Explanipedia

Coordinated strategy for a model-based decision support tool for coronavirus disease, Utah, USA Open

Hannah R. Meredith, Emerson Arehart, Kyra H. Grantz, Alexander B. Beams, Timothy P. Sheets , et al. · 2024

The coronavirus disease pandemic has highlighted the key role epidemiologic models play in supporting public health decision-making. In particular, these models provide estimates of outbreak potential when data are scarce and decision-maki…

Uncovering receptor-ligand interactions using a high-avidity CRISPR activation screening platform Open

Liping Yang, Timothy P. Sheets, Yang Feng, Guojun Yu, Pradip Bajgain , et al. · 2024

The majority of clinically approved drugs target proteins that are secreted or cell surface bound. However, further advances in this area have been hindered by the challenging nature of receptor deorphanization, as there are still many sec…

dbGuide: a database of functionally validated guide RNAs for genome editing in human and mouse cells Open

Alexander A Gooden, Christine N. Evans, Timothy P. Sheets, Michelle E. Clapp, Raj Chari · 2020

With the technology's accessibility and ease of use, CRISPR has been employed widely in many different organisms and experimental settings. As a result, thousands of publications have used CRISPR to make specific genetic perturbations, est…

dbGuide: A database of functionally validated guide RNAs for genome editing in human and mouse cells Open

Alexander A Gooden, Christine N. Evans, Timothy P. Sheets, Michelle E. Clapp, Raj Chari · 2020

With the technology’s accessibility and ease of use, CRISPR has been employed widely in many different organisms and experimental settings. As a result, thousands of publications have used CRISPR to make specific genetic perturbations, est…

MODULATING KEY GENES INVOLVED IN PANCREAS FORMATION AND INSULIN SIGNALING USING CRISPR/CAS9 IN THE PIG Open

Timothy P. Sheets · 2019

Among the metabolic diseases, diabetes remains a “pressing problem” as recognized by World Health Organization, not only due to the impact on individuals’ lives, but also because of the rapid increase in newly diagnosed patients. To better…

Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs Open

Timothy P. Sheets, Ki‐Eun Park, Chi‐Hun Park, Steven M. Swift, Anne M. Powell , et al. · 2018

Somatic Cell Nuclear Transfer Followed by CRIPSR/Cas9 Microinjection Results in Highly Efficient Genome Editing in Cloned Pigs Open

Timothy P. Sheets, Chi‐Hun Park, Ki‐Eun Park, Anne M. Powell, David M. Donovan , et al. · 2016

The domestic pig is an ideal “dual purpose” animal model for agricultural and biomedical research. With the availability of genome editing tools such as clustered regularly interspaced short palindromic repeat (CRISPR) and associated nucle…

Coexpression of neurogenin 3 (NGN3) and CD133 in cultured human exocrine tissue. Open

Danielle L. Gomez, Marci O’Driscoll, Timothy P. Sheets, Ralph H. Hruban, José Oberholzer , et al. · 2015

A-D, Expression of NGN3 and CD133 in exocrine tissue. A, CD133 expression. B, NGN3 expression. C, Nuclei costained with 4',6-diamidino-2-phenylindole (DAPI). D, Overlay of 3 channels. 1-μm confocal sectio…

Relative mRNA expression of pancreas development transcription factors by CD133+ cells isolated after 4 days of exocrine tissue culture. Open

Danielle L. Gomez, Marci O’Driscoll, Timothy P. Sheets, Ralph H. Hruban, José Oberholzer , et al. · 2015

Mean ± SEM relative expression level of genes in CD133+ cells compared to the CD133-depleted (CD133D) population shown on Y-axis as fold difference. Significance determined by Student’s t-test from 2^-Δct values. ***, p<0.001, **…

Expression of hormones, chromogranin A and PDX1 by CD133+ cells following <i>in vitro</i> differentiation. Open

Danielle L. Gomez, Marci O’Driscoll, Timothy P. Sheets, Ralph H. Hruban, José Oberholzer , et al. · 2015

A, Phase microscopic image of pancospheres on day 6 of formation. Scale bar is 100 μm. B, Pancreatic and duodenal homeobox 1 (PDX1) expression in a day 6 pancosphere. Scale bar is 50 μm. C,D, Orthogonal analyses of PDX…

Engineering large animal models of human disease Open

Bruce Whitelaw, Timothy P. Sheets, Simon Lillico, Bhanu P. Telugu · 2015

The recent development of gene editing tools and methodology for use in livestock enables the production of new animal disease models. These tools facilitate site‐specific mutation of the genome, allowing animals carrying known human disea…

Neurogenin 3 Expressing Cells in the Human Exocrine Pancreas Have the Capacity for Endocrine Cell Fate Open

Danielle L. Gomez, Marci O’Driscoll, Timothy P. Sheets, Ralph H. Hruban, José Oberholzer , et al. · 2015

Neurogenin 3 (NGN3) is necessary and sufficient for endocrine differentiation during pancreatic development and is expressed by a population of progenitor cells that give rise exclusively to hormone-secreting cells within islets. NGN3 prot…

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