Tobias Weinert
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View article: Time-resolved structures of β<sub>2</sub>-adrenergic receptor modulation by a photoswitchable beta-blocker
Time-resolved structures of β<sub>2</sub>-adrenergic receptor modulation by a photoswitchable beta-blocker Open
G protein-coupled receptors (GPCRs) regulate essential physiological responses and are important drug targets, yet their ligand-induced conformational dynamics remain poorly understood. The β 2 -adrenergic receptor (β 2 AR) is a prominent …
View article: Capturing macromolecular dynamics using time-resolved serial crystallography at X- ray free electron lasers and synchrotron light sources
Capturing macromolecular dynamics using time-resolved serial crystallography at X- ray free electron lasers and synchrotron light sources Open
In recent years, time-resolved serial crystallography has emerged as a transformative technique for unraveling the intricate dynamics of macromolecules at atomic resolution. By leveraging the high-intensity and ultra-short pulses of X-ray …
View article: Structural basis for the prolonged photocycle of sensory rhodopsin II revealed by serial synchrotron crystallography
Structural basis for the prolonged photocycle of sensory rhodopsin II revealed by serial synchrotron crystallography Open
Microbial rhodopsins form a diverse family of light-sensitive seven-transmembrane helix retinal proteins that function as active proton or ion pumps, passive light-gated ion channels, and photosensors. To understand how light-sensing in ar…
View article: Photoswitch dissociation from a G protein-coupled receptor resolved by time-resolved serial crystallography
Photoswitch dissociation from a G protein-coupled receptor resolved by time-resolved serial crystallography Open
View article: Structural Insights Into the Opening Mechanism of C1C2 Channelrhodopsin
Structural Insights Into the Opening Mechanism of C1C2 Channelrhodopsin Open
Channelrhodopsins, light-gated cation channels, enable precise control of neural cell depolarization or hyperpolarization with light in the field of optogenetics. This study integrates time-resolved serial crystallography and atomistic mol…
View article: Structural effects of high laser power densities on an early bacteriorhodopsin photocycle intermediate
Structural effects of high laser power densities on an early bacteriorhodopsin photocycle intermediate Open
Time-resolved serial crystallography at X-ray Free Electron Lasers offers the opportunity to observe ultrafast photochemical reactions at the atomic level. The technique has yielded exciting molecular insights into various biological proce…
View article: Structural insights into the opening mechanism of C1C2 channelrhodopsin
Structural insights into the opening mechanism of C1C2 channelrhodopsin Open
Channelrhodopsins, light-gated cation channels, enable precise control of neural cell depolarization or hyperpolarization with light in the field of optogenetics. This study integrates time-resolved serial crystallography and atomistic mol…
View article: Capturing the blue-light activated state of the Phot-LOV1 domain from <i>Chlamydomonas reinhardtii</i> using time-resolved serial synchrotron crystallography
Capturing the blue-light activated state of the Phot-LOV1 domain from <i>Chlamydomonas reinhardtii</i> using time-resolved serial synchrotron crystallography Open
Light–oxygen–voltage (LOV) domains are small photosensory flavoprotein modules that allow the conversion of external stimuli (sunlight) into intracellular signals responsible for various cell behaviors ( e.g. phototropism and chloroplast r…
View article: Photochemical affinity switches to resolve ligand dissociation from a G protein-coupled receptor by time-resolved serial crystallography
Photochemical affinity switches to resolve ligand dissociation from a G protein-coupled receptor by time-resolved serial crystallography Open
G protein-coupled receptors (GPCRs) are the largest family of cell surface receptors in humans. The binding and dissociation of ligands tunes the inherent conformational flexibility of these important drug targets towards distinct function…
View article: The time revolution in macromolecular crystallography
The time revolution in macromolecular crystallography Open
Macromolecular crystallography has historically provided the atomic structures of proteins fundamental to cellular functions. However, the advent of cryo-electron microscopy for structure determination of large and increasingly smaller and…
View article: Directed ultrafast conformational changes accompany electron transfer in a photolyase as resolved by serial crystallography
Directed ultrafast conformational changes accompany electron transfer in a photolyase as resolved by serial crystallography Open
Charge-transfer reactions in proteins are important for life, such as in photolyases which repair DNA, but the role of structural dynamics remains unclear. Here, using femtosecond X-ray crystallography, we report the structural changes tha…
View article: Direct observation of coherent azobenzene photochemistry
Direct observation of coherent azobenzene photochemistry Open
Molecular photoswitches are versatile natural or synthetic molecules that undergo reversible conformational changes in response to light. In chemistry azobenzenes act as ubiquitous synthetic photoswitches1 with applications rang…
View article: A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers
A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers Open
Serial crystallography at X-ray free-electron lasers (XFELs) permits the determination of radiation-damage free static as well as time-resolved protein structures at room temperature. Efficient sample delivery is a key factor for such expe…
View article: Visualizing the DNA repair process by a photolyase at atomic resolution
Visualizing the DNA repair process by a photolyase at atomic resolution Open
Photolyases, a ubiquitous class of flavoproteins, use blue light to repair DNA photolesions. In this work, we determined the structural mechanism of the photolyase-catalyzed repair of a cyclobutane pyrimidine dimer (CPD) lesion using time-…
View article: Capturing the blue-light activated state of the Phot-LOV1 domain from<i>Chlamydomonas reinhardtii</i>using time-resolved serial synchrotron crystallography
Capturing the blue-light activated state of the Phot-LOV1 domain from<i>Chlamydomonas reinhardtii</i>using time-resolved serial synchrotron crystallography Open
Light-Oxygen-Voltage (LOV) domains are small photosensory flavoprotein modules that allow converting external stimuli (sunlight) into intracellular signals responsible for various cell behavior ( e . g., phototropism and chloroplast reloca…
View article: Kilohertz serial crystallography with the JUNGFRAU detector at a fourth-generation synchrotron source
Kilohertz serial crystallography with the JUNGFRAU detector at a fourth-generation synchrotron source Open
Serial and time-resolved macromolecular crystallography are on the rise. However, beam time at X-ray free-electron lasers is limited and most third-generation synchrotron-based macromolecular crystallography beamlines do not offer the nece…
View article: Low-pass spectral analysis of time-resolved serial femtosecond crystallography data
Low-pass spectral analysis of time-resolved serial femtosecond crystallography data Open
Low-pass spectral analysis (LPSA) is a recently developed dynamics retrieval algorithm showing excellent retrieval properties when applied to model data affected by extreme incompleteness and stochastic weighting. In this work, we apply LP…
View article: Ultrafast structural changes direct the first molecular events of vision
Ultrafast structural changes direct the first molecular events of vision Open
View article: A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers
A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers Open
Serial crystallography at X-ray free-electron lasers (XFELs) permits the determination of radiation-damage free static as well as time-resolved protein structures at room temperature. Efficient sample delivery is a key factor for such expe…
View article: Correction of rhodopsin serial crystallography diffraction intensities for a lattice-translocation defect
Correction of rhodopsin serial crystallography diffraction intensities for a lattice-translocation defect Open
Rhodopsin is a G-protein-coupled receptor that detects light and initiates the intracellular signalling cascades that underpin vertebrate vision. Light sensitivity is achieved by covalent linkage to 11- cis retinal, which isomerizes upon p…
View article: Watching the release of a photopharmacological drug from tubulin using time-resolved serial crystallography
Watching the release of a photopharmacological drug from tubulin using time-resolved serial crystallography Open
View article: Femtosecond conformational changes in a photolyase guidelong-range electron transfer as resolved by serial crystallography
Femtosecond conformational changes in a photolyase guidelong-range electron transfer as resolved by serial crystallography Open
Charge transfer reactions in proteins are fundamentally important for life, but it is currently not clear how protein structural dynamics control these electron transfer reactions. Photolyases/cryptochromes, which repair DNA and signal in …
View article: ULTRAFAST STRUCTURAL CHANGES DIRECT THE FIRST MOLECULAR EVENTS OF VISION
ULTRAFAST STRUCTURAL CHANGES DIRECT THE FIRST MOLECULAR EVENTS OF VISION Open
Vision is initiated by the rhodopsin family of light-sensitive G protein-coupled receptors (GPCRs). A photon is absorbed by the 11- cis retinal chromophore of rhodopsin which isomerises within 200 femtoseconds to the all- trans conformatio…
View article: Release of a photopharmacological drug from its protein target captured by time-resolved serial crystallography
Release of a photopharmacological drug from its protein target captured by time-resolved serial crystallography Open
The binding and release of ligands from their protein targets is central to fundamental biological processes as well as to drug discovery. Photopharmacology introduces chemical triggers that allow the changing of ligand affinities and thus…
View article: <i>In Vivo</i> Photocontrol of Microtubule Dynamics and Integrity, Migration and Mitosis, by the Potent GFP-Imaging-Compatible Photoswitchable Reagents SBTubA4P and SBTub2M
<i>In Vivo</i> Photocontrol of Microtubule Dynamics and Integrity, Migration and Mitosis, by the Potent GFP-Imaging-Compatible Photoswitchable Reagents SBTubA4P and SBTub2M Open
Photoswitchable reagents are powerful tools for high-precision studies in cell biology. When these reagents are globally administered yet locally photoactivated in two-dimensional (2D) cell cultures, they can exert micron- and millisecond-…
View article: Dynamics and mechanism of a light-driven chloride pump
Dynamics and mechanism of a light-driven chloride pump Open
Chloride transport by microbial rhodopsins is an essential process for which molecular details such as the mechanisms that convert light energy to drive ion pumping and ensure the unidirectionality of the transport have remained elusive. W…
View article: Watching the release of a photopharmacological drug from tubulin using time-resolved serial crystallography
Watching the release of a photopharmacological drug from tubulin using time-resolved serial crystallography Open
The binding and release of ligands from their protein targets is central to fundamental biological processes as well as to drug discovery. Photopharmacology introduces chemical triggers that allow the changing of ligand affinities and thus…
View article: Structural basis of the radical pair state in photolyases and cryptochromes
Structural basis of the radical pair state in photolyases and cryptochromes Open
The structural response of the drosophila (6-4) photolyase to photoinduced electron transfer along a chain of tryptophans is revealed using a serial crystallographic snapshot of the protein in its radical pair state.
View article: Pink-beam serial femtosecond crystallography for accurate structure-factor determination at an X-ray free-electron laser
Pink-beam serial femtosecond crystallography for accurate structure-factor determination at an X-ray free-electron laser Open
Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables essentially radiation-damage-free macromolecular structure determination using microcrystals that are too small for synchrotron studies. However, SFX ex…
View article: Pink-beam serial femtosecond crystallography for accurate structure factor determination at an X-ray free-electron laser
Pink-beam serial femtosecond crystallography for accurate structure factor determination at an X-ray free-electron laser Open
Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables essentially radiation damage-free macromolecular structure determination using microcrystals that are too small for synchrotron studies [1].However, SFX…