Tom Egger
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View article: TopBP1 biomolecular condensates as a new therapeutic target in advanced-stage colorectal cancer
TopBP1 biomolecular condensates as a new therapeutic target in advanced-stage colorectal cancer Open
In cancer cells, ATR signaling is crucial to tolerate the intrinsically high damage levels that normally block replication fork progression. Assembly of TopBP1, a multifunctional scaffolding protein, into condensates is required to amplify…
View article: Shining light on drug discovery: optogenetic screening for TopBP1 biomolecular condensate inhibitors
Shining light on drug discovery: optogenetic screening for TopBP1 biomolecular condensate inhibitors Open
Human topoisomerase IIβ binding protein 1 (TopBP1) is a scaffold protein involved in DNA replication initiation, DNA repair, transcription regulation, and checkpoint activation. TopBP1 forms nuclear condensates that act as a molecular swit…
View article: TopBP1 biomolecular condensates: a new therapeutic target in advanced-stage colorectal cancer
TopBP1 biomolecular condensates: a new therapeutic target in advanced-stage colorectal cancer Open
In cancer cells, ATR signaling is crucial to tolerate the intrinsically high damage levels that normally block replication fork progression. Assembly of TopBP1, a multifunctional scaffolding protein, into condensates is required to amplify…
View article: KIF2C condensation concentrates PLK1 and phosphorylated BRCA2 on kinetochore microtubules in mitosis
KIF2C condensation concentrates PLK1 and phosphorylated BRCA2 on kinetochore microtubules in mitosis Open
During mitosis, the microtubule depolymerase KIF2C, the tumor suppressor BRCA2, and the kinase PLK1 contribute to the control of kinetochore-microtubule attachments. Both KIF2C and BRCA2 are phosphorylated by PLK1, and BRCA2 phosphorylated…
View article: TopBP1 biomolecular condensates: a new therapeutic target in advanced-stage colorectal cancer
TopBP1 biomolecular condensates: a new therapeutic target in advanced-stage colorectal cancer Open
In cancer cells, ATR signaling is crucial to tolerate the intrinsically high damage levels that normally block replication fork progression. Assembly of TopBP1, a multifunctional scaffolding protein, into condensates is required to amplify…
View article: TopBP1 biomolecular condensates as a new therapeutic target in advanced-stage colorectal cancer
TopBP1 biomolecular condensates as a new therapeutic target in advanced-stage colorectal cancer Open
In cancer cells, ATR signaling is crucial to tolerate the intrinsically high damage levels that normally block replication fork progression. Assembly of TopBP1, a multifunctional scaffolding protein, into condensates is required to amplify…
View article: TopBP1 biomolecular condensates: a new therapeutic target in advanced-stage colorectal cancer
TopBP1 biomolecular condensates: a new therapeutic target in advanced-stage colorectal cancer Open
In cancer cells, ATR signaling is crucial to tolerate the intrinsically high damage levels that normally block replication fork progression. Assembly of TopBP1, a multifunctional scaffolding protein, into condensates is required to amplify…
View article: A non-catalytic function for Rad18 in sustaining glioblastoma proliferation
A non-catalytic function for Rad18 in sustaining glioblastoma proliferation Open
The Rad18 E3 ubiquitin ligase, a non-essential gene, is a key regulator of DNA damage tolerance that also functions in repair of DNA double strand breaks. Rad18 is overexpressed in the aggressive brain cancer glioblastoma (GBM) and its dow…
View article: KIF2C-induced nuclear condensation concentrates PLK1 and phosphorylated BRCA2 at the kinetochore microtubules in mitosis
KIF2C-induced nuclear condensation concentrates PLK1 and phosphorylated BRCA2 at the kinetochore microtubules in mitosis Open
SUMMARY During mitosis, the human microtubule depolymerase KIF2C increases the turnover of kinetochore-microtubule attachments. This facilitates the correction of attachment errors. Moreover, BRCA2 phosphorylated at Thr207 by PLK1 (BRCA2-p…
View article: Spatial organization and functions of Chk1 activation by TopBP1 biomolecular condensates
Spatial organization and functions of Chk1 activation by TopBP1 biomolecular condensates Open
Assembly of TopBP1 biomolecular condensates triggers activation of the ataxia telangiectasia-mutated and Rad3-related (ATR)/Chk1 signaling pathway, which coordinates cell responses to impaired DNA replication. Here, we used optogenetics an…
View article: Protocol to analyze endogenous translesion DNA synthesis in single mammalian cells
Protocol to analyze endogenous translesion DNA synthesis in single mammalian cells Open
Translesion DNA synthesis (TLS) is an evolutionarily conserved branch of the cellular DNA damage tolerance pathway that is often exploited by cancer cells to overcome therapy resistance. Here, we present a protocol to analyze endogenous TL…
View article: Detection of endogenous translesion DNA synthesis in single mammalian cells
Detection of endogenous translesion DNA synthesis in single mammalian cells Open
Translesion DNA synthesis (TLS) is an evolutionarily conserved process that cells activate to tolerate DNA damage. TLS facilitates proliferation under DNA damage conditions and is exploited by cancer cells to gain therapy resistance. It ha…
View article: Dicer prevents genome instability in response to replication stress
Dicer prevents genome instability in response to replication stress Open
Dicer, an endoribonuclease best-known for its role in microRNA biogenesis and RNA interference pathway, has been shown to play a role in the DNA damage response and repair of double-stranded DNA breaks (DSBs) in mammalian cells. However, i…