The oxidative formation of AcCoA limits the glycolytic pathway yield (Y^P_GLY) for citric acid due to the NADH overflow and carbon loss as CO₂. An interesting approach to enhance product yields is the incorporation of carbon-conserving pathways. This study assesses the potential of a carbon-conserving AcCoA pathway, the glycolysis alternative high carbon yield cycle (GATHCYC), to improve citric acid production, utilizing the non-native citric acid producer Komagataella phaffii as an …

As part of established biomanufacturing development, screening and early phase bioprocess development occurs at bench scale (microplates and shake flasks) whereby conventional offline sampling can only provide limited feedback on fermentation bioprocess parameters including strain productivity. To address these limitations, a new sensitive and selective online analytical platform consisting entirely of commercially available components with a small footprint (valves, 2DLC hardware, LC separation, and online tandem…

Heme biosynthesis notably does not follow a universal pathway. Instead, different organisms utilize various routes of producing this essential molecule. The coproporphyrin-dependent (CPD) pathway is unique to Gram-positive bacteria. Given the ubiquity of Gram-positive pathogenic organisms, thorough research on its enzymatic steps is a prerequisite for the development of novel antibiotics. Here the focus lies on coproheme decarboxylase (ChdC, formerly HemQ), the terminal step of the pathway, catalyzing the transfor…

A nutritious and healthy diet relies on consuming cereals with sufficient micronutrient content, making the production of nutrient-rich crops a crucial agricultural goal. Identifying key strategies for plant micronutrient acquisition is essential, and this is especially critical for regions where calcareous or nutrient-deficient soils limit micronutrient bioavailability.Plants can enhance nutrient uptake by increasing exploration of the soil volume through root growth or by releasing root exudates that facilitate …

Micronutrient (MN) deficiencies, particularly of iron (Fe), zinc (Zn), and copper (Cu), are major constraints to crop productivity in arid and semi-arid regions characterized by high-pH calcareous soils. Effective strategies for acquiring MN are essential to ensure high yields on nutrient-depleted soils and produce MN-rich crops. In the case of iron, grass species (Poaceae) increase Fe phytoavailability by releasing root exudates called phytosiderophores (PS), which have the capability to chelate and mobilize Fe f…