Explanipedia

Rapid apical actin filament turnover is required for non-centrosomal microtubule organization in epithelium Open

A. Babu, Sachin Muralidharan, Konstantin Kogan, Tommi Kotila, Jaakko Mattila , et al. · 2025

Epithelial cells rely on the precise coordination of actin and microtubule cytoskeletons to maintain their polarized structure and function. Microtubules form longitudinal non-centrosomal arrays that are essential for organelle positioning…

Leishmania profilin interacts with actin through an unusual structural mechanism to control cytoskeletal dynamics in parasites Open

Andrea Vizcaíno-Castillo, Tommi Kotila, Konstantin Kogan, Ryuji Yanase, Juna Como , et al. · 2024

Recycling of the actin monomer pool limits the lifetime of network turnover Open

Alexandra Colin, Tommi Kotila, Christophe Guérin, Magali Orhant‐Prioux, Benoît Vianay , et al. · 2023

Intracellular organization is largely mediated by actin turnover. Cellular actin networks continuously assemble and disassemble, while maintaining their overall appearance. This behavior, called “dynamic steady state,” allows cells to sens…

Unusual actin-binding mechanism and the role of profilin in actin dynamics of trypanosomatid parasites Open

Andrea Vizcaíno-Castillo, Tommi Kotila, Konstantin Kogan, Ryuji Yanase, Juna Como , et al. · 2023

Diseases caused by Leishmania , and Trypanosoma parasites, such as leishmaniasis and African sleeping sickness, are a major health problem in tropical countries. Due to their complex life cycle involving both vertebrate and insect hosts, a…

Structural basis underlying specific biochemical activities of non-muscle tropomyosin isoforms Open

Muniyandi Selvaraj, Shrikant Babanrao Kokate, Gabriella Reggiano, Konstantin Kogan, Tommi Kotila , et al. · 2022

The actin cytoskeleton is critical for cell migration, morphogenesis, endocytosis, organelle dynamics, and cytokinesis. To support diverse cellular processes, actin filaments form a variety of structures with specific architectures and dyn…

Recycling limits the lifetime of actin turnover Open

Alexandra Colin, Tommi Kotila, Christophe Guérin, Magali Orhant‐Prioux, Benoît Vianay , et al. · 2022

Intracellular organization is largely mediated by the actin turnover. Cellular actin networks consume matter and energy to sustain their dynamics, while maintaining their appearance. This behavior, called ‘dynamic steady state’, enables ce…

Structural basis of rapid actin dynamics in the evolutionarily divergent Leishmania parasite Open

Tommi Kotila, Hugo Wioland, Muniyandi Selvaraj, Konstantin Kogan, Lina Antenucci , et al. · 2022

Structural basis underlying specific biochemical activities of non-muscle tropomyosin isoforms Open

Muniyandi Selvaraj, Shrikant Babanrao Kokate, Gabriella Reggiano, Konstantin Kogan, Tommi Kotila , et al. · 2022

SUMMARY The actin cytoskeleton is critical for cell migration, morphogenesis, endocytosis, organelle dynamics, and cytokinesis. To support diverse cellular processes, actin filaments form a variety of structures with specific architectures…

Mechanism of Borrelia immune evasion by FhbA-related proteins Open

Konstantin Kogan, Karita Haapasalo, Tommi Kotila, Robin Moore, Pekka Lappalainen , et al. · 2022

Immune evasion facilitates survival of Borrelia , leading to infections like relapsing fever and Lyme disease. Important mechanism for complement evasion is acquisition of the main host complement inhibitor, factor H (FH). By determining t…

Publisher Correction: Twinfilin uncaps filament barbed ends to promote turnover of lamellipodial actin networks Open

Markku Hakala, Hugo Wioland, Mari Tolonen, Tommi Kotila, Antoine Jégou , et al. · 2021

A functional family of fluorescent nucleotide analogues to investigate actin dynamics and energetics Open

Jessica Colombo, Adrien Antkowiak, Konstantin Kogan, Tommi Kotila, Jenna Elliott , et al. · 2021

Regulation of INF2-mediated actin polymerization through site-specific lysine acetylation of actin itself Open

Mu A, Tak Shun Fung, Lisa M. Francomacaro, Thao N. Huynh, Tommi Kotila , et al. · 2019

Significance Tight regulation is required to control biochemical reactions in cells so that these reactions can be activated precisely when and where needed. Our work focuses on actin, a protein that polymerizes into filaments in at least …

Mechanism of synergistic actin filament pointed end depolymerization by cyclase-associated protein and cofilin Open

Tommi Kotila, Hugo Wioland, Giray Enkavi, Konstantin Kogan, Ilpo Vattulainen , et al. · 2019

HFD-bound and HFD-free simulation systems presented in the paper; see table_of_simulations.pdf Contents of the simulation_archive: _FORCEFIELD: Force field parameters and topologies in GROMACS format amber-ff14SB.ff: The r…

Mechanism of synergistic actin filament pointed end depolymerization by cyclase-associated protein and cofilin Open

Tommi Kotila, Hugo Wioland, Giray Enkavi, Konstantin Kogan, Ilpo Vattulainen , et al. · 2019

The ability of cells to generate forces through actin filament turnover was an early adaptation in evolution. While much is known about how actin filaments grow, mechanisms of their disassembly are incompletely understood. The best-charact…

Structural basis of actin monomer re-charging by cyclase-associated protein Open

Tommi Kotila, Konstantin Kogan, Giray Enkavi, Siyang Guo, Ilpo Vattulainen , et al. · 2018

Actin polymerization powers key cellular processes, including motility, morphogenesis, and endocytosis. The actin turnover cycle depends critically on “re-charging” of ADP-actin monomers with ATP, but whether this reaction requires dedicat…

Revolutionising the design and analysis of protein engineering experiments using fractional factorial design Open

Steven P. D. Harborne, Duncan Wotherspoon, Jessica Michie, Alasdair McComb, Tommi Kotila , et al. · 2018

Protein engineering is one of the foundations of biotechnology, used to increase protein stability, re-assign the catalytic properties of enzymes or increase the interaction affinity between antibody and target. To date, strategies for pro…

Structural Basis Of Actin Monomer Re-Charging By Cyclase-Associated Protein Open

Tommi Kotila, Konstantin Kogan, Giray Enkavi, Siyang Guo, Ilpo Vattulainen , et al. · 2018

1) table_of_simulations.pdf: table of simulations 2) toppar_HIC.str: methylhistidine (HIC) topologies and parameters -prepared based on analogy -to be used with top_all36_prot.rtf and par_all36_prot.prm 3) simulation_archive.tar.gz The Con…

Structural Basis Of Actin Monomer Re-Charging By Cyclase-Associated Protein Open

Tommi Kotila, Konstantin Kogan, Giray Enkavi, Siyang Guo, Ilpo Vattulainen , et al. · 2018

1) table_of_simulations.pdf: table of simulations 2) toppar_HIC.str: methylhistidine (HIC) topologies and parameters -prepared based on analogy -to be used with top_all36_prot.rtf and par_all36_prot.prm 3) simulation_archive.tar.gz The Con…

Crystal structure of a tripartite complex between C3dg, C-terminal domains of factor H and OspE of Borrelia burgdorferi Open

R. Kolodziejczyk, Kornelia M. Mikula, Tommi Kotila, Vincent L. G. Postis, T. Sakari Jokiranta , et al. · 2017

Complement is an important part of innate immunity. The alternative pathway of complement is activated when the main opsonin, C3b coats non-protected surfaces leading to opsonisation, phagocytosis and cell lysis. The alternative pathway is…

UNC-45a promotes myosin folding and stress fiber assembly Open

Jaakko Lehtimäki, Aidan M. Fenix, Tommi Kotila, Giuseppe Balistreri, Lassi Paavolainen , et al. · 2017

Contractile actomyosin bundles, stress fibers, are crucial for adhesion, morphogenesis, and mechanosensing in nonmuscle cells. However, the mechanisms by which nonmuscle myosin II (NM-II) is recruited to those structures and assembled into…

Tropomyosin Isoforms Specify Functionally Distinct Actin Filament Populations In Vitro Open

Gergana Gateva, Elena Kremneva, Theresia Reindl, Tommi Kotila, Konstantin Kogan , et al. · 2017

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