IM-QRAI-TOFMS)
Novel acquisition strategies for metabolomics using drift tube ion mobility-quadrupole resolved all ions time-of-flight mass spectrometry (IM-QRAI-TOFMS) Open
The focus of this work was the implementation of ion mobility (IM) and a prototype quadrupole driver within data independent acquisition (DIA) using a drift tube IM-QTOFMS aiming to improve the level of confidence in identity confirmation …