Tom Henley
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View article: CISH, a novel intracellular immune checkpoint, in comparison and combination to existing and emerging cancer immune checkpoints
CISH, a novel intracellular immune checkpoint, in comparison and combination to existing and emerging cancer immune checkpoints Open
Over the past decade, Immuno-Oncology has largely focused on blocking inhibitory surface receptors like PD-1 to enhance T cell anti-tumor activity. However, intracellular immune checkpoints such as CISH, which function independently of tum…
View article: A Novel CRISPR-Engineered, Stem Cell-Derived Cellular Vaccine
A Novel CRISPR-Engineered, Stem Cell-Derived Cellular Vaccine Open
COVID-19 has forced rapid clinical translation of novel vaccine technologies, principally mRNA vaccines, that have resulted in meaningful efficacy and adequate safety in response to the global pandemic. Notwithstanding this success, there …
View article: Homology mediated end joining enables efficient non-viral targeted integration of large DNA templates in primary human T cells
Homology mediated end joining enables efficient non-viral targeted integration of large DNA templates in primary human T cells Open
Adoptive cellular therapy using genetically engineered immune cells holds tremendous promise for the treatment of advanced cancers. While the number of available receptors targeting tumor specific antigens continues to grow, the current re…
View article: Genetic editing of <i>CISH</i> enhances T cell effector programs independently of immune checkpoint cell surface ligand expression
Genetic editing of <i>CISH</i> enhances T cell effector programs independently of immune checkpoint cell surface ligand expression Open
PD-1 acts as a negative regulator of T cell-mediated immune responses in the setting of persistent antigen expression, including cancer and chronic pathogen infections. Antibody-mediated blockade of the PD-1/PD-L1 axis benefits a subset of…
View article: Improved Genome Packaging Efficiency of Adeno-associated Virus Vectors Using Rep Hybrids
Improved Genome Packaging Efficiency of Adeno-associated Virus Vectors Using Rep Hybrids Open
A major by-product of all adeno-associated virus (AAV) vector production systems are “empty” capsids, void of the desired therapeutic gene, and thus do not provide any curative benefit for the treatment of the targeted disease. In fact, em…
View article: Improved Genome Packaging Efficiency of AAV Vectors Using Rep Hybrids
Improved Genome Packaging Efficiency of AAV Vectors Using Rep Hybrids Open
Recombinant Adeno-associated viruses (rAAVs) are one of the most commonly used vectors for a variety of gene therapy applications. In the last two decades research focused primarily on the characterization and isolation of new cap genes re…
View article: A Bioinformatic and Empiric Exploration of Prokaryotic Argonautes as Novel Programmable Endonuclease Systems
A Bioinformatic and Empiric Exploration of Prokaryotic Argonautes as Novel Programmable Endonuclease Systems Open
Argonautes are nucleases that can be programmed by short oligonucleotides to cleave complementary sequences. Here, we performed an unbiased bioinformatic search to mine bacterial genomes for prokaryotic Argonautes (pAgos) harboring a PIWI …
View article: Adeno-associated Virus (AAV) Capsid Chimeras with Enhanced Infectivity Reveal a Core Element in the AAV Genome Critical for both Cell Transduction and Capsid Assembly
Adeno-associated Virus (AAV) Capsid Chimeras with Enhanced Infectivity Reveal a Core Element in the AAV Genome Critical for both Cell Transduction and Capsid Assembly Open
A major hurdle to the therapeutic potential of AAV in gene therapy lies in achieving clinically meaningful AAV doses, and secondarily, the ability to manufacture commercially viable titers of AAV to support this. By virtue of neutralizing …
View article: A Novel Cell Therapy for COVID-19 and Potential Future Pandemics: Virus Induced Lymphocytes (VIL)
A Novel Cell Therapy for COVID-19 and Potential Future Pandemics: Virus Induced Lymphocytes (VIL) Open
The a priori T cell repertoire and immune response against SARS-CoV-2 viral antigens may explain the varying clinical course and prognosis of patients having a mild COVID-19 infection as opposed to those developing more fulminant multisyst…
View article: 333 Targeting the apical intracellular checkpoint CISH unleashes T cell neoantigen reactivity and effector program
333 Targeting the apical intracellular checkpoint CISH unleashes T cell neoantigen reactivity and effector program Open
Background Neoantigen-specific T cells isolated from tumors have shown promise clinically but fail to consistently elicit durable tumor regression. Expression of the intracellular checkpoint CISH is elevated in human tumor infiltrating lym…
View article: AAV Capsid Chimeras with Enhanced Infectivity reveal a core element in the AAV Genome critical for both Cell Transduction and Capsid Assembly
AAV Capsid Chimeras with Enhanced Infectivity reveal a core element in the AAV Genome critical for both Cell Transduction and Capsid Assembly Open
Adeno-associated viruses (AAV) have attracted significant attention in the field of gene and cell therapy due to highly effective delivery of therapeutic genes into human cells. The ability to generate recombinant AAV vectors compromised o…
View article: Internal checkpoint regulates T cell neoantigen reactivity and susceptibility to PD1 blockade
Internal checkpoint regulates T cell neoantigen reactivity and susceptibility to PD1 blockade Open
While neoantigen-specific tumor infiltrating lymphocytes (TIL) can be derived from in antigen-expressing tumors, their adoptive transfer fails to consistently elicit durable tumor regression. There has been much focus on the role of activa…
View article: Internal checkpoint regulates T cell neoantigen reactivity and susceptibility to PD1 blockade
Internal checkpoint regulates T cell neoantigen reactivity and susceptibility to PD1 blockade Open
While neoantigen-specific tumor infiltrating lymphocytes (TIL) can be derived from in antigen-expressing tumors, their adoptive transfer fails to consistently elicit durable tumor regression. There has been much focus on the role of activa…
View article: MicroRNA-155 is essential for the optimal proliferation and survival of plasmablast B cells
MicroRNA-155 is essential for the optimal proliferation and survival of plasmablast B cells Open
A fast antibody response can be critical to contain rapidly dividing pathogens. This can be achieved by the expansion of antigen-specific B cells in response to T-cell help followed by differentiation into plasmablasts. MicroRNA-155 (miR-1…