Tomáš Obšil
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View article: Look for the Scaffold: Multifaceted Regulation of Enzyme Activity by 14-3-3 Proteins
Look for the Scaffold: Multifaceted Regulation of Enzyme Activity by 14-3-3 Proteins Open
Enzyme activity is regulated by several mechanisms, including phosphorylation. Phosphorylation is a key signal transduction process in all eukaryotic cells and is thus crucial for virtually all cellular processes. In addition to its direct…
View article: The minimal membrane requirements for BAX-induced pore opening upon exposure to oxidative stress
The minimal membrane requirements for BAX-induced pore opening upon exposure to oxidative stress Open
Perforation of the outer mitochondrial membrane triggered by BAX and facilitated by its main activator cBID is a fundamental process in cell apoptosis. Here, we employ a newly designed correlative approach based on a combination of a fluor…
View article: The cryo-EM structure of ASK1 reveals an asymmetric architecture allosterically modulated by TRX1
The cryo-EM structure of ASK1 reveals an asymmetric architecture allosterically modulated by TRX1 Open
Apoptosis signal-regulating kinase 1 (ASK1) is a crucial stress sensor, directing cells toward apoptosis, differentiation, and senescence via the p38 and JNK signaling pathways. ASK1 dysregulation has been associated with cancer and inflam…
View article: Author Response: The cryo-EM structure of ASK1 reveals an asymmetric architecture allosterically modulated by TRX1
Author Response: The cryo-EM structure of ASK1 reveals an asymmetric architecture allosterically modulated by TRX1 Open
Full text Figures and data Peer review Side by side Abstract eLife assessment Introduction Results Discussion Materials and methods Data availability References Article and author information Metrics Abstract Apoptosis signal-regulating ki…
View article: Author Response: The cryo-EM structure of ASK1 reveals an asymmetric architecture allosterically modulated by TRX1
Author Response: The cryo-EM structure of ASK1 reveals an asymmetric architecture allosterically modulated by TRX1 Open
Apoptosis signal-regulating kinase 1 (ASK1) is a crucial stress sensor, directing cells towards apoptosis, differentiation and senescence via the p38 and JNK signaling pathways. ASK1 dysregulation has been associated with cancer and inflam…
View article: Reviewer #1 (Public Review): The cryo-EM structure of ASK1 reveals an asymmetric architecture allosterically modulated by TRX1
Reviewer #1 (Public Review): The cryo-EM structure of ASK1 reveals an asymmetric architecture allosterically modulated by TRX1 Open
Apoptosis signal-regulating kinase 1 (ASK1) is a crucial stress sensor, directing cells towards apoptosis, differentiation and senescence via the p38 and JNK signaling pathways. ASK1 dysregulation has been associated with cancer and inflam…
View article: The cryo-EM structure of ASK1 reveals an asymmetric architecture allosterically modulated by TRX1
The cryo-EM structure of ASK1 reveals an asymmetric architecture allosterically modulated by TRX1 Open
Apoptosis signal-regulating kinase 1 (ASK1) is a crucial stress sensor, directing cells towards apoptosis, differentiation and senescence via the p38 and JNK signaling pathways. ASK1 dysregulation has been associated with cancer and inflam…
View article: Reviewer #2 (Public Review): The cryo-EM structure of ASK1 reveals an asymmetric architecture allosterically modulated by TRX1
Reviewer #2 (Public Review): The cryo-EM structure of ASK1 reveals an asymmetric architecture allosterically modulated by TRX1 Open
Apoptosis signal-regulating kinase 1 (ASK1) is a crucial stress sensor, directing cells towards apoptosis, differentiation and senescence via the p38 and JNK signaling pathways. ASK1 dysregulation has been associated with cancer and inflam…
View article: The cryo-EM structure of ASK1 reveals an asymmetric architecture allosterically modulated by TRX1
The cryo-EM structure of ASK1 reveals an asymmetric architecture allosterically modulated by TRX1 Open
Apoptosis signal-regulating kinase 1 (ASK1) is a crucial stress sensor, directing cells toward apoptosis, differentiation, and senescence via the p38 and JNK signaling pathways. ASK1 dysregulation has been associated with cancer and inflam…
View article: The yeast 14-3-3 proteins Bmh1 and Bmh2 regulate key signaling pathways
The yeast 14-3-3 proteins Bmh1 and Bmh2 regulate key signaling pathways Open
Cell signaling regulates several physiological processes by receiving, processing, and transmitting signals between the extracellular and intracellular environments. In signal transduction, phosphorylation is a crucial effector as the most…
View article: The cryo-EM structure of ASK1 reveals an asymmetric architecture allosterically modulated by TRX1
The cryo-EM structure of ASK1 reveals an asymmetric architecture allosterically modulated by TRX1 Open
Apoptosis signal-regulating kinase 1 (ASK1) is a crucial stress sensor, directing cells towards apoptosis, differentiation and senescence via the p38 and JNK signaling pathways. ASK1 dysregulation has been associated with cancer and inflam…
View article: 14‐3‐3 protein inhibits <scp>CaMKK1</scp> by blocking the kinase active site with its last two C‐terminal helices
14‐3‐3 protein inhibits <span>CaMKK1</span> by blocking the kinase active site with its last two C‐terminal helices Open
Ca 2+ /CaM‐dependent protein kinase kinases 1 and 2 (CaMKK1 and CaMKK2) phosphorylate and enhance the catalytic activity of downstream kinases CaMKI, CaMKIV, and protein kinase B. Accordingly, CaMKK1 and CaMKK2 regulate key physiological a…
View article: Data from Hippo/Mst1 Stimulates Transcription of the Proapoptotic Mediator <i>NOXA</i> in a FoxO1-Dependent Manner
Data from Hippo/Mst1 Stimulates Transcription of the Proapoptotic Mediator <i>NOXA</i> in a FoxO1-Dependent Manner Open
The proapoptotic protein Noxa, a member of the BH3-only Bcl-2 protein family, can effectively induce apoptosis in cancer cells, although the relevant regulatory pathways have been obscure. Previous studies of the cytotoxic effects of α-toc…
View article: Supplementary Figure Legends 1-2 from Hippo/Mst1 Stimulates Transcription of the Proapoptotic Mediator <i>NOXA</i> in a FoxO1-Dependent Manner
Supplementary Figure Legends 1-2 from Hippo/Mst1 Stimulates Transcription of the Proapoptotic Mediator <i>NOXA</i> in a FoxO1-Dependent Manner Open
Supplementary Figure Legends 1-2 from Hippo/Mst1 Stimulates Transcription of the Proapoptotic Mediator NOXA in a FoxO1-Dependent Manner
View article: Data from Hippo/Mst1 Stimulates Transcription of the Proapoptotic Mediator <i>NOXA</i> in a FoxO1-Dependent Manner
Data from Hippo/Mst1 Stimulates Transcription of the Proapoptotic Mediator <i>NOXA</i> in a FoxO1-Dependent Manner Open
The proapoptotic protein Noxa, a member of the BH3-only Bcl-2 protein family, can effectively induce apoptosis in cancer cells, although the relevant regulatory pathways have been obscure. Previous studies of the cytotoxic effects of α-toc…
View article: Supplementary Figures 1-2 from Hippo/Mst1 Stimulates Transcription of the Proapoptotic Mediator <i>NOXA</i> in a FoxO1-Dependent Manner
Supplementary Figures 1-2 from Hippo/Mst1 Stimulates Transcription of the Proapoptotic Mediator <i>NOXA</i> in a FoxO1-Dependent Manner Open
Supplementary Figures 1-2 from Hippo/Mst1 Stimulates Transcription of the Proapoptotic Mediator NOXA in a FoxO1-Dependent Manner
View article: Supplementary Figure Legends 1-2 from Hippo/Mst1 Stimulates Transcription of the Proapoptotic Mediator <i>NOXA</i> in a FoxO1-Dependent Manner
Supplementary Figure Legends 1-2 from Hippo/Mst1 Stimulates Transcription of the Proapoptotic Mediator <i>NOXA</i> in a FoxO1-Dependent Manner Open
Supplementary Figure Legends 1-2 from Hippo/Mst1 Stimulates Transcription of the Proapoptotic Mediator NOXA in a FoxO1-Dependent Manner
View article: Supplementary Figures 1-2 from Hippo/Mst1 Stimulates Transcription of the Proapoptotic Mediator <i>NOXA</i> in a FoxO1-Dependent Manner
Supplementary Figures 1-2 from Hippo/Mst1 Stimulates Transcription of the Proapoptotic Mediator <i>NOXA</i> in a FoxO1-Dependent Manner Open
Supplementary Figures 1-2 from Hippo/Mst1 Stimulates Transcription of the Proapoptotic Mediator NOXA in a FoxO1-Dependent Manner
View article: Structural insights into the functional roles of 14-3-3 proteins
Structural insights into the functional roles of 14-3-3 proteins Open
Signal transduction cascades efficiently transmit chemical and/or physical signals from the extracellular environment to intracellular compartments, thereby eliciting an appropriate cellular response. Most often, these signaling processes …
View article: Lengthening the Guanidine–Aryl Linker of Phenylpyrimidinylguanidines Increases Their Potency as Inhibitors of FOXO3-Induced Gene Transcription
Lengthening the Guanidine–Aryl Linker of Phenylpyrimidinylguanidines Increases Their Potency as Inhibitors of FOXO3-Induced Gene Transcription Open
Increased FOXO3 nuclear localization is involved in neuroblastoma chemoresistance and tumor angiogenesis. Accordingly, FOXO3 inhibition is a promising strategy for boosting antitumor immune responses and suppressing FOXO3-mediated therapy …
View article: <scp>FOXO4</scp> interacts with p53 <scp>TAD</scp> and <scp>CRD</scp> and inhibits its binding to <scp>DNA</scp>
<span>FOXO4</span> interacts with p53 <span>TAD</span> and <span>CRD</span> and inhibits its binding to <span>DNA</span> Open
Transcription factor p53 protects cells against tumorigenesis when subjected to various cellular stresses. Under these conditions, p53 interacts with transcription factor Forkhead box O (FOXO) 4, thereby inducing cellular senescence by upr…
View article: Structural Insights Support Targeting ASK1 Kinase for Therapeutic Interventions
Structural Insights Support Targeting ASK1 Kinase for Therapeutic Interventions Open
Apoptosis signal-regulating kinase (ASK) 1, a member of the mitogen-activated protein kinase kinase kinase (MAP3K) family, modulates diverse responses to oxidative and endoplasmic reticulum (ER) stress and calcium influx. As a crucial cell…
View article: 14-3-3 proteins inactivate DAPK2 by promoting its dimerization and protecting key regulatory phosphosites
14-3-3 proteins inactivate DAPK2 by promoting its dimerization and protecting key regulatory phosphosites Open
Death-associated protein kinase 2 (DAPK2) is a CaM-regulated Ser/Thr protein kinase, involved in apoptosis, autophagy, granulocyte differentiation and motility regulation, whose activity is controlled by autoinhibition, autophosphorylation…
View article: Study of protein–protein interactions of human E3 ubiquitin ligase NEDD4-2 using fluorescence spectroscopy
Study of protein–protein interactions of human E3 ubiquitin ligase NEDD4-2 using fluorescence spectroscopy Open
Neural precursor cells expressed developmentally downregulated protein 4-2 (Nedd4-2) plays a key role in the ubiquitination process, which leads to the endocytosis and degradation of its downstream target molecules such as membrane protein…
View article: 14-3-3 protein dependent modulation of ubiquitin ligase Nedd4-2
14-3-3 protein dependent modulation of ubiquitin ligase Nedd4-2 Open
Neural precursor cell expressed developmentally down-regulated 4 ligase (Nedd4-2) is an E3 ubiquitin ligase that targets proteins for ubiquitination and endocytosis, thereby regulating numerous ion channels, membrane receptors and tumor su…
View article: Biophysical characterization of the interaction between Forkhead box O4 (FOXO4) and p53 transcription factors
Biophysical characterization of the interaction between Forkhead box O4 (FOXO4) and p53 transcription factors Open
The transcription factor p53 controls numerous cellular processes including apoptosis, senescence, DNA repair, and tumor suppression [1].The function of p53 is closely intertwined with Forkhead box O (FOXO) transcription factors and FOXO p…