Timothy A. Springer
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View article: A single-chain derivative of an integrin-activating antibody potentiates organoid growth in Matrigel and collagen hydrogels
A single-chain derivative of an integrin-activating antibody potentiates organoid growth in Matrigel and collagen hydrogels Open
Current methods of culturing human epithelial organoids from adult stem cells may not be compatible with clinical applications as they rely on xenogeneic, chemically undefined or non-standardized components such as the basement membrane ex…
View article: Nanobodies targeting EGFR provide insight into conformations stabilized by glioblastoma mutations
Nanobodies targeting EGFR provide insight into conformations stabilized by glioblastoma mutations Open
Oncogenic mutations in the epidermal growth factor receptor (EGFR) promote tumorigenesis by stabilizing active or pre-active receptor conformations. Most EGFR-driven cancers are characterized by kinase domain mutations that directly activa…
View article: De Novo Design of Integrin α5β1 Modulating Proteins to Enhance Biomaterial Properties
De Novo Design of Integrin α5β1 Modulating Proteins to Enhance Biomaterial Properties Open
Integrin α5β1 is crucial for cell attachment and migration in development and tissue regeneration, and α5β1 binding proteins can have considerable utility in regenerative medicine and next‐generation therapeutics. We use computational prot…
View article: High-Throughput Machine Learning-Aided Antibody Discovery for Cell Surface Antigens
High-Throughput Machine Learning-Aided Antibody Discovery for Cell Surface Antigens Open
Machine learning (ML) has the potential to revolutionize antibody design and selection, but its success depends on access to extensive, well-curated datasets of antibody-antigen interactions. To address this need, we developed a synthetic …
View article: De Novo Design of Integrin α5β1 Modulating Proteins for Regenerative Medicine
De Novo Design of Integrin α5β1 Modulating Proteins for Regenerative Medicine Open
Summary Integrin α5β1 is crucial for cell attachment and migration in development and tissue regeneration, and α5β1 binding proteins could have considerable utility in regenerative medicine and next-generation therapeutics. We use computat…
View article: Synthetic integrin antibodies discovered by yeast display reveal αV subunit pairing preferences with β subunits
Synthetic integrin antibodies discovered by yeast display reveal αV subunit pairing preferences with β subunits Open
Integrins are cell surface receptors that mediate the interactions of cells with their surroundings and play essential roles in cell adhesion, migration, and homeostasis. Eight of the 24 integrins bind to the tripeptide Arg-Gly-Asp (RGD) m…
View article: Ligand binding initiates single-molecule integrin conformational activation
Ligand binding initiates single-molecule integrin conformational activation Open
View article: Synthetic integrin antibodies discovered by yeast display reveal αV subunit pairing preferences with β subunits
Synthetic integrin antibodies discovered by yeast display reveal αV subunit pairing preferences with β subunits Open
Eight of the 24 integrin heterodimers bind to the tripeptide Arg-Gly-Asp (RGD) motif in their extracellular ligands, and play essential roles in cell adhesion, migration, and homeostasis. Despite similarity in recognizing the RGD motif and…
View article: De novo design of highly selective miniprotein inhibitors of integrins αvβ6 and αvβ8
De novo design of highly selective miniprotein inhibitors of integrins αvβ6 and αvβ8 Open
View article: Structural insights into MIC2 recognition by MIC2-associated protein in Toxoplasma gondii
Structural insights into MIC2 recognition by MIC2-associated protein in Toxoplasma gondii Open
View article: Conformational change of <i>Plasmodium</i> <scp>TRAP</scp> is essential for sporozoite migration and transmission
Conformational change of <i>Plasmodium</i> <span>TRAP</span> is essential for sporozoite migration and transmission Open
View article: <i>De novo</i>design of highly selective miniprotein inhibitors of integrins αvβ6 and αvβ8
<i>De novo</i>design of highly selective miniprotein inhibitors of integrins αvβ6 and αvβ8 Open
The RGD (Arg-Gly-Asp)-binding integrins αvβ6 and αvβ8 are clinically validated cancer and fibrosis targets of considerable therapeutic importance. Compounds that can discriminate between the two closely related integrin proteins and other …
View article: A recombinant technique for mapping functional sites of heterotrimeric collagen helices: Collagen IV CB3 fragment as a prototype for integrin binding
A recombinant technique for mapping functional sites of heterotrimeric collagen helices: Collagen IV CB3 fragment as a prototype for integrin binding Open
View article: A specialized integrin-binding motif enables proTGF-β2 activation by integrin αVβ6 but not αVβ8
A specialized integrin-binding motif enables proTGF-β2 activation by integrin αVβ6 but not αVβ8 Open
Activation of latent transforming growth factor (TGF)-β2 is incompletely understood. Unlike TGF-β1 and β3, the TGF-β2 prodomain lacks a seven-residue RGDLXX (L/I) integrin-recognition motif and is thought not to be activated by integrins. …
View article: Structural insights into MIC2 recognition by MIC2-associated protein
Structural insights into MIC2 recognition by MIC2-associated protein Open
Microneme protein 2 (MIC2) and MIC2-associated protein (M2AP) play crucial roles in the gliding motility and host cell invasion of Toxoplasma gondii. Complex formation between MIC2 and M2AP is required for maturation and transport from the…
View article: Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy Open
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
View article: Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy Open
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
View article: Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy Open
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
View article: Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy Open
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
View article: Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy Open
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
View article: Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy Open
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
View article: Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy Open
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
View article: Data from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
Data from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy Open
TGFβ has multiple roles and gene products (TGFβ1, -β2, and -β3), which make global targeting of TGFβ undesirable. Expression of TGFβ requires association with milieu molecules, which localize TGFβ to the surface of specific cells or extrac…
View article: Data from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
Data from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy Open
TGFβ has multiple roles and gene products (TGFβ1, -β2, and -β3), which make global targeting of TGFβ undesirable. Expression of TGFβ requires association with milieu molecules, which localize TGFβ to the surface of specific cells or extrac…
View article: Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy Open
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
View article: Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy Open
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
View article: Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy Open
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
View article: Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy Open
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
View article: Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy Open
Supplementary Figure from Loss of LRRC33-Dependent TGFβ1 Activation Enhances Antitumor Immunity and Checkpoint Blockade Therapy
View article: Alphafold prediction of the Plasmodium TRAP I domain for mutants design.
Alphafold prediction of the Plasmodium TRAP I domain for mutants design. Open
Plasmodium sporozoites have several surface proteins, including the single-pass transmembrane adhesin thrombospondin related anonymous protein (TRAP). TRAP harbors two extracellular domains, the I-domain and the thrombospondin type I repea…