Tim Hohmann
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View article: Identification and Characterization of SRSF2 as a Splicing-Relevant Factor Associated with the Distribution of Membranous to Secreted PD-L1, Exemplarily Considered on Human Renal Tissue, Including Renal Cell Carcinoma
Identification and Characterization of SRSF2 as a Splicing-Relevant Factor Associated with the Distribution of Membranous to Secreted PD-L1, Exemplarily Considered on Human Renal Tissue, Including Renal Cell Carcinoma Open
Background: The immunomodulatory molecule PD-L1 and its immunological tolerance-mediating interaction with the PD-1 receptor on many immune effector cells represent one of the most important tumor immune checkpoint axes in antibody-based a…
View article: P12.34.A TRACKING INDIVIDUAL AND COLLECTIVE CELLULAR DIFFUSION IN PATIENT-DERIVED PRIMARY GBM CELL LINES: EXPLORING CELL DIFFUSION PROPERTIES AS A POTENTIAL VULNERABILITY IN GLIOBLASTOMA
P12.34.A TRACKING INDIVIDUAL AND COLLECTIVE CELLULAR DIFFUSION IN PATIENT-DERIVED PRIMARY GBM CELL LINES: EXPLORING CELL DIFFUSION PROPERTIES AS A POTENTIAL VULNERABILITY IN GLIOBLASTOMA Open
BACKGROUND Glioblastoma (GBM) is one of the most aggressive and deadly brain tumors, with a median survival of ~14 months. Its poor prognosis is largely due to its ability to infiltrate healthy brain tissue both as single cells and in coll…
View article: Identification and Characterization of the RNA Modifying Factors PUS7 and WTAP as Key Components for the Control of Tumor Biological Processes in Renal Cell Carcinomas
Identification and Characterization of the RNA Modifying Factors PUS7 and WTAP as Key Components for the Control of Tumor Biological Processes in Renal Cell Carcinomas Open
Current research discusses the putative importance of RNA modification in tumor diseases. These RNA modifications include predominantly pseudouridinylation, ortho-methylations on the ribose residues, as well as methylations on the organic …
View article: RNA-Sequenzierung zur Analyse Epstein-Barr-Virus-codierter microRNAs
RNA-Sequenzierung zur Analyse Epstein-Barr-Virus-codierter microRNAs Open
The RNA species of microRNAs represents an important component of post-transcriptional gene regulation. Herpes viruses, including the Epstein-Barr virus, encode their own microRNA genes, which play a relevant role in crucial cellular proce…
View article: Analyzing the Impact of the Highest Expressed Epstein–Barr Virus-Encoded microRNAs on the Host Cell Transcriptome
Analyzing the Impact of the Highest Expressed Epstein–Barr Virus-Encoded microRNAs on the Host Cell Transcriptome Open
The Epstein–Barr virus (EBV) has a very high prevalence (>90% in adults), establishes a lifelong latency after primary infection, and exerts an oncogenic potential. This dsDNA virus encodes for various molecules, including microRNAs (miRs)…
View article: Elucidation of GPR55-Associated Signaling behind THC and LPI Reducing Effects on Ki67-Immunoreactive Nuclei in Patient-Derived Glioblastoma Cells
Elucidation of GPR55-Associated Signaling behind THC and LPI Reducing Effects on Ki67-Immunoreactive Nuclei in Patient-Derived Glioblastoma Cells Open
GPR55 is involved in many physiological and pathological processes. In cancer, GPR55 has been described to show accelerating and decelerating effects in tumor progression resulting from distinct intracellular signaling pathways. GPR55 beco…
View article: A toolbox to analyze collective cell migration, proliferation and cellular organization simultaneously
A toolbox to analyze collective cell migration, proliferation and cellular organization simultaneously Open
The presented tool allows the simultaneous analysis of collective cell behavior from phase contrast images in terms of migration, (self-)organization and proliferation.
View article: MACC1-induced migration in tumors: Current state and perspective
MACC1-induced migration in tumors: Current state and perspective Open
Malignant tumors are still a global, heavy health burden. Many tumor types cannot be treated curatively, underlining the need for new treatment targets. In recent years, metastasis associated in colon cancer 1 (MACC1) was identified as a p…
View article: A toolbox to analyze collective cell migration, proliferation and cellular organization simultaneously
A toolbox to analyze collective cell migration, proliferation and cellular organization simultaneously Open
Analyses of collective cell migration and orientation phenomena are needed to assess the behavior of multicellular clusters. While some tools to the authors’ knowledge none is capable to analyze collective migration, cellular orientation a…
View article: Jamming Transitions in Astrocytes and Glioblastoma Are Induced by Cell Density and Tension
Jamming Transitions in Astrocytes and Glioblastoma Are Induced by Cell Density and Tension Open
Collective behavior of cells emerges from coordination of cell–cell-interactions and is important to wound healing, embryonic and tumor development. Depending on cell density and cell–cell interactions, a transition from a migratory, fluid…
View article: Jamming transitions in astrocytes and glioblastoma are induced by cell density and tension
Jamming transitions in astrocytes and glioblastoma are induced by cell density and tension Open
Supplemental to the associated manuscript
View article: (Un-)jJamming transitions in astrocytes and glioblastoma
(Un-)jJamming transitions in astrocytes and glioblastoma Open
Supplemental Material to the manuscript entitled (Un-)jamming transitions in astrocytes and glioblastoma
View article: MACC1-Induced Collective Migration Is Promoted by Proliferation Rather Than Single Cell Biomechanics
MACC1-Induced Collective Migration Is Promoted by Proliferation Rather Than Single Cell Biomechanics Open
Metastasis-associated in colon cancer 1 (MACC1) is a marker for metastasis, tumor cell migration, and increased proliferation in colorectal cancer (CRC). Tumors with high MACC1 expression show a worse prognosis and higher invasion into nei…
View article: Microglia-Dependent and Independent Brain Cytoprotective Effects of Mycophenolate Mofetil During Neuronal Damage
Microglia-Dependent and Independent Brain Cytoprotective Effects of Mycophenolate Mofetil During Neuronal Damage Open
Acute lesions of the central nervous system often lead to permanent limiting deficits. In addition to the initial primary damage, accompanying neuroinflammation is responsible for progression of damage. Mycophenolate mofetil (MMF) as a sel…
View article: Nimodipine Exerts Time-Dependent Neuroprotective Effect after Excitotoxical Damage in Organotypic Slice Cultures
Nimodipine Exerts Time-Dependent Neuroprotective Effect after Excitotoxical Damage in Organotypic Slice Cultures Open
During injuries in the central nervous system, intrinsic protective processes become activated. However, cellular reactions, especially those of glia cells, are frequently unsatisfactory, and further exogenous protective mechanisms are nec…
View article: A computed tomography based survey study investigating the agreement of the therapeutic strategy for fragility fractures of the pelvis
A computed tomography based survey study investigating the agreement of the therapeutic strategy for fragility fractures of the pelvis Open
Treatment recommendations for fragility fractures of the pelvis (FFP) have been provided along with the good reliable FFP classification but they are not proven in large studies and recent reports challenge these recommendations. Thus, we …
View article: Interaction of Glia Cells with Glioblastoma and Melanoma Cells under the Influence of Phytocannabinoids
Interaction of Glia Cells with Glioblastoma and Melanoma Cells under the Influence of Phytocannabinoids Open
Brain tumor heterogeneity and progression are subject to complex interactions between tumor cells and their microenvironment. Glioblastoma and brain metastasis can contain 30–40% of tumor-associated macrophages, microglia, and astrocytes, …
View article: Variants of Oxidized Regenerated Cellulose and Their Distinct Effects on Neuronal Tissue
Variants of Oxidized Regenerated Cellulose and Their Distinct Effects on Neuronal Tissue Open
Based on oxidized regenerated cellulose (ORC), several hemostyptic materials, such as Tabotamp®, Equicel® and Equitamp®, have been developed to approach challenging hemostasis in neurosurgery. The present study compares ORC that differ in …
View article: Assessment of Neuronal Damage in Brain Slice Cultures Using Machine Learning Based on Spatial Features
Assessment of Neuronal Damage in Brain Slice Cultures Using Machine Learning Based on Spatial Features Open
Neuronal damage presents a major health issue necessitating extensive research to identify mechanisms of neuronal cell death and potential therapeutic targets. Commonly used models are slice cultures out of different brain regions extracte…
View article: THC Reduces Ki67-Immunoreactive Cells Derived from Human Primary Glioblastoma in a GPR55-Dependent Manner
THC Reduces Ki67-Immunoreactive Cells Derived from Human Primary Glioblastoma in a GPR55-Dependent Manner Open
Glioblastoma (GBM) is the most frequent malignant tumor of the central nervous system in humans with a median survival time of less than 15 months. ∆9-Tetrahydrocannabinol (THC) and cannabidiol (CBD) are the best-characterized components o…
View article: Evaluation of machine learning models for automatic detection of DNA double strand breaks after irradiation using a γH2AX foci assay
Evaluation of machine learning models for automatic detection of DNA double strand breaks after irradiation using a γH2AX foci assay Open
Ionizing radiation induces amongst other the most critical type of DNA damage: double-strand breaks (DSBs). Efficient repair of such damage is crucial for cell survival and genomic stability. The analysis of DSB associated foci assays is o…
View article: Abnormal Cannabidiol Affects Production of Pro-Inflammatory Mediators and Astrocyte Wound Closure in Primary Astrocytic-Microglial Cocultures
Abnormal Cannabidiol Affects Production of Pro-Inflammatory Mediators and Astrocyte Wound Closure in Primary Astrocytic-Microglial Cocultures Open
Abnormal cannabidiol (abn-CBD) exerts neuroprotective effects in vivo and in vitro. In the present study, we investigated the impact of abn-CBD on the glial production of proinflammatory mediators and scar formation within in vitro models.…
View article: Opposite Effects of Neuroprotective Cannabinoids, Palmitoylethanolamide, and 2-Arachidonoylglycerol on Function and Morphology of Microglia
Opposite Effects of Neuroprotective Cannabinoids, Palmitoylethanolamide, and 2-Arachidonoylglycerol on Function and Morphology of Microglia Open
Various studies performed in cultured cells and in in vivo models of neuronal damage showed that cannabinoids exert a neuroprotective effect. The increase in cannabinoids and cannabinoid like substances after stroke has been postulated to …
View article: Radiosensitization and a Less Aggressive Phenotype of Human Malignant Glioma Cells Expressing Isocitrate Dehydrogenase 1 (IDH1) Mutant Protein: Dissecting the Mechanisms
Radiosensitization and a Less Aggressive Phenotype of Human Malignant Glioma Cells Expressing Isocitrate Dehydrogenase 1 (IDH1) Mutant Protein: Dissecting the Mechanisms Open
The presence of an isocitrate dehydrogenase 1 (IDH1) mutation is associated with a less aggressive phenotype, increased sensitivity to radiation, and increased overall survival in patients with diffuse glioma. Based on in vitro experimenta…
View article: The Cytoskeleton—A Complex Interacting Meshwork
The Cytoskeleton—A Complex Interacting Meshwork Open
The cytoskeleton of animal cells is one of the most complicated and functionally versatile structures, involved in processes such as endocytosis, cell division, intra-cellular transport, motility, force transmission, reaction to external f…
View article: Protective Effect of N-Arachidonoyl Glycine-GPR18 Signaling after Excitotoxical Lesion in Murine Organotypic Hippocampal Slice Cultures
Protective Effect of N-Arachidonoyl Glycine-GPR18 Signaling after Excitotoxical Lesion in Murine Organotypic Hippocampal Slice Cultures Open
N-arachidonoyl glycine (NAGly) is an endocannabinoid involved in the regulation of different immune cells. It was shown to activate the GPR18 receptor, which was postulated to switch macrophages from cytotoxic to reparative. To study GPR18…
View article: On the influence of cannabinoids on cell morphology and motility of glioblastoma cells
On the influence of cannabinoids on cell morphology and motility of glioblastoma cells Open
The mechanisms behind the anti-tumoral effects of cannabinoids by impacting the migratory activity of tumor cells are only partially understood. Previous studies demonstrated that cannabinoids altered the organization of the actin cytoskel…
View article: The Impact of Non-Lethal Single-Dose Radiation on Tumor Invasion and Cytoskeletal Properties
The Impact of Non-Lethal Single-Dose Radiation on Tumor Invasion and Cytoskeletal Properties Open
Irradiation is the standard therapy for glioblastoma multiforme. Glioblastoma are highly resistant to radiotherapy and the underlying mechanisms remain unclear. To better understand the biological effects of irradiation on glioblastoma cel…
View article: Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells
Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells Open
In organotypic hippocampal slice cultures (OHSC), the morphological and functional characteristics of both neurons and glial cells are well preserved. This model is suitable for addressing different research questions that involve studies …