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View article: Unprecedented Diversity of the Glycoside Hydrolase Family 70: A Comprehensive Analysis of Sequence, Structure, and Function
Unprecedented Diversity of the Glycoside Hydrolase Family 70: A Comprehensive Analysis of Sequence, Structure, and Function Open
The glycoside hydrolase family 70 (GH70) contains bacterial extracellular multidomain enzymes, synthesizing α-glucans from sucrose or starch-like substrates. A few dozen have been biochemically characterized, while crystal structures cover…
View article: Polysaccharide utilization loci encoded <scp>DUF1735</scp> likely functions as membrane‐bound spacer for carbohydrate active enzymes
Polysaccharide utilization loci encoded <span>DUF1735</span> likely functions as membrane‐bound spacer for carbohydrate active enzymes Open
Proteins featuring the Domain of Unknown Function 1735 are frequently found in Polysaccharide Utilization Loci, yet their role remains unknown. The domain and vicinity analyzer programs we developed mine the Kyoto Encyclopedia of Genes and…
View article: Tailor-Made α-Glucans by Engineering the Processivity of α-Glucanotransferases via Tunnel-Cleft Active Center Interconversions
Tailor-Made α-Glucans by Engineering the Processivity of α-Glucanotransferases via Tunnel-Cleft Active Center Interconversions Open
The function of polysaccharides is intimately associated with their size, which is largely determined by the processivity of transferases responsible for their synthesis. A tunnel active center architecture has been recognized as a key fac…
View article: Insights into the Structure–Function Relationship of GH70 GtfB α-Glucanotransferases from the Crystal Structure and Molecular Dynamic Simulation of a Newly Characterized <i>Limosilactobacillus reuteri</i> N1 GtfB Enzyme
Insights into the Structure–Function Relationship of GH70 GtfB α-Glucanotransferases from the Crystal Structure and Molecular Dynamic Simulation of a Newly Characterized <i>Limosilactobacillus reuteri</i> N1 GtfB Enzyme Open
α-Glucanotransferases of the CAZy family GH70 convert starch-derived donors to industrially important α-glucans. Here, we describe characteristics of a novel GtfB-type 4,6-α-glucanotransferase of high enzyme activity (60.8 U mg-1
View article: Novel β-galactosidase activity and first crystal structure of Glycoside Hydrolase family 154
Novel β-galactosidase activity and first crystal structure of Glycoside Hydrolase family 154 Open
Polysaccharide Utilization Loci (PULs) are physically linked gene clusters conserved in the Gram-negative phylum of Bacteroidota and are valuable sources for Carbohydrate Active enZyme (CAZyme) discovery. This study focuses on BD-β-Gal, an…
View article: Structural and time‐resolved mechanistic investigations of protein hydrolysis by the acidic proline‐specific endoprotease from <i>Aspergillus niger</i>
Structural and time‐resolved mechanistic investigations of protein hydrolysis by the acidic proline‐specific endoprotease from <i>Aspergillus niger</i> Open
Proline‐specific endoproteases have been successfully used in, for example, the in‐situ degradation of gluten, the hydrolysis of bitter peptides, the reduction of haze during beer production, and the generation of peptides for mass spectro…
View article: Crystal Structure of 4,6-α-Glucanotransferase GtfC-ΔC from Thermophilic <i>Geobacillus</i> 12AMOR1: Starch Transglycosylation in Non-Permuted GH70 Enzymes
Crystal Structure of 4,6-α-Glucanotransferase GtfC-ΔC from Thermophilic <i>Geobacillus</i> 12AMOR1: Starch Transglycosylation in Non-Permuted GH70 Enzymes Open
GtfC-type 4,6-α-glucanotransferase (α-GT) enzymes from Glycoside Hydrolase Family 70 (GH70) are of interest for the modification of starch into low-glycemic index food ingredients. Compared to the related GH70 GtfB-type α-GTs, found exclus…
View article: Characterization of the (Engineered) Branching Sucrase GtfZ-CD2 from Apilactobacillus kunkeei for Efficient Glucosylation of Benzenediol Compounds
Characterization of the (Engineered) Branching Sucrase GtfZ-CD2 from Apilactobacillus kunkeei for Efficient Glucosylation of Benzenediol Compounds Open
Glycosylation represents one of the most important ways to expand the diversity of natural products and improve their physico-chemical properties. Aromatic polyphenol compounds widely found in plants are reported to exhibit various remarka…
View article: Crystal Structure of Levansucrase from the Gram-Negative Bacterium <i>Brenneria</i> Provides Insights into Its Product Size Specificity
Crystal Structure of Levansucrase from the Gram-Negative Bacterium <i>Brenneria</i> Provides Insights into Its Product Size Specificity Open
Microbial levansucrases (LSs, EC 2.4.1.10) have been widely studied for the synthesis of β-(2,6)-fructans (levan) from sucrose. LSs synthesize levan-type fructo-oligosaccharides, high-molecular-mass levan polymer or combinations of both. H…
View article: Mutations in Amino Acid Residues of <i>Limosilactobacillus reuteri</i> 121 GtfB 4,6-α-Glucanotransferase that Affect Reaction and Product Specificity
Mutations in Amino Acid Residues of <i>Limosilactobacillus reuteri</i> 121 GtfB 4,6-α-Glucanotransferase that Affect Reaction and Product Specificity Open
Limosilactobacillus reuteri 121 4,6-α-glucanotransferase (Lr121 4,6-α-GTase), belonging to the glycosyl hydrolase (GH) 70 GtfB subfamily, converts starch and maltodextrins into linear isomalto/malto polysaccharides (IMMPs) with cons…
View article: Enzymatic glucosylation of polyphenols using glucansucrases and branching sucrases of glycoside hydrolase family 70
Enzymatic glucosylation of polyphenols using glucansucrases and branching sucrases of glycoside hydrolase family 70 Open
Polyphenols exhibit various beneficial biological activities and represent very promising candidates as active compounds for food industry. However, the low solubility, poor stability and low bioavailability of polyphenols have severely li…
View article: Insights into Broad-Specificity Starch Modification from the Crystal Structure of <i>Limosilactobacillus Reuteri</i> NCC 2613 4,6-α-Glucanotransferase GtfB
Insights into Broad-Specificity Starch Modification from the Crystal Structure of <i>Limosilactobacillus Reuteri</i> NCC 2613 4,6-α-Glucanotransferase GtfB Open
GtfB-type α-glucanotransferase enzymes from glycoside hydrolase family 70 (GH70) convert starch substrates into α-glucans that are of interest as food ingredients with a low glycemic index. Characterization of several GtfBs showed that the…
View article: Crystal structure of an inulosucrase from <i>Halalkalicoccus</i> <i>jeotgali</i> B3T, a halophilic archaeal strain
Crystal structure of an inulosucrase from <i>Halalkalicoccus</i> <i>jeotgali</i> B3T, a halophilic archaeal strain Open
Several archaea harbor genes that code for fructosyltransferase (FTF) enzymes. These enzymes have not been characterized yet at structure–function level, but are of extreme interest in view of their potential role in the synthesis of novel…
View article: Author response for "Crystal structure of an inulosucrase from Halalkalicoccus jeotgali B3T‐a halophilic archaeal strain"
Author response for "Crystal structure of an inulosucrase from Halalkalicoccus jeotgali B3T‐a halophilic archaeal strain" Open
View article: Using Mutability Landscapes To Guide Enzyme Thermostabilization
Using Mutability Landscapes To Guide Enzyme Thermostabilization Open
Thermostabilizing enzymes while retaining their activity and enantioselectivity for applied biocatalysis is an important topic in protein engineering. Rational and computational design strategies as well as directed evolution have been use…
View article: Identification of Thermotoga maritima MSB8 GH57 α-amylase AmyC as a glycogen-branching enzyme with high hydrolytic activity
Identification of Thermotoga maritima MSB8 GH57 α-amylase AmyC as a glycogen-branching enzyme with high hydrolytic activity Open
View article: Enantioselective Synthesis of Pharmaceutically Active γ-Aminobutyric Acids Using a Tailor-Made Artificial Michaelase in One-Pot Cascade Reactions
Enantioselective Synthesis of Pharmaceutically Active γ-Aminobutyric Acids Using a Tailor-Made Artificial Michaelase in One-Pot Cascade Reactions Open
Chiral γ-aminobutyric acid (GABA) analogues represent abundantly prescribed drugs, which are broadly applied as anticonvulsants, as antidepressants, and for the treatment of neuropathic pain. Here we report a one-pot two-step biocatalytic …
View article: Mutational Analysis of the Role of the Glucansucrase Gtf180-ΔN Active Site Residues in Product and Linkage Specificity with Lactose as Acceptor Substrate
Mutational Analysis of the Role of the Glucansucrase Gtf180-ΔN Active Site Residues in Product and Linkage Specificity with Lactose as Acceptor Substrate Open
Glucansucrase Gtf180-ΔN from Lactobacillus reuteri uses lactose as acceptor substrate to synthesize five glucosylated lactose molecules (F1-F5) with a degree of polymerization (DP) of 3-4 (GL34) and with (α1→2)/(α1→3)/(α1→4) glycosidic lin…
View article: Biochemical characterization of a GH70 protein from Lactobacillus kunkeei DSM 12361 with two catalytic domains involving branching sucrase activity
Biochemical characterization of a GH70 protein from Lactobacillus kunkeei DSM 12361 with two catalytic domains involving branching sucrase activity Open
View article: Biochemical and genetic characterization of fungal proline hydroxylase in echinocandin biosynthesis
Biochemical and genetic characterization of fungal proline hydroxylase in echinocandin biosynthesis Open
View article: Biochemical characterization of two GH70 family 4,6-α-glucanotransferases with distinct product specificity from Lactobacillus aviarius subsp. aviarius DSM 20655
Biochemical characterization of two GH70 family 4,6-α-glucanotransferases with distinct product specificity from Lactobacillus aviarius subsp. aviarius DSM 20655 Open
Nine GtfB-like 4,6-α-glucanotransferases (4,6-α-GTs) (represented by GtfX of L. aviarius subsp. aviarius DSM 20655) were identified to show distinct characteristics in conserved motifs I-IV. In particular, the "fingerprint" Tyr in motif II…
View article: Biotechnological potential of novel glycoside hydrolase family 70 enzymes synthesizing α-glucans from starch and sucrose
Biotechnological potential of novel glycoside hydrolase family 70 enzymes synthesizing α-glucans from starch and sucrose Open
View article: A general mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy
A general mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy Open
View article: Mining novel starch-converting Glycoside Hydrolase 70 enzymes from the Nestlé Culture Collection genome database: The Lactobacillus reuteri NCC 2613 GtfB
Mining novel starch-converting Glycoside Hydrolase 70 enzymes from the Nestlé Culture Collection genome database: The Lactobacillus reuteri NCC 2613 GtfB Open
View article: Biochemical Characterization of the Functional Roles of Residues in the Active Site of the β-Galactosidase from <i>Bacillus circulans</i> ATCC 31382
Biochemical Characterization of the Functional Roles of Residues in the Active Site of the β-Galactosidase from <i>Bacillus circulans</i> ATCC 31382 Open
The β-galactosidase enzyme from Bacillus circulans ATCC 31382 BgaD is widely used in the food industry to produce prebiotic galactooligosaccharides (GOS). Recently, the crystal structure of a C-terminally truncated version of the enzyme (B…
View article: A new group of glycoside hydrolase family 13 α-amylases with an aberrant catalytic triad
A new group of glycoside hydrolase family 13 α-amylases with an aberrant catalytic triad Open
View article: Engineering of the <i>Bacillus circulans</i> β-Galactosidase Product Specificity
Engineering of the <i>Bacillus circulans</i> β-Galactosidase Product Specificity Open
Microbial β-galactosidase enzymes are widely used as biocatalysts in industry to produce prebiotic galactooligosaccharides (GOS) from lactose. GOS mixtures are used as beneficial additives in infant formula to mimic the prebiotic effects o…
View article: Crystal Structure of 4,6-α-Glucanotransferase Supports Diet-Driven Evolution of GH70 Enzymes from α-Amylases in Oral Bacteria
Crystal Structure of 4,6-α-Glucanotransferase Supports Diet-Driven Evolution of GH70 Enzymes from α-Amylases in Oral Bacteria Open
View article: 4,3-α-Glucanotransferase, a novel reaction specificity in glycoside hydrolase family 70 and clan GH-H
4,3-α-Glucanotransferase, a novel reaction specificity in glycoside hydrolase family 70 and clan GH-H Open
View article: Structural determinants of alternating (α1 → 4) and (α1 → 6) linkage specificity in reuteransucrase of Lactobacillus reuteri
Structural determinants of alternating (α1 → 4) and (α1 → 6) linkage specificity in reuteransucrase of Lactobacillus reuteri Open