Tomoki Oi
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View article: Plasmid-free CRISPR/Cas9 genome editing in<i>Saccharomyces cerevisiae</i>
Plasmid-free CRISPR/Cas9 genome editing in<i>Saccharomyces cerevisiae</i> Open
The current CRISPR/Cas9 systems in the yeast Saccharomyces cerevisiae cannot be considered a non-genetic modification technology because it requires the introduction of Cas9 and sgRNA into yeast cells using plasmid expression systems. Our …