Tobias Gräwert
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View article: AF4-to-SAXS: expanded characterization of nanoparticles and proteins at the P12 BioSAXS beamline
AF4-to-SAXS: expanded characterization of nanoparticles and proteins at the P12 BioSAXS beamline Open
Biological small-angle X-ray scattering (SAXS) is a versatile and powerful technique for investigating the structural and biophysical properties of biologically and pharmaceutically relevant macromolecules and nanoparticles. SAXS offers de…
View article: New features in <i>ATSAS-4</i> , a program suite for small-angle scattering data analysis
New features in <i>ATSAS-4</i> , a program suite for small-angle scattering data analysis Open
The ATSAS release 4 series introduces substantial architectural updates and enhanced user-friendly tools for analyzing biological macromolecules and nanostructured objects. In particular, highly requested project support for graphical user…
View article: Structural basis of CDNF interaction with the UPR regulator GRP78
Structural basis of CDNF interaction with the UPR regulator GRP78 Open
View article: Small-angle x-ray scattering investigation of the integration of free fatty acids in polysorbate 20 micelles
Small-angle x-ray scattering investigation of the integration of free fatty acids in polysorbate 20 micelles Open
View article: Small-angle x-ray scattering investigation of the integration of free fatty acids in polysorbate 20 micelles
Small-angle x-ray scattering investigation of the integration of free fatty acids in polysorbate 20 micelles Open
View article: Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2
Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2 Open
View article: Mechanism of activation and regulation of Deubiquitinase activity in MINDY1 and MINDY2
Mechanism of activation and regulation of Deubiquitinase activity in MINDY1 and MINDY2 Open
Of the eight distinct polyubiquitin chains that can be assembled, K48-linked ubiquitin is the most well-understood linkage and modification of proteins with K48 chains targets the modified protein for degradation. By removing ubiquitin fro…
View article: Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Open
We describe the updated size-exclusion chromatography small angle X-ray scattering (SEC-SAXS) set-up used at the P12 bioSAXS beam line of the European Molecular Biology Laboratory (EMBL) at the PETRAIII synchrotron, DESY Hamburg (Germany).…
View article: Structural Kinetics of MsbA Investigated by Stopped-Flow Time-Resolved Small-Angle X-Ray Scattering
Structural Kinetics of MsbA Investigated by Stopped-Flow Time-Resolved Small-Angle X-Ray Scattering Open
View article: Structure of GTP cyclohydrolase I from <i>Listeria monocytogenes</i> , a potential anti-infective drug target
Structure of GTP cyclohydrolase I from <i>Listeria monocytogenes</i> , a potential anti-infective drug target Open
A putative open reading frame encoding GTP cyclohydrolase I from Listeria monocytogenes was expressed in a recombinant Escherichia coli strain. The recombinant protein was purified and was confirmed to convert GTP to dihydroneopterin triph…
View article: Novel reverse thia-analogs of fosmidomycin: Synthesis and antiplasmodial activity
Novel reverse thia-analogs of fosmidomycin: Synthesis and antiplasmodial activity Open
View article: Aryl bis-sulfonamides bind to the active site of a homotrimeric isoprenoid biosynthesis enzyme IspF and extract the essential divalent metal cation cofactor
Aryl bis-sulfonamides bind to the active site of a homotrimeric isoprenoid biosynthesis enzyme IspF and extract the essential divalent metal cation cofactor Open
Native ESI-MS delivers unprecedented insight into unknown homomeric protein binding mechanisms involving complex, multistage binding equilibria with cofactors and ligands.
View article: Inhibition of the Non-Mevalonate Isoprenoid Pathway by Reverse Hydroxamate Analogues of Fosmidomycin
Inhibition of the Non-Mevalonate Isoprenoid Pathway by Reverse Hydroxamate Analogues of Fosmidomycin Open
The non-mevalonate (methylerythritol phosphate, MEP) pathway for isoprenoid biosynthesis is essential in Plasmodium spp.., but is absent in the human host. The pathway is a clinically validated antimalarial target on basis of studies with …