Tobias Baumgart
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View article: Endophilin–lamellipodin–VASP, key components in fast endophilin–mediated endocytosis, control actin polymerization within liquid-like condensates
Endophilin–lamellipodin–VASP, key components in fast endophilin–mediated endocytosis, control actin polymerization within liquid-like condensates Open
Actin polymerization is essential in several clathrin-independent endocytic pathways including fast endophilin-mediated endocytosis (FEME); however, the actin machinery involved in FEME has been elusive. Here, we show that the actin polyme…
View article: Genetic code expansion and enzymatic modifications as accessible methods for studying site‐specific post‐translational modifications of alpha‐synuclein and tau
Genetic code expansion and enzymatic modifications as accessible methods for studying site‐specific post‐translational modifications of alpha‐synuclein and tau Open
Alpha‐synuclein (αS) and tau play important roles in the pathology of Parkinson's disease and Alzheimer's disease, respectively, as well as numerous other neurodegenerative diseases. Both proteins are classified as intrinsically disordered…
View article: Genetic Code Expansion and Enzymatic Modifications as Accessible Methods for Studying Site-Specific Post-Translational Modifications of Alpha-Synuclein and Tau
Genetic Code Expansion and Enzymatic Modifications as Accessible Methods for Studying Site-Specific Post-Translational Modifications of Alpha-Synuclein and Tau Open
Alpha-synuclein (αS) and tau play important roles in the pathology of Parkinson’s Disease and Alzheimer’s Disease, respectively, as well as numerous other neurodegenerative diseases. Both proteins are classified as intrinsically disordered…
View article: Strategic Modulation of Polarity and Viscosity Sensitivity of Bimane Molecular Rotor-Based Fluorophores for Imaging α-Synuclein
Strategic Modulation of Polarity and Viscosity Sensitivity of Bimane Molecular Rotor-Based Fluorophores for Imaging α-Synuclein Open
Molecular rotor-based fluorophores (RBFs) that are target-selective and sensitive to both polarity and viscosity are valuable for diverse biological applications. Here, we have designed next-generation RBFs based on the underexplored biman…
View article: Strategic Modulation of Polarity and Viscosity Sensitivity of Bimane Molecular Rotor-Based Fluorophores for Imaging α-Synuclein
Strategic Modulation of Polarity and Viscosity Sensitivity of Bimane Molecular Rotor-Based Fluorophores for Imaging α-Synuclein Open
Molecular rotor-based fluorophores (RBFs) that are target-selective and sensitive to both polarity and viscosity are valuable for diverse biological applications. Here, we have designed next-generation RBFs based on the underexplored biman…
View article: Asymmetric crowders and membrane morphology at the nexus of intracellular trafficking and oncology
Asymmetric crowders and membrane morphology at the nexus of intracellular trafficking and oncology Open
A definitive understanding of the interplay between protein binding/migration and membrane curvature evolution is emerging but needs further study. The mechanisms defining such phenomena are critical to intracellular transport and traffick…
View article: Endophilin-Lamellipodin-VASP, key components in fast endophilin-mediated endocytosis, control actin polymerization within liquid-like condensates
Endophilin-Lamellipodin-VASP, key components in fast endophilin-mediated endocytosis, control actin polymerization within liquid-like condensates Open
Actin polymerization is essential in several clathrin-independent endocytic pathways including fast endophilin mediated endocytosis (FEME), however the actin machinery involved in FEME has been elusive. Here, we show that the actin polymer…
View article: Methyl-β-cyclodextrin asymmetrically extracts phospholipid from bilayers, granting tunable control over differential stress in lipid vesicles
Methyl-β-cyclodextrin asymmetrically extracts phospholipid from bilayers, granting tunable control over differential stress in lipid vesicles Open
In this study, we show that complexation between methyl-β-cyclodextrin and phospholipids can be leveraged to control the lipid asymmetry of synthetic vesicles. Additionally, we propose a modified equilibrium model for mbCD–lipid complexati…
View article: Purification of Recombinant Human Amphiphysin 1 and its N-BAR Domain
Purification of Recombinant Human Amphiphysin 1 and its N-BAR Domain Open
Bin/Amphiphysin/Rvs (BAR) proteins are known as classical membrane curvature generators during endocytosis. Amphiphysin, a member of the N-BAR sub-family of proteins that contain a characteristic amphipathic sequence at the N-terminus of t…
View article: Probing lipid membrane bending mechanics using gold nanorod tracking
Probing lipid membrane bending mechanics using gold nanorod tracking Open
Lipid bilayer membranes undergo rapid bending undulations with wavelengths from tens of nanometers to tens of microns due to thermal fluctuations. Here, we probe such undulations and the membranes' mechanics by measuring the time-varying o…
View article: Multivalent Interactions between Molecular Components Involved in Fast Endophilin Mediated Endocytosis Drive Protein Phase Separation
Multivalent Interactions between Molecular Components Involved in Fast Endophilin Mediated Endocytosis Drive Protein Phase Separation Open
A specific group of transmembrane receptors, including the β1-adrenergic receptor (β1-AR), is internalized through a non-clathrin pathway known as Fast Endophilin Mediated Endocytosis (FEME). A key question is: how does the endocytic machi…
View article: Unfolding Mechanisms and Conformational Stability of the Dimeric Endophilin N-BAR Domain
Unfolding Mechanisms and Conformational Stability of the Dimeric Endophilin N-BAR Domain Open
Endophilin, which is a member of the Bin-amphiphysin-Rvs (BAR) domain protein superfamily, contains a homodimeric N-BAR domain of a characteristic crescent shape. The N-BAR domain comprises a six-helix bundle and is known to sense and gene…
View article: A needless but interesting controversy
A needless but interesting controversy Open
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View article: Probing lipid membrane bending mechanics using gold nanorod tracking
Probing lipid membrane bending mechanics using gold nanorod tracking Open
Lipid bilayer membranes undergo rapid bending undulations with wavelengths from tens of nanometers to tens of microns due to thermal fluctuations. Here, we probe such undulations and the membranes' mechanics by measuring the time-varying o…
View article: Interactions between Phase-Separated Liquids and Membrane Surfaces
Interactions between Phase-Separated Liquids and Membrane Surfaces Open
Liquid-liquid phase separation has recently emerged as an important fundamental organizational phenomenon in biological settings. Most studies of biological phase separation have focused on droplets that “condense” from solution above a cr…
View article: Asymmetric desorption of lipid oxidation products induces membrane bending
Asymmetric desorption of lipid oxidation products induces membrane bending Open
Binding, unbinding, and flipping kinetics of lipid oxidation products can be investigated using a microfluidic approach with micropipette aspiration of giant unilamellar vesicles.
View article: Endophilin recruitment drives membrane curvature generation through coincidence detection of GPCR loop interactions and negative lipid charge
Endophilin recruitment drives membrane curvature generation through coincidence detection of GPCR loop interactions and negative lipid charge Open
Endophilin plays key roles during endocytosis of cellular receptors, including generating membrane curvature to drive internalization. Electrostatic interactions between endophilin's BIN/Amphiphysin/Rvs domain and anionic membrane lipids h…